Screening and identification of cucumber germplasm and rootstock resistance against the root-knot nematode (Meloidogyne incognita)

Li, X Z; Chen, S X

doi:10.4238/gmr16029383

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…However, cucumber is high sensitive to low/high temperature, drought/waterlogging and salt stresses that cause significant reductions in yields (Liu et al 2013Ma et al 2018;Xu et al 2017Xu et al , 2018. In addition, a wide range of soilborne diseases (pathogenes), viruses and nematodes are one of the main constraints on cucumber production (Li and Chen 2017;Liang et al 2019;Liu et al 2017). Although grafting of cucumber plants onto cucurbitaceous rootstocks is an efficiency way to improve abiotic/biotic stress, this technique is labor-intensive (Xu et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

Fan

Zhou

et al. 2020

Plant Cell Tiss Organ Cult

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Agrobacterium rhizogenes-mediated transformation is widely used in different species with various purposes. The development of composite plants (wild-type shoot with transgenic roots) has been a milestone for functional characterization of genes. Previously, composite plants were generated by two steps from inducing of hairy roots to growing in the growth medium. Hairy roots were induced in an induction medium and the growth of composite plants generated were in another different growth medium. The composite plants produced was subject to transplanting. Here, we describe an improved and optimized protocol for generation of composite plant achieved by one-step in cucumber, which has not been reported previously in living plants. Incubation of explants post inoculation to induce transgenic roots and the growth of rooted explants were in the same medium. The primary root of 5-day-old seedling was excised and the slant cut of residual hypocotyl with 1 cm length was inoculated with A. rhizogenes harboring the desired gene construct followed by directly planted into a pot with wet sterile vermiculite. More than 90% of the infected seedlings can produce positive transgenic root. In addition, we further used the one-step transformation protocol to analyze the function of Arabidopsis YAO promoter. The result indicated that pYAO::GUS was highly conserved expression in whole root and high activity in the root tips. Therefore, a fast, expedient, high efficient, and one-step transformation method of composite cucumber produced is established, which is suitable for promoter functional analysis and other root-related events. Key messageA highly reliable, facile and one-step generation of composite cucumber plants (with transgenic roots and wild-type shoot) protocol performed effectively in different genotypes cucumber using Agrobacterium rhizogenes-mediated transformation.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Introductionmentioning

confidence: 99%

A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

Fan

Zhou

et al. 2020

Plant Cell Tiss Organ Cult

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“…Grafting is a traditional technique and widely implemented in modern agriculture to control soil-borne diseases caused by bacteria, fungi, oomycetes, viruses or root-knot nematodes of different crops in a sustainable and environmentally friendly approach (Nawaz et al, 2016;Li and Chen, 2017;Li and Zhao, 2021;Thies, 2021). Briefly, this technology is commonly accomplished by connecting two plant segments, the upper one named the scion and the lower part known as the rootstock.…”

Section: Grafting Technologymentioning

confidence: 99%

Exploring new strategies in diseases resistance of horticultural crops

Zhang

Wang

et al. 2022

Front. Sustain. Food Syst.

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Horticultural crops are susceptible to various biotic stressors including fungi, oomycetes, bacteria, viruses, and root-knot nematodes. These pathogens limit the growth, development, yield, and quality of horticultural crops, and also limit their adaptability and geographic distribution. The continuous cropping model in horticultural facilities exacerbates soil-borne diseases, and severely restricts yield, quality, and productivity. Recent progress in the understanding of mechanisms that confer tolerance to different diseases through innovative strategies including host-induced gene silencing (HIGS), targeting susceptibility genes, and rootstocks grafting applications are reviewed to systematically explore the resistance mechanisms against horticultural plant diseases. Future work should successfully breed resistant varieties using these strategies combined with molecular biologic methods.

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“…Hem dünyada hem de ülkemizde örtüaltı üretim alanlarında domatesten sonra üretimi en fazla yapılan sebze türüdür (Kurtar ve ark. 2017, Li andChen 2017). Ülkemiz yaklaşık 1.78 milyon ton hıyar üretim miktarı ile dünyada ikinci sırada yer almaktadır (Anonymous 2017).…”

Section: öZunclassified

Bazı hıyar çeşitlerinin kök-ur nematodları Meloidogyne arenaria ve M. incognita’ya konukçu reaksiyonu

Aydınlı

İnce²,

Mennan

2017

Bitki Koruma Bülteni

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Host reaction of some cucumber cultivars to Meloidogyne arenaria and M. incognita Cultivation of resistant or poor hosts to root-knot nematodes provides significant advantages in nematode-infested areas. In this respect, it is important to know the reactions of the different varieties to the root nematodes. In this study, the host status of 15 cucumber cultivars (Cucumis sativus), which are widely used commercially, to M. arenaria and M. incognita was evaluated by a pot experiment in a greenhouse. Although nematode infection increased root weights of all cultivars compared to the controls, these increases were significant (P≤0.05) only in 1 cultivar (Toskono) and other 2 cultivars (Everest and Seyhan) for M. arenaria and M. incognita, respectively. These cultivars also had the highest formation of galls for related nematode species among all tested. All of the cucumber cultivars were susceptible to different levels of both nematode species, and the lowest gall index according to the 0-10 gall index were detected in Almino with 2.8 for M. arenaria and Venüs with 3.8 for M. incognita. The highest and the lowest reproduction factors on cultivars were 5.84 and 17.16 for M. arenaria and 8.28 and 17.84 for M. incognita, respectively. Nine cultivars showed different reaction at the significant level in terms of reproductive factor compared to the nematode species and 8 of them also differed based on the galling index.

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Screening and identification of cucumber germplasm and rootstock resistance against the root-knot nematode (Meloidogyne incognita)

Cited by 10 publications

References 18 publications

A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

A fast, simple, high efficient and one-step generation of composite cucumber plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

Exploring new strategies in diseases resistance of horticultural crops

Bazı hıyar çeşitlerinin kök-ur nematodları Meloidogyne arenaria ve M. incognita’ya konukçu reaksiyonu

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