Screening and Identification of Brown Planthopper Resistance Genes OsCM9 in Rice

Abstract

The brown planthopper (BPH) is a major pest that causes serious damage to rice in countries with mild climates, including Korea, Japan, China, and Vietnam. Although a large number of BPH-resistant genes have been reported, BPH has recently evolved rapidly and in a variable manner due to rapid climate change and an unpredictable environment. The synthesis of secondary metabolites in plants is essential, as it provides resistance to various stressors, including pests such as BPH. For QTL mapping, a Samgang/Nagdong double haploid (SNDH) 113 population was used. The BPH resistance gene was screening by measure the resistance score using the phenotype that appeared after BPH inoculation in the 113 SNDH population. QTL mapping was used, and SSR marker RM584-RM225 of chromosome 6 and SSR marker RM331-S8024 of chromosome 8 were commonly identified. These locations contained transcription factors, phytohormones, signaling molecules, kinases, and secondary metabolites that defend plants from stressors in the environment. This region of chromosome 8 also contained a sequence similar to that of rice Choristmate mutase (OsCM9). CM is a gene that is not only found in rice, but also in thale cress (Arabidopsis thaliana (L.) Heynh.), maize (Zea mays L.), and soybean (Glycine max (L.) Merr.). After inoculation with BPH, the relative expression levels of OsCM9 were greater in the line resistant to BPH than in the line susceptible to BPH. The newly identified BPH-resistant gene OsCM9 can be used for the development of rice varieties that are capable of resisting sudden damage due to BPH, as the evolution of BPH due to climate change has had negative impacts on rice crops.

Genotype and Phenotype Interaction between OsWKRYq6 and BLB after Xanthomonas oryzae pv. Oryzae Inoculation in the Field

“…In addition, it was said that both the RIL group and the DH group were effective in performing QTL mapping. Some genes already discovered through QTL mapping were analyzed for expression through the CNDH line, and the functions of the genes were identified [ 26 , 27 ].…”

Over-Expression of Chorismate Mutase Enhances the Accumulation of Salicylic Acid, Lignin, and Antioxidants in Response to the White-Backed Planthopper in Rice Plants

“…Currently, rice planthoppers, primarily the WBPH, are the most devastating insect pests, and they cause severe damage to plants by direct feeding or indirect disease transmission, which leads to severe yield losses [ 36 , 37 ]. Previously, we identified and mapped the CM gene, which is resistant to brown planthopper (BPH), using QTL analysis and developed a transgenic rice line [ 38 ]. In the current study, we characterized the CM gene to establish prolonged resistance of the rice cultivar against WBPH and to contribute to sustainable rice production.…”

Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field

“…Generation has progressed in the field of Kyungpook National University since 2010, and it has a very diverse phenotype and genotype. The 120 CNDH population is currently used as an intermediate model and is also used to analyze the functions of newly discovered genes [33,34]. After inoculating the 120 CNDH population with Xoo, which induced BLB, leaf analysis was performed using OCT.…”