Screening and Identification of Anti-Inflammatory Compounds from Erdong Gao via Multiple-Target-Cell Extraction Coupled with HPLC-Q-TOF-MS/MS and Their Structure–Activity Relationship

Abstract

Erdong Gao (EDG), consisting equally of roots of Asparagi Radix and Ophiopogonis Radix, is a well-known traditional Chinese formulation that has been used to treat cough and throat pain for centuries. However, the bioactive components in EDG remain to be elucidated. In this study, a rapid and effective method involving live cell bio-specific extraction and HPLC-Q-TOF-MS/MS was established to rapidly screen and identify the anti-inflammatory compounds of an EDG extract. One hundred and twenty-four components were identified in EDG extract using HPLC-Q-TOF-MS/MS analysis. After co-incubation with 16HBE, HPAEpiCs and HUVECs, which have been validated as the key target cells for pulmonary diseases, sixteen components were demonstrated to exhibit an affinity for binding to them. Furthermore, fifteen components were subsequently verified to exert anti-inflammatory effects on lipopolysaccharide (LPS)-induced 16HBE, HPAEpiCs and HUVECs via inhibiting the release of TNF-α and IL-6, indicating that nine steroidal saponins may possess potential for the treatment of lung-related diseases. Taken together, our study provides evidence that live cell biospecific extraction combined with the HPLC-Q-TOF-MS/MS technique was an efficient method for rapid screening potential bioactive components in traditional Chinese medicines and the structure activity relationship of steroidal saponins in EDG was summarized for the first time.

UPLC-Q-TOF-MSE Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum

“…Additionally, research has demonstrated that the chemical constituents of phenolic acids possess strong antioxidant capacity. 24,25 It exhibits antioxidant, antiinflammatory, antitumor, and other pharmacological effects, which may be linked to the content and type of phenolic acids present in T. hemsleyanum . Polysaccharide is also a crucial active compound in T. hemsleyanum and is one of its saccharides.…”

“…Ultra-performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS E ) is a useful method for this purpose due to its high sensitivity, selectivity, and ability to provide complete and high-quality mass spectrometry data. 4,5 This method is simple and efficient 6,7 and has been successfully applied in many fields, 8 including Chinese herbal medicine, 9–12 natural product research and development, 13,14 microorganisms, 15 and the food industry. It serves as a highly effective tool for elucidating the chemical composition profile of substances.…”

Characterization of Seven New Steroidal Saponins from Korean Oat Cultivars by UPLC-QTOF-MS and UPLC-MS/MS

“…In addition, structures containing two or more sugar chains at the C-3 position (especially the additional rhamnose units), ,, and the 25­( R / S )-configuration (25 R > 25 S : A549, Caski, HepG2, and MCF-7; , 25 S > 25 R : BEL-7402, HT-29, Hela, MDA-MB-468, BT549, and SW620 cell lines , ) were considered important for cytotoxicity. Moreover, the aforementioned factors also influenced anti-inflammatory, − antiatherosclerotic, antidiabetic, and antiosteosarcoma activities. However, studies of oat saponins, including the new saponins identified in this study, are still lacking.…”