Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum

Abstract

In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P <0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia.

Allelic segregation of sex‐linked microsatellite markers in Nile tilapia (Oreochromis niloticus) and validation of inheritance in YY population

“…(Cnaani et al, 2008;Lee et al, 2005). Very recent studies by Zhu et al (2016) One of the paternal alleles (236 bp long) of LG1 UNH995 was always associated with male progeny, while the other was associated with both male and female progeny (Khan, 2011;Palaiokostas et al, 2015). In the present study, although UNH995 was found to be polymorphic, it could not distinguish between females (XX) and males genotypes (both XY and YY).…”

“…Allelic inheritance studies have found association of a number of DNA markers with sex in some tilapia species and their hybrids, for example, UNH995 on LG1 in O. niloticus and O. aureus ; GM201 on LG1 in O. niloticus, O. aureus and O. mossambicus ; UNH148 on LG1 in O. niloticus and O. mossambicus ; and GM345 on LG3 in O. aureus and O. karongae (Cnaani et al, ; Lee et al, ). Very recent studies by Zhu et al () revealed that one of the LG3 markers, UNH168, had been associated ( p < .001) with the phenotypic sex in the experimental population of Wami tilapia, O. u. hornorum . Only a 99‐bp allele was detected in the female samples, while three alleles 141, 149 and 157‐bp were present in both male and female samples.…”

“…Ocalewicz, Mota‐Velasco, Campos‐Ramos, & Penman, ) where the largest chromosomes, equivalent to LG3, had been considered as sex chromosomes due to a region of incomplete or delayed chromosome pairing observed in the synaptonemal complex (SC) in meiotic chromosomes of Nile tilapia. Hybridization signals of bacterial artificial chromosome (BAC) probe have been detected recently on the long arm of the largest chromosome pair (chromosome 1, LG3) of another tilapia species, O. u. hornorum without any visible difference of the hybridized signals between the male and female tilapia suggesting the identities of the microsatellite sequences of both sexes (Zhu et al, ).…”

“…Hybridization signals of bacterial artificial chromosome (BAC) probe have been detected recently on the long arm of the largest chromosome pair (chromosome 1, LG3) of another tilapia species,O. u. hornorum without any visible difference of the hybridized signals between the male and female tilapia suggesting the identities of the microsatellite sequences of both sexes(Zhu et al, 2016).…”

Diferenciación genética de tilapia roja y gris (Oreochromis niloticus) mediante microsatélites y marcadores SCAR como indicadores del sexo genético

“…En O.hornorum, el microsatélite UNH168 fue consistente en la amplificación y asociación al sexo fenotípico femenino, mientras que otros microsatélites estaban presentes en muestras de ambos sexos (Zhu et al 2016). Los marcadores microsatélites UNH995 y UNH104 relacionados con la diferenciación del sexo en O. niloticus fueron determinados por Lee et al (2003) y el microsatélite UNH898 fue propuesto como el mejor marcador asociado a los genes determinantes del sexo, por diferenciar machos de hembras con una sensibilidad de 96.71% (Naranjo et al 2015).…”