Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene

Saruta, Masayasu; Takada, Yoshitake; Kikuchi, Akio; Yamada, Tetsusya; Komatsu, Keiichi; Sayama, Takashi; Ishimoto, Masao; Okabe, Akinori

doi:10.1270/jsbbs.61.625

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…Peanut stunt virus (PSV) is a pathogen of plants, especially legumes, which belongs to genus Cucumovirus [ 1 , 2 , 3 , 4 , 5 ]. The PSV genome consists of three (+)single-stranded RNAs and five open reading frames (ORFs) encoding the 1a and 2a components of the viral replicase, the movement protein, the coat protein (CP), and the 2b protein known as a viral silencing suppressor [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

Obrępalska‐Stęplowska

Żmieńko

Wrzesińska

et al. 2018

Viruses

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Peanut stunt virus (PSV) is a widespread pathogen infecting legumes. The PSV strains are classified into four subgroups and some are defined by the association of satellite RNAs (satRNAs). In the case of PSV, the presence of satRNAs alters the symptoms of disease in infected plants. In this study, we elucidated the plant response to PSV-G strain, which occurs in natural conditions without satRNA. However, it was found that it might easily acquire satRNA, which exacerbated pathogenesis in Nicotiana benthamiana. To explain the mechanisms underlying PSV infection and symptoms exacerbation caused by satRNA, we carried out transcriptome profiling of N. benthamiana challenged by PSV-G and satRNA using species-specific microarrays. Co-infection of plants with PSV-G + satRNA increased the number of identified differentially expressed genes (DEGs) compared with the number identified in PSV-G-infected plants. In both treatments, the majority of up-regulated DEGs were engaged in translation, ribosome biogenesis, RNA metabolism, and response to stimuli, while the down-regulated DEGs were required for photosynthesis. The presence of satRNA in PSV-G-infected plants caused different trends in expression of DEGs associated with phosphorylation, ATP binding, and plasma membrane.

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Section: Introductionmentioning

confidence: 99%

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

Obrępalska‐Stęplowska

Żmieńko

Wrzesińska

et al. 2018

Viruses

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“…SMV inoculation was carried out according to Saruta et al (2012) . SMV-infected soybean leaf tissue frozen at –80°C was homogenized in 0.1 M sodium phosphate buffer (pH 7.0) using a ratio of approximately 1 g of infected tissue per 10 mL of buffer.…”

Section: Methodsmentioning

confidence: 99%

A major gene for tolerance to cold-induced seed coat discoloration relieves viral seed mottling in soybean

et al. 2020

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In yellow soybeans, inhibition of seed coat pigmentation by RNA silencing of CHS genes is suppressed by low temperature and a viral suppressor, resulting in ‘cold-induced seed coat discoloration’ and ‘seed mottling’, respectively. Differences exist in the degree of cold-induced seed coat discoloration among Japanese yellow soybean cultivars; for example, Toyomusume is sensitive, Toyohomare has some tolerance, and Toyoharuka is highly tolerant. In this study, we compared the degree of seed mottling severity due to soybean mosaic virus (SMV) among these three soybean cultivars. Obvious differences were found, with the order of severity as follows: Toyohomare > Toyomusume > Toyoharuka. RNA gel blot analysis indicated that CHS transcript abundance in the seed coat, which was increased by SMV infection, was responsible for the severity of seed mottling. Quantitative reverse transcription PCR analysis revealed why mottling was most severe in SMV-infected Toyohomare: the SMV titer in its seed coat was higher than in the other two infected cultivars. We further suggest that a major gene ( Ic ) for tolerance to cold-induced seed coat discoloration can relieve the severity of seed mottling in SMV-infected Toyoharuka.

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“…Sugimoto et al (2008) mapped Rps1d to chromosome 3 using progenies from a cross between 'Tanbakuro' and 'PI 103091' to determine selectable SSR markers (Sugimoto et al 2008). PSV resistance is traced to a single dominant gene, Rpsv1, near Satt435 on chromosome 7 by using RILs with PSV resistance from a cross between 'Hyuga' and 'Enrei' (Saruta et al 2011). Two QTLs for antibiosis resistance to CCW, CCW -1, and CCW -2, both on chromosome 7, have been identified using progenies from a cross between 'Fukuyutaka' and 'Himeshirazu' (Komatsu et al 2005).…”

Section: Progress On Soybean Molecular Breeding In Japanmentioning

confidence: 99%

Impacts of genomic research on soybean improvement in East Asia

Wang

Jiang

et al. 2019

Theor Appl Genet

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It has been commonly accepted that soybean domestication originated in East Asia. Although East Asia has the historical merit in soybean production, the USA has become the top soybean producer in the world since 1950s. Following that, Brazil and Argentina have been the major soybean producers since 1970s and 1990s, respectively. China has once been the exporter of soybean to Japan before 1990s, yet she became a net soybean importer as Japan and the Republic of Korea do. Furthermore, the soybean yield per unit area in East Asia has stagnated during the past decade. To improve soybean production and enhance food security in these East Asian countries, much investment has been made, especially in the breeding of better performing soybean germplasms. As a result, China, Japan, and the Republic of Korea have become three important centers for soybean genomic research. With new technologies, the rate and precision of the identification of important genomic loci associated with desired traits from germplasm collections or mutants have increased significantly. Genome editing on soybean is also becoming more established. The year 2019 marked a new era for crop genome editing in the commercialization of the first genome-edited plant product, which is a high-oleic-acid soybean oil. In this review, we have summarized the latest developments in soybean breeding technologies and the remarkable progress in soybean breeding-related research in China, Japan, and the Republic of Korea. Communicated by Rajeev K. Varshney.

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Screening and genetic analysis of resistance to peanut stunt virus in soybean: identification of the putative Rpsv1 resistance gene

Cited by 7 publications

References 19 publications

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

A major gene for tolerance to cold-induced seed coat discoloration relieves viral seed mottling in soybean

Impacts of genomic research on soybean improvement in East Asia

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