Screening and Evaluation of Human Intestinal Lactobacilli for the Development of Novel Gastrointestinal Probiotics

Kõll, Piret; Mändar, Reet; Smidt, Imbi; Hütt, Pirje; Truusalu, Kai; Mikelsaar, Raik-Hiio; Shchepetova, Jelena; Krogh-Andersen, Kasper; Marcotte, Harold; Hammarström, Lennart; Mikelsaar, Marika

doi:10.1007/s00284-010-9653-y

Cited by 43 publications

(27 citation statements)

References 30 publications

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“…There have been a number of reports for isolating the probiotic lactobacilli from human gastrointestinal tract (Dunne et al, 2001;Koll et al, 2010;Morelli, 2000); none is from healthy babies in Pakistan. With different environments, social set up and immunity in Pakistani babies, we aimed to isolate, identify and characterize probiotic lactobacilli.…”

Section: Introductionmentioning

confidence: 99%

Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan

Nawaz¹,

Wang²,

Zhou³

et al. 2011

Afr. J. Microbiol. Res.

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Probiotic bacteria are used for the protection and improvement of human intestinal microflora with added health effects. In search of new probiotics, 61 lactobacilli isolates were screened for survival in acidic conditions and simulated gastrointestinal juices, tolerance to ox-gall and antimicrobial activity. Selected strains (NWS09, NWS11, NWS14, NWS19 and NWS29) were identified by sequencing their 16S rRNA gene and 16S-23S rRNA gene spacer region, and further characterized by the absence of transferable antibiotic resistance, adhesion to Caco-2 cells and survival in gastrointestinal tract of BALB/c gnotobiotic. NWS09 had transferable resistance to erythromycin conferred by erm(B) gene, while NWS14 was resistant to tetracycline. NWS29, identified as Lactobacillus fermentum showed remarkable tolerance to simulated gastrointestinal juices and bile and highest antimicrobial activity against 5 food borne pathogens by producing heat (100°C) and pH resistant bacteriocins. L. fermentum NWS29 was also found to be highly adhesive to human caco-2 cells in vitro as compared to NWS11 and NWS19 and was isolated in highest numbers from BALB/c gnotobiotic after 24, 48 and 72 h of orogastric inoculation. L. fermentum NWS29 was identified as a probiotic strain that can be incorporated in functional foods for human use.

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Section: Introductionmentioning

confidence: 99%

Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan

Nawaz¹,

Wang²,

Zhou³

et al. 2011

Afr. J. Microbiol. Res.

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“…The results are not unexpected as lactobacilli more often derived from intestinal sources were well adaptable to acid and bile conditions (Kõll et al . ). The suggested reason favored that this strain might have better H+‐ATPase and Bsh activity (Matsumoto et al .…”

Section: Discussionmentioning

confidence: 97%

Evaluation of Survival, Free Radical Scavenging and Human Enterocyte Adherence Potential of Lactobacilli with Anti-Obesity and Anti-Inflammatory CLA Isomer-Producing Attributes

Dahiya

Puniya

2015

Journal of Food Processing and Preservation

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Conjugated linoleic acid (CLA)-producing probiotics are significant in metabolic diseases; therefore, the probiotic-to-functional attributes of six potential antiobesity and anti-inflammatory CLA isomer-producing lactobacilli isolated from infant stools were analyzed. Lactobacillus fermentum DDHI27 and La. plantarum DDHI128 were tolerated well at pH 1.5 with fewer log reductions, whereas in bile, except for La. plantarum DDHI191, all thrived well. Also, these strains have better bile salt deconjugation activity. Higher scavenging activities were noticed for these strains with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radicals compared with 1,1-diphenyl-2-picrylhydrazyl, although DDHI27 and DDHI191 strains were observed as having the most antioxidative activity. Antimicrobial screening revealed that DDHI27 was the most dominating antagonist followed by DDHI128 and DDHI15 strains. These strains were susceptible to most of therapeutic antibiotics except for few and this was correlated with their internal natural phenomenon. Finally, in adhesion assay maximum adherence to Caco-2 cells was noticed with DDHI27. Hence, DDHI27 could be exploited as a source of dietary CLA supplementation. PRACTICAL APPLICATIONSThe screened strain have good probiotic and functional attributes; therefore, it might increase the probiotic diversity in food industry for functional food formulation, especially those that help in managing lifestyle and other diseases. These foods are also the need of the hour, as the majority of available pharmaceutical drugs in market are inefficient and poised adverse effects on the health of the consumer. Additionally, selected strain might be a suitable candidate for gut microbial ecological studies in diseased conditions, as it has shown good antimicrobial and cell adherence ability that might be helpful in replacing the disrupted gut microbiota (responsible for the onset of various metabolic diseases) via competitive exclusion.

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“…The Lactobacillus was categorized on the basis of fermentation group and physiological properties of isolates. The fermentation of glucose without gas, growth at 37°C and no growth at 15°C identifies obligately homofermentative lactobacilli (OHOL); growth both at 15 and 37°C without gas production is characteristic of facultatively heterofermentative lactobacilli (FHEL), whereas gas production at 37°C and variable growth at 15°C are characteristic of obligately heterofermentative lactobacilli (OHEL) (Koll et al, 2010).…”

Section: Phenotypic/physiological Identification Of Isolatesmentioning

confidence: 99%

“…The effect of pancreatin on the survival of lactobacilli was examined by inoculating 1% 24 h old culture in MRS broth containing pancreatin (0.5% w/v) followed by incubation at 37°C and 200 rpm for 24-48 h (Koll et al, 2010). Growth was measured by taking OD after a time interval of 24 and 48 h at 620 nm (Systronics, Japan).…”

Section: Testing Of Pancreatin Tolerancementioning

confidence: 99%

Screening and evaluation of Lactobacillus spp. for the development of potential probiotics

Khagwal¹,

Sharma²,

Sharma³

2014

Afr. J. Microbiol. Res.

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Probiotics are live microbial food ingredients that have beneficial effects on consumption. The present study searched fermented food samples for potential lactobacilli that could be used for the development of novel gastrointestinal probiotics. Fifty five (55) isolates were obtained including 46 bacterial and 9 yeast isolates. On the basis of morphological and biochemical properties, 21 were identified as Lactobacillus spp. Systematic procedures were used to evaluate the probiotic properties of the Lactobacillus isolates including carbohydrate fermentation, autoaggregation, acid tolerance, bile resistance and pancreatin tolerance test. 85% of the examined Lactobacillus spp. showed the ability to form auto-aggregate, 75% can withstand bile while all the strains were able to tolerate pancreatin. Commercially available probiotic strain Lactobacillus casei was isolated and tested for functional criteria required to be a beneficial probiotics and results were compared.

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Screening and Evaluation of Human Intestinal Lactobacilli for the Development of Novel Gastrointestinal Probiotics

Cited by 43 publications

References 30 publications

Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan

Screening and characterization of new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan

Evaluation of Survival, Free Radical Scavenging and Human Enterocyte Adherence Potential of Lactobacilli with Anti-Obesity and Anti-Inflammatory CLA Isomer-Producing Attributes

Screening and evaluation of Lactobacillus spp. for the development of potential probiotics

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