Screening and confirmatory testing of MHC class I alleles in pig-tailed macaques

Fernandez, Caroline S.; Reece, Jeanette C.; Saepuloh, Uus; Rose, Robert De; Ishkandriati, Diah; O’Connor, David H.; Wiseman, Roger W.; Kent, Stephen J.

doi:10.1007/s00251-011-0529-5

Cited by 29 publications

(33 citation statements)

References 33 publications

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“…Future experiments using passive antibody transfer, adoptive T cell transfer, and antibody- mediated depletion of lymphocyte subsets will help to delineate the distinct roles of these cells in mediating cross-protection. Major histocompatibility complex (MHC)-matched primate models have recently been developed that should assist in this work (54,55). Second, we studied a limited number of animals (12 in total), making it difficult to correlate specific immune responses with heightened protection.…”

Section: Discussionmentioning

confidence: 99%

Antibody-Dependent Cellular Cytotoxicity Is Associated with Control of Pandemic H1N1 Influenza Virus Infection of Macaques

Jegaskanda

Weinfurter

Friedrich

et al. 2013

J Virol

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168

166

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Furthermore, we found that influenza virus-specific ADCC was present in bronchoalveolar lavage fluid and was able to activate lung NK cells. We concluded that infection with a seasonal influenza virus can induce antibodies that mediate ADCC capable of recognizing divergent influenza virus strains. Cross-reactive ADCC may provide a mechanism for reducing the severity of divergent influenza virus infections.

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Section: Discussionmentioning

confidence: 99%

Antibody-Dependent Cellular Cytotoxicity Is Associated with Control of Pandemic H1N1 Influenza Virus Infection of Macaques

Jegaskanda

Weinfurter

Friedrich

et al. 2013

J Virol

Self Cite

168

166

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“…Putative Mane-A and Mane-B haplotypes were inferred by identifying combinations of shared alleles between animals as described previously for rhesus macaques (35). MHC-I typing data for 5 out of the 44 macaques have been previously reported (33). The MHC-I typing data are available on request.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting sequencing data were binned by multiplex identifier, and sequence reads from each animal were assembled into unidirectional contigs having 100% identity using a custom genotyping analysis work flow (LabKey, USA). The number of sequence reads comprising each unidirectional contig was enumerated, and the resulting consensus sequences were mapped against an in-house database containing known pig-tailed MHC-I allele sequences using the BLASTn program (33)(34)(35). Putative Mane-A and Mane-B haplotypes were inferred by identifying combinations of shared alleles between animals as described previously for rhesus macaques (35).…”

Section: Methodsmentioning

confidence: 99%

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Linking Pig-Tailed Macaque Major Histocompatibility Complex Class I Haplotypes and Cytotoxic T Lymphocyte Escape Mutations in Simian Immunodeficiency Virus Infection

Gooneratne

Alinejad‐Rokny

Ebrahimi

et al. 2014

J Virol

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The influence of major histocompatibility complex class I (MHC-I) alleles on human immunodeficiency virus (HIV) diversity in humans has been well characterized at the population level. MHC-I alleles likely affect viral diversity in the simian immunodeficiency virus (SIV)-infected pig-tailed macaque (Macaca nemestrina) model, but this is poorly characterized. We studied the evolution of SIV in pig-tailed macaques with a range of MHC-I haplotypes. SIV mac251 genomes were amplified from the plasma of 44 pig-tailed macaques infected with SIV mac251 at 4 to 10 months after infection and characterized by Illumina deep sequencing. MHC-I typing was performed on cellular RNA using Roche/454 pyrosequencing. MHC-I haplotypes and viral sequence polymorphisms at both individual mutations and groups of mutations spanning 10-amino-acid segments were linked using in-house bioinformatics pipelines, since cytotoxic T lymphocyte (CTL) escape can occur at different amino acids within the same epitope in different animals. The approach successfully identified 6 known CTL escape mutations within 3 Mane-A1*084-restricted epitopes. The approach also identified over 70 new SIV polymorphisms linked to a variety of MHC-I haplotypes. Using functional CD8 T cell assays, we confirmed that one of these associations, a Mane-B028 haplotype-linked mutation in Nef, corresponded to a CTL epitope. We also identified mutations associated with the Mane-B017 haplotype that were previously described to be CTL epitopes restricted by Mamu-B*017:01 in rhesus macaques. This detailed study of pig-tailed macaque MHC-I genetics and SIV polymorphisms will enable a refined level of analysis for future vaccine design and strategies for treatment of HIV infection. IMPORTANCECytotoxic T lymphocytes select for virus escape mutants of HIV and SIV, and this limits the effectiveness of vaccines and immunotherapies against these viruses. Patterns of immune escape variants are similar in HIV type 1-infected human subjects that share the same MHC-I genes, but this has not been studied for SIV infection of macaques. By studying SIV sequence diversity in 44 MHC-typed SIV-infected pigtail macaques, we defined over 70 sites within SIV where mutations were common in macaques sharing particular MHC-I genes. Further, pigtail macaques sharing nearly identical MHC-I genes with rhesus macaques responded to the same CTL epitope and forced immune escape. This allows many reagents developed to study rhesus macaques to also be used to study pigtail macaques. Overall, our study defines sites of immune escape in SIV in pigtailed macaques, and this enables a more refined level of analysis of future vaccine design and strategies for treatment of HIV infection.

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“…Mane-A and 22 Mane-B MHC Class I alleles have been identified, and further characterization of the frequency and distribution of at least 10 of these alleles was performed in pigtail macaques of Indonesian, North American, and Australian origin [74,75]. New alleles continue to be identified as the genetic characterization of pigtail macaques progresses, and this is likely to increase their application in vaccine research.…”

Section: Pigtail Macaques (Macaca Nemestrina)mentioning

confidence: 99%

Simian-Human Immunodeficiency Viruses and Their Impact on Non-Human Primate Models for AIDS

Pereira¹,

Srinivasan²,

Smith³

2012

Immunodeficiency

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Screening and confirmatory testing of MHC class I alleles in pig-tailed macaques

Cited by 29 publications

References 33 publications

Antibody-Dependent Cellular Cytotoxicity Is Associated with Control of Pandemic H1N1 Influenza Virus Infection of Macaques

Antibody-Dependent Cellular Cytotoxicity Is Associated with Control of Pandemic H1N1 Influenza Virus Infection of Macaques

Linking Pig-Tailed Macaque Major Histocompatibility Complex Class I Haplotypes and Cytotoxic T Lymphocyte Escape Mutations in Simian Immunodeficiency Virus Infection

Simian-Human Immunodeficiency Viruses and Their Impact on Non-Human Primate Models for AIDS

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