Screening and cloning of multi-drug resistant genes in HL-60/MDR cells

Zheng, Gaihuan; Fu, Jinrong; You-hua, XU; Jin, Xianqing; Liu, Wenli; Zhou, Jianfeng

doi:10.1016/j.leukres.2008.11.018

Cited by 13 publications

(13 citation statements)

References 10 publications

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“…This protein has 4 functional sites related to protein kinase phosphorylation, and its functional domain has homology with the PYRIN domain, an important motif controlling apoptosis. 30,31 Thus, we speculate that HA117 may induce drug resistance by reducing apoptosis of cancer cells.…”

Section: Discussionmentioning

confidence: 96%

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Expression of the Novel All-trans Retinoic Acid-related Resistance Gene HA117 in Pediatric Solid Tumors

Duan

Jin²,

Xiu³

et al. 2014

Journal of Pediatric Hematology/Oncology

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This study aimed to detect the protein expression of HA117 in pediatric solid tumors. Immunohistochemistry was performed to detect the expression of HA117 and P-gp in pediatric solid tumors. In Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL), nephroblastoma (WT), and neuroblastoma (NB), the positive expression rate of HA117 was 65.4%, 58.3%, 81.3%, and 74.1%, and that of P-gp was 57.7%, 70.8%, 65.6%, and 66.7%, respectively. HA117 expression was closely related to the clinical stage of HL (P=0.004) and to the International Prognostic Index score, mediastinal lesions, and clinical stages of NHL (P=0.01, 0.03, and 0.01). The expression of HA117 in WT was higher than in adjacent normal tissues, but there was no statistical significance (P=0.21). The positive expression of HA117 in NB was markedly higher than that in normal tissues (P=0.002), which closely associated with histologic type and lymph node metastasis (P=0.03 and 0.001). Spearman correlation analysis revealed that HA117 expression was not correlated with P-gp in these 4 tumors. This suggests that HA117 might be an important resistance gene in pediatric solid tumors. The mechanism underlying the resistance to all-trans retinoic acid conferred by HA117 is different from that of P-gp.

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Section: Discussionmentioning

confidence: 96%

“…Suppression subtractive hybridization, microarray assays, and sequence homology analysis were used to screen drug resistance-related genes. 2 A novel ATRA-related MDR gene HA117 was identified (GenBank No: AY230154). Previous studies revealed that HA117 is involved in MDR of solid tumors in adults, but its role has not been reported in children tumors.…”

mentioning

confidence: 99%

Expression of the Novel All-trans Retinoic Acid-related Resistance Gene HA117 in Pediatric Solid Tumors

Duan

Jin²,

Xiu³

et al. 2014

Journal of Pediatric Hematology/Oncology

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“…In a previous study, we established a multidrug-resistant cell line (HL60/MDR) to facilitate the search for genes involved in the regulation of tumor cell multidrug resistance (Zheng et al, 2009). With a combination of suppression, subtractive hybridization and microarray methods, we screened and cloned the novel gene HA117 (Genbank accession number AY230154), which was clearly expressed in the drug-resistant HL60/ MDR cell line but not expressed in wild-type HL60 cells.…”

Section: Introductionmentioning

confidence: 99%

Construction, screening and evaluation of a derivative recombinant adenovirus for the optimal siRNA targeting of a novel gene (HA117)

Zheng

Guo

Jin

et al. 2011

Gene

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“…Multidrug resistance-associated protein-1 (MRP1), encoded by the MRP1 gene, is one of the most extensively studied drug transporter. The novel gene HA117 (Gene Bank accession number: AY230154), which was screened and cloned from the all-trans retinoic acid (ATRA)-resistant acute myeloid leukemia cell line HL-60/ATRA using differential hybridization and gene chip assays [1], was shown to be promote MDR in the chronic myelogenous myeloid leukemia cell line K562 [2]. However, the strength and mechanism of the MDR of HA117 have not yet been elucidated, especially in solid tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Multidrug resistance strength of the novel multidrug resistance gene HA117: compared with MRP1

Zhao

Sun

et al. 2010

Med Oncol

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The novel gene HA117 is a multidrug resistance (MDR) gene in all-trans retinoic acid resistance HL-60 cells. The transduction of adenovirus vectors encoding HA117 conferred breast cancer cell line 4T1 MDR not only to MRP1 substrate drugs but also to MRP1 non-substrate drugs and the MDR strength of HA117 was similar to that of multidrug resistance-associated protein-1 (MRP1) for MRP1 substrate, but HA117 had no daunorubicin-excretion function.

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Screening and cloning of multi-drug resistant genes in HL-60/MDR cells

Cited by 13 publications

References 10 publications

Expression of the Novel All-trans Retinoic Acid-related Resistance Gene HA117 in Pediatric Solid Tumors

Expression of the Novel All-trans Retinoic Acid-related Resistance Gene HA117 in Pediatric Solid Tumors

Construction, screening and evaluation of a derivative recombinant adenovirus for the optimal siRNA targeting of a novel gene (HA117)

Multidrug resistance strength of the novel multidrug resistance gene HA117: compared with MRP1

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