Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew

Zhang, Xun; Zhou, Yingying; Li, Yan; Fu, Xinliang; Wang, Qi

doi:10.1016/j.cropro.2017.02.018

Cited by 79 publications

(57 citation statements)

References 31 publications

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“…Cabernet Sauvignon (a wine grape cultivar) in both leaf disk assays and the field. Assays were performed with the culture broth containing both bacterial cells and their metabolites (Zhang et al , ). As B. subtilis is known to produce biologically active molecules, especially CLPs (Ongena and Jacques, ; Stein, ), experiments were performed in order to determine if these molecules were involved in GLB191 activity against P. viticola .…”

Section: Discussionmentioning

confidence: 99%

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Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Héloir

Zhang

et al. 2019

Molecular Plant Pathology

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106

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Summary Bacillus subtilis GLB191 (hereafter GLB191) is an efficient biological control agent against the biotrophic oomycete Plasmopara viticola , the causal agent of grapevine downy mildew. In this study, we show that GLB191 supernatant is also highly active against downy mildew and that the activity results from both direct effect against the pathogen and stimulation of the plant defences (induction of defence gene expression and callose production). High‐performance thin‐layer chromatography analysis revealed the presence of the cyclic lipopeptides fengycin and surfactin in the supernatant. Mutants affected in the production of fengycin and/or surfactin were thus obtained and allowed us to show that both surfactin and fengycin contribute to the double activity of GLB191 supernatant against downy mildew. Altogether, this study suggests that GLB191 supernatant could be used as a new biocontrol product against grapevine downy mildew.

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Section: Discussionmentioning

confidence: 99%

“…However, few have been developed for downy mildew. Recently, we found that the B. subtilis strain GLB191 (hereafter GLB191), isolated from grapevine leaves, is an efficient BCA against downy mildew in both controlled and field conditions (Zhang et al , ). However, the mechanisms of B. subtilis activity against downy mildew are not clear.…”

Section: Introductionmentioning

confidence: 99%

Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Héloir

Zhang

et al. 2019

Molecular Plant Pathology

Self Cite

106

View full text Add to dashboard Cite

show abstract

“…Zhao et al [27] illustrated promising application of endophytic strains of Paenibacillus and Bacillus species from Lonicera japonica in escalating wheat growth and biological control of plant pathogens. Similarly, Zhang et al [28] also highlighted the antagonistic capabilities of endophytic strains of B. pumilus and B. subtilis in protecting grapevine seedlings from downy mildew infection. Later, Haidar et al [29] explored population structure and diversity of bacteria from internal parts of Corchorus olitorius plant and established endophytic B. subtilis as a promising bioinoculant based on its antifungal and PGP attributes.…”

Section: Introductionmentioning

confidence: 99%

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

et al. 2019

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Bacterial endophytes are well known inhabitants of living plant system and perform important assignments in maintaining plant growth and health. Currently, limited reports are available on the endophytes of pearl millet (Pennisetum glaucum) reflecting antagonistic and plant growth promoting (PGP) attributes. Therefore, the major objectives of current investigation were to identify antagonistic strains of endophytic Bacillus from pearl millet and further illustrate their PGP capabilities. In this study, 19 endophytic Bacillus strains (EPP5,

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“…Several resistant varieties were developed [6], but they are still not used for the production of most of the more profitable wines, due to appellation regimes' specifications. Many microorganisms and botanicals were tested as an alternative to synthetic chemical fungicides [7][8][9][10], but most of them have not yet been developed for commercial purposes [5], mainly because of their low and unsteady efficacy in the vineyards.…”

Section: Introductionmentioning

confidence: 99%

Forecasting severe grape downy mildew attacks using machine learning

Chen

Brun²,

Raynal³

et al. 2020

PLoS ONE

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Grape downy mildew (GDM) is a major disease of grapevine that has an impact on both the yields of the vines and the quality of the harvested fruits. The disease is currently controlled by repetitive fungicide treatments throughout the season, especially in the Bordeaux vineyards where the average number of fungicide treatments against GDM was equal to 10.1 in 2013. Reducing the number of treatments is a major issue from both an environmental and a public health point of view. One solution would be to identify vineyards that are likely to be heavily attacked in spring and then apply fungicidal treatments only to these situations. In this perspective, we use here a dataset including 9 years of GDM observations to develop and compare several generalized linear models and machine learning algorithms predicting the probability of high incidence and severity in the Bordeaux region. The algorithms tested use the date of disease onset and/or average monthly temperatures and precipitation as input variables. The accuracy of the tested models and algorithms is assessed by year-byyear cross validation. LASSO, random forest and gradient boosting algorithms show better performance than generalized linear models. The date of onset of the disease has a greater influence on the accuracy of forecasts than weather inputs and, among weather inputs, precipitation has a greater influence than temperature. The best performing algorithm was selected to evaluate the impact of contrasted climate scenarios on GDM risk levels. Results show that risk of GDM at bunch closure decreases with reduced rainfall and increased temperatures in April-May. Our results also show that the use of fungicide treatment decision rules that take into account local characteristics would reduce the number of treatments against GDM in the Bordeaux vineyards compared to current practices by at least 50%.

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Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew

Cited by 79 publications

References 31 publications

Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation

Plant growth promoting and antifungal activity in endophytic Bacillus strains from pearl millet (Pennisetum glaucum)

Forecasting severe grape downy mildew attacks using machine learning

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