Screening and characterization of Bacillus velezensis LB-Y-1 toward selection as a potential probiotic for poultry with multi-enzyme production property

Abstract

Bacillus spp. have gained increasing recognition as an option to use as antimicrobial growth promoters, which are characterized by producing various enzymes and antimicrobial compounds. The present study was undertaken to screen and evaluate a Bacillus strain with the multi-enzyme production property for poultry production. LB-Y-1, screened from the intestines of healthy animals, was revealed to be a Bacillus velezensis by the morphological, biochemical, and molecular characterization. The strain was screened out by a specific screening program, possessed excellent multi-enzyme production potential, including protease, cellulase, and phytase. Moreover, the strain also exhibited amylolytic and lipolytic activity in vitro. The dietary LB-Y-1 supplementation improved growth performance and tibia mineralization in chicken broilers, and increased serum albumin and serum total protein at 21 days of age (p < 0.05). Besides, LB-Y-1 enhanced the activity of serum alkaline phosphatase and digestive enzyme in broilers at 21 and 42 days of age (p < 0.05). Analysis of intestinal microbiota showed that a higher community richness (Chao1 index) and diversity (Shannon index) in the LB-Y-1 supplemented compared with the CON group. PCoA analysis showed that the community composition and structure were distinctly different between the CON and LB-Y-1 group. The beneficial genera such as Parasutterella and Rikenellaceae were abundant, while the opportunistic pathogen such as Escherichia-Shigella were reduced in the LB-Y-1 supplemented group (p < 0.05). Collectively, LB-Y-1 can be considered as a potential strain for further utilization in direct-fed microbial or starter culture for fermentation.

Complete genome analysis of Bacillus velezensis TS5 and its potential as a probiotic strain in mice

“…These findings suggest that Bacillus velezensis TS5 has the potential to promote nutrient digestion. A study indicated that the cellulase and phytase secreted by Bacillus velezensis LB-Y-1 contribute to the release of nutrients in Arbor Acres broiler chickens feed ( Li et al, 2023 ). The protease and amylase secreted by Bacillus velezensis LB-Y-1 enhanced the intestinal digestibility of broilers, thereby promoting nutrient digestion.…”

A New Bacillus velezensis Strain CML532 Improves Chicken Growth Performance and Reduces Intestinal Clostridium perfringens Colonization

“…Fifteen different local breeds of chickens were slaughtered in various regions of China. Broiler cecum was isolated and preserved at −20 • C. Bacillus strain was isolated using the method previously described with minor modifications [12]. The cecal contents were homogenized with phosphate-buffered saline at 1:9 (w/v) ratio, and the compound was then heated in an 80 • C water bath for 10 min.…”

“…B. velezensis ZBG17 isolated from soil samples enhanced feed utilization efficiency and heightened the humoral immune response in broiler chickens [11]. Similarly, B. velezensis LB-Y-1 obtained from the digestive tracts of freeranging animals promoted broiler chickens' growth performance and the mineralization of tibias by enhancing the activities of intestinal digestive enzymes, increasing phosphorus metabolism and utilization, and altering the intestinal microbiota [12]. B. velezensis isolated from piglet manure has been shown to improve the laying hens' egg production and quality, as well as plasma biochemical indicators [13].…”

Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential

“…It is well established that this Bacillus strain has the capacity to generate diverse enzymes, including protease, cellulase, amylase, and glucanase. Additionally, it secretes antibacterial substances, effectively inhibiting the growth of pathogenic microorganisms [64,65]. Gui et al discovered a deep-sea B. velezensis with the ability to break down waterborne polyurethane [66].…”