Screening and Bioprospecting of Anaerobic Consortia for Biofuel Production Enhancement from Sugarcane Bagasse

Soares, Laís Américo; Rabelo, Camila Abreu B. Silva; Sakamoto, Isabel Kimiko; Silva, Edson Luis; Varesche, Maria Bernadete Amâncio

doi:10.1007/s12010-019-03074-2

Cited by 12 publications

(9 citation statements)

References 62 publications

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“…For process intensification, the approaches such as pure culture, continuous operation, consolidated saccharification and fermentation, chemical additions, are optimization avenues. In this work, for convenience of discussions, the comparisons were divided into the following five categories, namely pure culture (PC) [73,[84][85][86][87][88][89][90][91][92][93][94], chemical additions (CA) [2,39,76,[95][96][97], continuous operation with mixed culture (COMC) [98][99][100][101][102], batch operation with mixed culture (BOMC) [103][104][105][106][107][108] and consolidated saccharification and fermentation (CSF) [18,31,61,[109][110][111], respectively. For quantitative comparison of hydrogen yield across different literatures, the necessary Carbon-molar-based mass balance for DF is pivotal and necessary.…”

Section: Results Comparison Among Different Process Intensificationsmentioning

confidence: 99%

A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance

et al. 2020

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This paper reviews the current technological development of bio-hydrogen (BioH2) generation, focusing on using lignocellulosic feedstock via dark fermentation (DF). Using the collected reference reports as the training data set, supervised machine learning via the constructed artificial neuron networks (ANNs) imbedded with feed backward propagation and one cross-out validation approach was deployed to establish correlations between the carbon sources (glucose and xylose) together with the inhibitors (acetate and other inhibitors, such as furfural and aromatic compounds), hydrogen yield (HY), and hydrogen evolution rate (HER) from reported works. Through the statistical analysis, the concentrations variations of glucose (F-value = 0.0027) and acetate (F-value = 0.0028) were found to be statistically significant among the investigated parameters to HY and HER. Manipulating the ratio of glucose to acetate at an optimal range (approximate in 14:1) will effectively improve the BioH2 generation (HY and HER) regardless of microbial strains inoculated. Comparative studies were also carried out on the evolutions of electron equivalent balances using lignocellulosic biomass as substrates for BioH2 production across different reported works. The larger electron sinks in the acetate is found to be appreciably related to the higher HY and HER. To maintain a relative higher level of the BioH2 production, the biosynthesis needs to be kept over 30% in batch cultivation, while the biosynthesis can be kept at a low level (2%) in the continuous operation among the investigated reports. Among available solutions for the enhancement of BioH2 production, the selection of microbial strains with higher capacity in hydrogen productions is still one of the most phenomenal approaches in enhancing BioH2 production. Other process intensifications using continuous operation compounded with synergistic chemical additions could deliver additional enhancement for BioH2 productions during dark fermentation.

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Section: Results Comparison Among Different Process Intensificationsmentioning

confidence: 99%

A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance

et al. 2020

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“…There are many different pretreatments applied to lignocellulose feedstocks to enhance its biodegradability, as it can change the biomass structure and thus also change its adsorptive capacity (Rosero‐Henao et al., 2019). The pretreatments most commonly found in the literature are alkaline hydrogen peroxide and organosolv (Yu et al., 2015), followed by hydrothermal (Lima et al., 2018; Soares et al., 2020), steam explosion (Rabelo et al., 2012; Vivekanand et al., 2012), and dilute acid treatments (Bolado‐Rodríguez et al., 2016). Recently, supercritical and near‐critical carbon dioxide has also been evaluated (Rosero‐Henao et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Methane production from anaerobic digestion of dairy grease trap waste: Effect of sugarcane bagasse addition

Bueno

Henao

Rabelo

et al. 2021

Environmental Quality Mgmt

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Lipids are attractive substrates for anaerobic digestion due to their high methane production yield. However, high concentrations of lipids may cause problems in anaerobic processes. Typically, grease trap waste is mechanically removed and discharged, both generating environmental issues and wasting a potential energy source. In this study, grease trap waste from a dairy wastewater treatment plant was subjected to anaerobic digestion. Untreated and pretreated sugarcane bagasse was evaluated as fat adsorbents to reduce the inhibitory effect of the fatty waste. The bagasse pretreated by the organosolv method was the condition that most contributed to increased methane production (82%) from anaerobic digestion of dairy fatty waste at 0.5 g COD.g–1TVS concentration. Untreated bagasse provided the second‐best condition, followed by hydrothermally treated bagasse, presenting no statistical difference. The obtained results showed the potential of using sugarcane bagasse as a strategy to reduce the inhibitory effect of the fatty waste from the dairy grease trap, making this waste another possible source for generating biogas.

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“…Dysgonomonas have been identified as core microbiota of both higher [45] and lower [46] termites, including Reticulitermes flavipes [47]. There is growing interest in Dysgonomonas due to their prevalence in biotechnological processes such as lignocellulose degradation and bioconversion of polysaccharides for biofuel development [48,49], microbial fuel and electrolysis cells [50][51][52][53][54][55][56][57], wastewater bioreactors [58,59], biodegradation of food waste [60,61] and pharmaceutical compounds [58,[62][63][64].…”

Section: Introductionmentioning

confidence: 99%

Development and application of aerobic, chemically defined media forDysgonomonas

Bridges

Gage

2020

Preprint

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Members of Dysgonomonas are Gram-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation stool and wounds of human patients (CDC group DF-3). More recently Dysgonomonas have been found to be widely distributed in terrestrial environments and are particularly enriched in insect systems. Their prevalence in xylophagous insects such as termites and wood-feeding cockroaches, as well as in soil-fed microbial fuel cells, elicit interest in lignocellulose degradation and biofuel production, respectively. Their prevalence in mosquito and fruit fly have implications relating to symbiosis, host immunology and developmental biology. Additionally, their prevalence in termite, mosquito and nematode present novel opportunities for pest and vector control. Currently, the absolute growth requirements of Dysgonomonas are unknown, and they are cultured solely under anaerobic conditions on complex media containing blood, peptones, tryptones, and yeast, plant or meat extracts. Restrictive & undefined culturing conditions preclude physiological and genetic studies, and thus further understanding of metabolic potential. Here we describe the requirements for growth of termite-derived Dysgonomonas isolates and create parallel defined, minimal and complex media that permit vigorous and reliable aerobic growth. Furthermore, we show that these media can be used to easily enrich for Dysgonomonas isolates from complex and microbially-diverse environmental samples.Impact StatementMembers of the genus Dysgonomonas are increasingly prevalent in ecological, medical and biotechnological contexts. To the best of our knowledge, there are currently no formulations for chemically defined or minimal media for Dysgonomonas, and limited complex formulations that allow aerobic growth, particularly on solid media. We have created three parallel media formulations (complex, defined & minimal) that permit robust aerobic and anaerobic growth in liquid and agar-solidified media. These formulations remove the necessity for animal blood and expensive equipment such as anaerobic chambers, which can inhibit basic research by groups with biosafety and resource limitations.

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Screening and Bioprospecting of Anaerobic Consortia for Biofuel Production Enhancement from Sugarcane Bagasse

Cited by 12 publications

References 62 publications

A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance

A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance

Methane production from anaerobic digestion of dairy grease trap waste: Effect of sugarcane bagasse addition

Development and application of aerobic, chemically defined media forDysgonomonas

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