Screening and analysis on the protein interaction of the protein VP7 in grass carp reovirus

Abstract

Grass carp reovirus (GCRV) has caused serious economic losses for several decades in China. The protein VP7 is one of the important structural proteins in GCRV. Recent studies indicated that the protein VP7 had the commendable antigenicity and immunogenicity. The protein VP7 cooperated with VP5 could change the conformation of the cell membrane and facilitate entry of GCRV into host cells. We speculated that the protein VP7 should play an important role in the pathogenesis of GCRV. In order to explore the function of the protein VP7, the bait protein expression plasmid pGBKT7-vp7 and the cDNA library of CIK cells were constructed. By yeast two-hybrid system, after multiple screening with the high screening rate medium, rotary verification, sequencing and bioinformatics analysis, the interactions of the protein VP7 with ribosomal protein S20 (RPS20) and eukaryotic translation initiation factor 3 subunit b (eIF3b) in CIK cells were identified. RPS20 played the important roles in the generation of influenza B virus and a variety of diseases. eIF3b was relative to the infection of some viruses. This study suggested that the protein VP7 played the role in viral replication and most likely interacted with host proteins by RPS20 and eIF3b. The interaction mechanisms of the protein VP7 with RPS20 and eIF3b, and the subsequent effector mechanisms needed to be further studied. The corresponding protein interaction of the protein VP7 was not acquired in bioinformatics. The protein VP7 and its untranslated region may have the unknown special function. This study laid the foundation for deeply exploring the function of the protein VP7 in GCRV and had the important scientific significance for exploring the pathogenic mechanism of GCRV.

Prediction of GCRV virus-host protein interactome based on structural motif-domain interactions

“…Recently, the potential functional roles of interactions mediated by motifs and their counterpart domains in viral infection have been addressed in a number of recent articles [13, 16], demonstrating the power of motif-based approach. For GCRV and its host grass carp, heretofore, there are few published reports about their PPIs, only the PPIs targeted by VP7 protein in GCRV GD873 were screened by using yeast two-hybrid system [17]. Hence there is an urgent need to study the GCRV virus-host interactome systematically, which may help us to understand the underlying pathogenesis of GCRV infection.…”