Screening and 16S rRNA Analysis of the Bacteria of Producing Chitin Deacetylase

Zhou, Guoying; He, Yong; Huai-yun, Zhang

doi:10.1109/icbbe.2010.5517350

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…This finding suggests that a correlation exists between different antibiotic resistance levels. Meanwhile, deacetylases classified as CE3, CE4, CE7, CE8, and CE11 deserve our attention due to their roles in the hydrolysis of acetamides [ 44 ], which may be involved in tetracycline degradation via deamination. Furthermore, 171 unknown function genes were upregulated in our study and may be involved in tetracycline degradation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Pei

Sun

Zhang

et al. 2023

Toxics

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Black soldier fly (Hermetia illucens L) larvae (BSFL) possess remarkable antibiotic degradation abilities due to their robust intestinal microbiota. However, the response mechanism of BSFL intestinal microbes to the high concentration of antibiotic stress remains unclear. In this study, we investigated the shift in BSFL gut microbiome and the functional genes that respond to 1250 mg/kg of tetracycline via metagenomic and metatranscriptomic analysis, respectively. The bio-physiological phenotypes showed that the survival rate of BSFL was not affected by tetracycline, while the biomass and substrate consumption of BSFL was slightly reduced. Natural BSFL achieved a 20% higher tetracycline degradation rate than the germ-free BSFL after 8 days of rearing. Metagenomic and metatranscriptomic sequencing results revealed the differences between the entire and active microbiome. Metatranscriptomic analysis indicated that Enterococcus, Vagococcus, Providencia, and Paenalcaligenes were the active genera that responded to tetracycline. Furthermore, based on the active functional genes that responded to tetracycline pressure, the response mechanisms of BSFL intestinal microbes were speculated as follows: the Tet family that mediates the expression of efflux pumps expel tetracycline out of the microbes, while tetM and tetW release it from the ribosome. Eventually, tetracycline was degraded by deacetylases and novel enzymes. Overall, this study provides novel insights about the active intestinal microbes and their functional genes in insects responding to the high concentration of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Pei

Sun

Zhang

et al. 2023

Toxics

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“…These tubes were then incubated at 25°C for 12–24 hours. After incubation, the development of yellow color in the strip indicates the presence of deacetylase in respective bacterial isolate [ 11 – 13 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Chitosan-Producing Bacteria from Beaches of Chennai, India

et al. 2012

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Chitosan is a deacetylated product of chitin produced by chitin deacetylase, an enzyme that hydrolyses acetamido groups of N-acetylglucosamine in chitin. Chitosan is a natural polymer that has great potential in biotechnology and in the biomedical and pharmaceutical industries. Commercially, it is produced from chitin via a harsh thermochemical process that shares most of the disadvantages of a multistep chemical procedure. It is environmentally unsafe and not easily controlled, leading to a broad and heterogeneous range of products. An alternative or complementary procedure exploiting the enzymatic deacetylation of chitin could potentially be employed, especially when a controlled and well-defined process is required. In this study, 20 strains of bacteria were isolated from soil samples collected from different beaches of Chennai, India. Of these 20 bacterial strains, only 2 strains (S3, S14) are potent degrader of chitin and they are also a good producer of the enzyme chitin deacetylase so as to release chitosan.

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Screening and 16S rRNA Analysis of the Bacteria of Producing Chitin Deacetylase

Cited by 2 publications

References 3 publications

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Isolation and Characterization of Chitosan-Producing Bacteria from Beaches of Chennai, India

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