Screening, analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong

Liang, Ming-Jin; He, Langchong; Yang, Guangde

doi:10.1016/j.lfs.2005.04.038

Cited by 117 publications

(70 citation statements)

References 27 publications

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“…It has antiapoptotic effect associated with down-regulating the expressions of Bcl-2 and Bcl-2 Associated X protein (bax) along with suppressing of caspase-3 (Chang et al, 2007). It is widely used in China to treat neurovascular disorders, ischemic stroke, pulmonary hypertension and obstructive pulmonary diseases due to its effectiveness and low toxicity (Hintz and Ren, 2003;Liang et al, 2005). In present study, we established an animal model to assess the effects of TMP for the treatment and control of TD in broiler chickens and its effect on liver antioxidant capabilities.…”

Section: Research Articlementioning

confidence: 99%

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Mehmood¹

2018

PVJ

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Section: Research Articlementioning

confidence: 99%

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Mehmood¹

2018

PVJ

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“…L. wallichii is commonly used to treat cardiovascular diseases, 4,10,21) and some organic acids, including ferulic acid, sedanonic acid, folic acid, vanillic acid, and caffeic acid, have been purified from L. wallichii. 22,23) Additionally, Liang et al 24) reported that ligustilide and butylidenephthalide from L. wallichii significantly inhibited the vasoconstriction induced by NE and calcium chloride. Ko et al 25) also reported that butylidenephthalide exerted a significant antihypertensive effect in hypertensive rats.…”

Section: Discussionmentioning

confidence: 99%

Endothelium-Independent Vasorelaxation by Ligusticum wallichii in Isolated Rat Aorta: Comparison of a Butanolic Fraction and Tetramethylpyrazine, the Main Active Component of Ligusticum wallichii

Kim

Rhyu

2010

Biological & Pharmaceutical Bulletin

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Ligusticum wallichii FRANCHAT (or Ligusticum chuanxiong hort; L. wallichii), a member of the Umbelliferaceae family, is a Chinese medicinal herb that is a common constituent in polypharmaceutical herbal drugs. It has been widely used in Asian countries to increase coronary blood flow and systemic circulation, and to relieve stasis. 1,2) Previous studies have indicated that L. wallichii improves blood fluidity 3) and inhibits endothelial cell damage 4) and vascular smooth muscle cell proliferation. 5)We demonstrated that an extract of L. wallichii showed endothelium-dependent acute relaxation in isolated rat thoracic aorta.6) The effect of L. wallichii was due to endothelial nitric oxide (NO). However, the tetramethylpyrazine (TMP)-induced vasorelaxant effect was not related to endothelial NO. TMP was isolated as a biologically active component from L. wallichii. Since 1970, TMP has been used in China for the treatment of patients with angina pectoris and cerebral ischemic syndromes. [7][8][9] Earlier pharmacological studies demonstrated that aortic vasorelaxation induced by TMP involved the opening of ATP-sensitive K ϩ channels and/or small-conductance Ca 2ϩ -activated K ϩ channels. [10][11][12] Endothelium-derived relaxing factor (EDRF) and the membrane potential are major components in modulating vascular tone.13) After EDRF, the most important molecule is probably NO,14,15) and K ϩ channels play an important role in the regulation of membrane potential.16) Five distinct types of K ϩ channel have been documented in arterial smooth muscle: voltage-dependent K ϩ (K V ) channels, large-conductance Ca 2ϩ -activated K ϩ (LK Ca ) channels, small-conductance Ca 2ϩ -activated K ϩ (SK Ca ) channels, inward rectifier K ϩ (K IR ) channels, and ATP-sensitive K ϩ (K ATP ) channels. 16) Additionally, changes in K ϩ channel activity to produce a subsequent alteration in the activity of calcium channels may result in vasorelaxation.17) Thus, the present study was performed to separate the active fraction, to examine the active fractioninduced changes in membrane potential, the possible underlying mechanisms, and to perform comparisons with TMP using isolated endothelium-denuded rat aortic rings. MATERIALS AND METHODS Extraction and FractionationDried, sliced L. wallichii was obtained from a local market and ground with a commercial food mixer. The resulting powder was extracted under reflux with absolute ethanol for 1 h. The extract was evaporated under reduced pressure at low temperature (37-40°C) and lyophilized (LwEx). Extracts for all of the experiments were prepared from four batches of L. wallichii to assess variation in individual extracts. LwEx was fractionated successively with water and n-butanol, and each phase was concentrated and lyophilized into water-soluble (LwDw) and butanol-soluble (LwBt) fractions. The solid was stored at Ϫ20°C until use. LwEx and LwBt were dissolved in 30% ethanol and LwDw was dissolved in water to give final concentrations of 0.3 to 3.0 mg/ml in the bath. Voucher specimen nos. Lw-001 (LwE...

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“…Previous studies have indicated the possible link between the plant and heart disease. L. wallichii could improve blood fluidity (Yan and Luo, 2002) and inhibit endothelial cell damage (Wang et al, 2013) and vascular smooth muscle cell proliferation (Liang et al, 2005). Several studies demonstrated that L. wallichii could be used to treat headache, pregnancy pain, post-natal bruising, new born pain, wind attack, migraines, schwindlig, pregnancy, postpartum abdominal pain and pediatric head pain (Ozawa et al, 1999;Sugino et al, 2000;Wu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Liguisticum wallichii inhibits renal carcinoma progression by downregulating UBE3A and through suppression of NF-κB signaling

Zhang

Sun³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Renal carcinoma accounts for a fifth of the morbidity among malignant tumors in China. Ubiquitin-protein ligase E3A (UBE3A) plays an important role in the occurrence and development of gene mutation-induced diseases. This study was designed to investigate the mechanism of Liguistium wallichii in treating renal carcinoma. Hematoxylin and eosin staining was applied to detect the pathological changes in a rat renal carcinoma model. The experimental group received L. wallichii treatment at 100 mg/kg every 48 h for 4 weeks, while the control group only received normal saline. The proliferation index Ki67 was measured by immunohistochemistry. Primary renal carcinoma cells were isolated and UBE3A expression was measured by quantitative polymerase chain reaction. The related signaling pathway was screened by the Pathway Finder Array. pP65 nuclear import was detected by immunofluorescence. A total of 60 rats were used for the renal carcinoma model, of which 58 rats were successfully established and equally divided into two groups: L. wallichii and normal saline. Ki67 expression decreased in the L. wallichii group and was upregulated in the normal saline group. Histological analysis showed significant renal cell nucleus division in the normal saline group. The UBE3A level decreased after L. wallichii treatment compared to the level in the normal saline group. The Pathway Finder Array revealed that the NF-κB signaling pathway was activated, and pP65 presented obvious nuclear import in the normal saline group. In conclusion, L. wallichii inhibits renal carcinoma progression by downregulating UBE3A and suppressing the NF-κB signaling pathway.

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Screening, analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong

Cited by 117 publications

References 27 publications

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Endothelium-Independent Vasorelaxation by Ligusticum wallichii in Isolated Rat Aorta: Comparison of a Butanolic Fraction and Tetramethylpyrazine, the Main Active Component of Ligusticum wallichii

Liguisticum wallichii inhibits renal carcinoma progression by downregulating UBE3A and through suppression of NF-κB signaling

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