Screening active components from Rubus amabilis for pancreatic β-cells protection

Abstract

Context: Rubus species (Rosaceae) have been used in folk medicine to treat diabetes due to their hypoglycaemic activity. Objective: To screen the active components that act as hypoglycaemic agents in Rubus amabilis Focke and the underlying mechanisms. Materials and methods: Aqueous stem extract of R. amabilis was incubated with MIN6 b-cells, PBS was used as the blank control. Then the cells were washed, cell membrane-bound components were dissociated and identified by UPLC/MS. Total procyanidins (PCs) in R. amabilis was enriched and the cytotoxicity and anti-proliferation on b-cell were evaluated by MTT assay. PCs at 25, 50, and 75 lg/mL was applied for 24 h to determine its effects on palmitate (PA)-induced apoptosis and GSIS. Western blotting was employed to detect the protein expression of PI3K/Akt/FoxO1 signalling. The antioxidant indices were also measured. Results: b-Cell membrane-bound components were identified as three procyanidin B dimers and a C trimer. PCs showed no significant cytotoxicity up to a concentrations of 100 lg/mL. PCs treatment reversed the elevated apoptosis rate and impaired GSIS induced by PA. PCs markedly decreased the intracellular ROS and MDA production and increased the SOD activity. Moreover, PCs promoted the phosphorylation of Akt and FoxO1, and regulated Pdx-1 and Bax expression in MIN6 cells. Discussion and conclusion: The active components that act as hypoglycaemic agents in R. amabilis are procyanidins, which protected MIN6 cells against PA-induced apoptosis by activating PI3K/Akt/FoxO1 signalling. These results indicate that b-cell extraction, combined with UPLC/MS, is a valid method for screening antidiabetic components from herbal medicines.

Liraglutide protects palmitate-induced INS-1 cell injury by enhancing autophagy mediated via FoxO1

“…There are certain limitations to the present study. First, PA was used to test the cell viability, apoptosis and autophagy of pancreatic β-cells, based on its lipotoxicity ( 40 , 41 ). Previous studies also proposed that PA could participate in apoptosis via reactive oxygen species and ERs generation in isolated rat islets of Langerhans ( 28 , 29 , 37 , 42 ).…”

Radical oxygen species: an important breakthrough point for botanical drugs to regulate oxidative stress and treat the disorder of glycolipid metabolism

“…Trigonella foenum-graecum seeds restored dexamethasone-induced glucose uptake in IR3T3-L1 cells by activating the AKT and AMPK (Luan et al, 2018). Rubus amabilis active ingredients treat MINI6β cells to regulate the activation of the AKT/FoxO1 pathway and play an antiapoptotic role (Sun et al, 2020). Epimedium can reduce mitochondrial autophagy of cavernous smooth muscle cells through PI3K/AKT/ mTOR pathway and improve glucose metabolism and ROS production in T2DM rats with erectile dysfunction (Zhang et al, 2020).…”

Procyanidins: A promising anti‐diabetic agent with potential benefits on glucose metabolism and diabetes complications

“…These outcomes demonstrate that β-cells extraction in conjunction with UPLC/MS is a reliable technique for analysing antidiabetic ingredients in herbal remedies. 92 One of the A-type PC oligomers derived from Cinnamomum tamala, cinnamtannin D1 (CD-1), was shown recently to have the ability to prevent apoptosis in pancreatic cells that had been exposed to palmitic acid by reducing OS in vitro.…”