Screen-Printed Voltammetric Biosensors for the Determination of Copper in Wine

Abstract

Certain heavy metals present in wine, including copper, can form insoluble salts and can induce additional casse, so their determination is important for its quality and stability. In this context, a new biosensor for quantification of copper ions with BSA protein (bovine serum albumin) and using SPE electrodes (screen-printed electrodes) is proposed. The objective of this research was to develop a miniaturized, portable, and low-cost alternative to classical methods. A potentiostat, which displays the response in the form of a cyclic voltammogram, was used in order to carry out this method. Values measured for the performance characteristics of the new biosensor revealed a good sensitivity (21.01 μA mM−1cm−2), reproducibility (93.8%), and limit of detection (0.173 ppm), suggesting that it has a high degree of application in the analysis proposed by our research. The results obtained for wine samples were compared with the reference method, atomic absorption spectrometer (AAS), and it was indicated that the developed biosensor is efficient and can be used successfully in the analysis of copper in wine. For the 20 samples of red wine analyzed with AAS, the concentration range of copper was between 0.011 and 0.695 mg/L and with the developed biosensor it was between 0.037 and 0.658 mg/L. Similar results were obtained for the 20 samples of white wine, 0.121–0.765 mg/L (AAS) and 0.192–0.789 mg/L (developed biosensor), respectively.

Development, Optimization, Characterization, and Application of Electrochemical Biosensors for Detecting Nickel Ions in Food

“…The results were obtained by cyclic and linear sweep voltammetry. Sensitivity was calculated using Equation (1) below: Sensitivity = m/A where m is the slope of the calibration curve (µA Mm −1 ), and A is the area of active surface (cm 2 ) [ 56 ].…”

Biopolymers Used for Receptor Immobilization for Nickel-Detection Biosensors in Food

“…Sensitivity was calculated using the basic formula below (Equation (1)): Sensitivity = m/A where m—slope of calibration curve (μA mM −1 ); A—area of active surface (cm 2 ) [ 37 , 38 ].…”

An overview of Structured Biosensors for Metal Ions Determination

“…The association of this strategy with electrochemical transducers significantly lowered the detection limits, especially when stripping voltammetry was employed for the determination of elements [17,46,47]. The coordination of metal ions with proteins [74], aminoacids [17,[75][76][77][78][79], synthetized peptides [70], hormones (melatonine [80] and oxytocin [7]), DNA strands [17,46,[81][82][83], and a neurotransmitter (neurokinin B [38]) were exploited to achieve detection limits as low as 0.80 ag L −1 [81]. The selected articles regarding this mechanism are displayed in Table 1.…”