Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Abstract

Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

Biosensor Arrays for Environmental Monitoring

“…Lower limits of detection for pesticides were achieved in the steady state system: carbaryl 26 nmol/L, heptenophos 14 nmol/L and fenitrothion 0.58 nmol/L. Similar multi-enzyme-based electrochemical biosensor arrays for the determination of pesticides [49][50][51][52] and phenols [53] have been reported by other workers.…”

Electrochemical Arrays for Bioassay Applications

“…The characterization of wastewater samples was the objective of a study of small screen-printed amperometric enzyme biosensor arrays [34]. This study considered The potential difference at the electrode/solution interface will vary across the length of the electrode according to the potential gradient applied to the solution.…”

Electroanalysis Utilizing Amperometric Microdisk Electrode Arrays

“…The main problem is that as environmental waters are complex and heterogeneous, biosensors generate biased responses due to the presence of various interfering compounds. These issues may be overcome by designing multibiosensors consisting of several transducing elements containing bioselective components specific to different substances, e.g., a multienzyme biosensor array based on immobilization of enzymes from different natural sources (tyrosinase, peroxidases, cellobiose dehydrogenase and cholinesterase) for phenols and pesticides detection [134,135]. Fig.…”