Screen-printed electrodes with electropolymerized Meldola Blue as versatile detectors in biosensors

Vasilescu, Alina; Andreescu, Silvana; Bala, Camelia; Liţescu, Simona Carmen; Noguer, Thierry; Marty, Jean Louis

doi:10.1016/s0956-5663(03)00044-7

Cited by 74 publications

(41 citation statements)

References 31 publications

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“…Another strategy is modification of the electrode surface with a polymeric substance, using electrodes modified with carbon nanotubes, nanofibers or using enzymatic methods that follow bioelectrocatalytic reaction [2,4,13,15,16,[20][21][22][23]. Investigation in this area usually explores the mediator use or the surface modification to improve the electrochemical detection [15,21,[24][25][26][27][28]. Some literature has been published related to the study of the redox couple NADH/NAD + in screen printing electrodes [1,4,21,[24][25][26][27][29][30][31][32][33][34].…”

Section: David Publishingmentioning

confidence: 99%

“…Investigation in this area usually explores the mediator use or the surface modification to improve the electrochemical detection [15,21,[24][25][26][27][28]. Some literature has been published related to the study of the redox couple NADH/NAD + in screen printing electrodes [1,4,21,[24][25][26][27][29][30][31][32][33][34]. The sensor built using this technique can incorporate the three electrodes (working electrode, reference and counter electrode), can be easily produced and miniaturized, work with a minimum volume (2 µL), it needs low reagent consumption, it can be fabricated in many supports (paper, glass) and sensors are disposable devices that can be used in many science fields [26,35].…”

Section: David Publishingmentioning

confidence: 99%

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A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

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Abstract:A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 µL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD + redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD + signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD + /NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).

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Section: David Publishingmentioning

confidence: 99%

Section: David Publishingmentioning

confidence: 99%

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

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“…Para o procedimento 1, foi preparada uma pasta enzimática contendo 30% (v/v) de PVA-SbQ e 70% (v/v) da solução de AldH. A atividade enzimática, na solução utilizada para preparo da pasta, foi previamente determinada por espectrofotometria 25,32 . Uma porção de 2 μL dessa mistura foi depositada manualmente na superfície do eletrodo de trabalho.…”

Section: Imobilização Da Enzima Aidhunclassified

Biossensor enzimático para detecção de fungicidas ditiocarbamatos: estudo cinético da enzima aldeído desidrogenase e otimização do biossensor

Lima¹,

Nunes²,

Noguer³

et al. 2007

Quím. Nova

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Recebido em 13/7/05; aceito em 3/4/06; publicado na web em 11/8/06 ENZYMATIC BIOSENSOR FOR THE DETECTION OF DITHIOCARBAMATE FUNGICIDES. KINETIC STUDY OF ALDEHYDE DEHYDROGENASE ENZYME AND BIOSENSOR OPTIMIZATION. Initially, all major factors that affect the rate of the AldH-catalyzed reaction (enzyme concentration, substrate concentration, temperature and pH) were investigated. Optimal activity was observed between pH values of 7.5 and 9.5 in the temperature range of 25 to 50 o C. Kinetic parameters, such as K m (2.92 μmol L -1 ) and V max (1.33 10 -2 μmol min -1 ) demonstrate a strong enzyme-substrate affinity. The sensors were based on screen-printed electrodes modified with the Meldola BlueReinecke salt (MBRS) combination. Operational conditions (NAD + and substrate contents, enzyme loading and response time) were optimized. Also, two enzyme immobilization procedures were tested: entrapment in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) and crosslinking with glutaraldehyde. Chronoamperometry was employed to observe the biosensor responses during enzymatic hydrolysis of propionaldehyde and also to construct inhibition curves with maneb and zineb fungicides. Best results were found with the following conditions:; enzyme loading = 0.8 U per electrode; response time = 10 min, and inhibition time = 10 min. Current intensities around 103 ± 13 nA with the sensors and good stability was obtained for both immobilization procedures. Detection limits, calculated using 10% inhibition were 31.5 μg L -1 and 35 μg L -1 for maneb and zineb, respectively. Results obtained with other MBRS-modified electrodes consisting of mono and bi-enzymic sensors were compared. The ability to catalyze NADH oxidation by MB was also highlighted.Keywords: AldH-based biosensors; dithiocarbamate fungicides; enzymatic kinetic. INTRODUÇÃOA investigação dos pesticidas constitui um caso delicado. A necessidade de analisar resíduos de pesticidas depara-se com problemas analíticos complexos, devido à gama de substâncias existentes em classes distintas e que devem ser determinadas em diferentes tipos de matrizes, sejam elas ambientais ou de alimentos 1 . Entre os agrotóxicos mais difundidos atualmente, encontram-se os fungicidas ditiocarbamatos (DTC's). Tais compostos formam a mais importante classe de pesticidas empregados no controle de diversas doenças fungais em sementes, frutos e vegetais. Embora a toxidade aguda dos DTC's seja relativamente baixa, existe uma urgente necessidade de se encontrar um método de detecção sensível, confiável, e que permita rápidas tomadas de decisão, no caso de intoxicação em trabalhadores rurais e/ou contaminação ambiental ou alimentar. Isto devido ao fato de que, dependendo dos compostos encontrados, estes podem vir a ser carcinogênicos 2 , goitrogênicos 2 , mutagênicos 3-5 , teratogênicos 3,6,7 , provocar distúrbios neurológicos 8 e, também, afetar a biota aquática 9 . Há uma grande preocupação com relação a certos compostos como, por ex., os fungicidas maneb e ditane, que contêm manganês, precursor d...

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“…A series of water soluble organic dyes such as methylene green [18], meldola blue [19], Prussian blue [20] and celestine blue [21] have been used for the modification of electrode surface and they exhibit excellent mediating ability for the electrocatalytic reduction of H 2 O 2 . We used brilliant cresyl blue (BCB) dye in our investigation due to its higher sensitivity and mediating ability toward H 2 O 2 determination.…”

Section: Introductionmentioning

confidence: 99%

Analytical Biosensing of Hydrogen Peroxide on Brilliant Cresyl Blue/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

Peng¹,

Upadhyay²,

Chen³

2010

Electroanalysis

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A cationic quinine-imide dye brilliant cresyl blue (BCB) and horseradish peroxidase (HRP) were co-immobilized within ormosil on multiwalled carbon nanotubes modified glassy carbon electrode for the fabrication of highly sensitive and selective hydrogen peroxide biosensor. The presence of epoxy group in ormosil as organic moiety improves the mechanical strength and transparency of the film and amino group provides biocompatible microenvironment for the immobilization of enzyme. The presence of MWCNTs improved the conductivity of the nanocomposite film. The surface characterization of MWCNT modified ormosil nanocomposite film was performed with scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cyclic voltammetry and amperometry measurements were used to study and optimize the performance of the resulting peroxide biosensor. The apparent Michaelis -Menten constant was determined to be 1.5 mM. The proposed H 2 O 2 biosensor exhibited wide linear range from 3 Â 10 À7 to 1 Â 10 À4 M, and low detection limit 1 Â 10 À7 M (S/N ¼ 3) with fast response time < 5 s. The probable interferences in bio-matrix were selected to test the selectivity and no significant response was observed in the biosensor. This biosensor possessed good analytical performance and long term storage stability.

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Screen-printed electrodes with electropolymerized Meldola Blue as versatile detectors in biosensors

Cited by 74 publications

References 31 publications

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Biossensor enzimático para detecção de fungicidas ditiocarbamatos: estudo cinético da enzima aldeído desidrogenase e otimização do biossensor

Analytical Biosensing of Hydrogen Peroxide on Brilliant Cresyl Blue/Multiwalled Carbon Nanotubes Modified Glassy Carbon Electrode

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