Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Mincu, Nicolae-Bogdan; Lazăr, Veronica; Stan, Dana; Mihailescu, Carmen-Marinela; Iosub, Rodica; Mateescu, Andreea Lorena

doi:10.3390/diagnostics10080517

Cited by 58 publications

(42 citation statements)

References 67 publications

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“…Due to the high reproducibility between electrodes, these sensors are suitable for a wide range of applications: clinical sensing platforms development and environmental and food industry analysis. Moreover, sensor arrays allow the determination of multiple substances in parallel [26].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

Munteanu

Apetrei

2021

IJMS

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The present study describes the electrochemical properties of three screen-printed electrodes (SPEs), the first electrode being carbon-based (C), the second graphene-based (GPH), and the third based on GPH modified with gold nanoparticles (GNP). These electrodes were used for the study of the electrochemical behavior of chlorogenic acid in different aqueous solutions, at pH = 7. In chlorogenic acid solution, a redox process was noticed in the case of all three electrodes; GPH and GNP significantly improved the sensor response regarding sensitivity and reversibility, a fact demonstrated by characterizing the sensor by cyclic voltammetry in potassium ferrocyanide, which corresponds to the exchange of two electrons and two protons. Moreover, the calibration curves for each sensor were developed, subsequently calculating the detection limits (LOD) and the quantification limits (LOQ). Low LOD and LOQ were obtained, the best—of the order of 10−7 M (LOD = 0.62 × 10−7 M; LOQ = 1.97 × 10−7 M)—being obtained in the case of GPH-GNP-SPE, which demonstrates that the method may be applied for determining chlorogenic acid in real samples. Thus, the sensors were successfully used for the quantitative determination of chlorogenic acid in three nutraceutical products. The validation of the results was done using the FTIR method. The results obtained by cyclic voltammetry were in accordance with those obtained by the spectrometric method, without significant differences from a statistical point of view.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

Munteanu

Apetrei

2021

IJMS

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“…Currently, screen-printed electrodes (SPEs) seem to be the preferred option to develop fast and cost-effective in vitro diagnosis methods with a large variety of applications [ 69 ], due to the fact they can be mass-produced from a variety of materials and in a customable way (different geometries, multiplexed formats, nanoparticulated and with suitable tailor-made functionalities) [ 70 ]. In addition, their small dimensions allow the use of small sample volumes and their flat shape facilitates the incorporation of magnetic bioconjugates on their surface in a stable and reproducible way by simple magnetic attraction.…”

Section: Bioelectroanalytical Methods For the Determination Of Infmentioning

confidence: 99%

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

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“…SPEs can be manufactured on various substrates such as plastic, paper, tattoo, etc., and they are characterized by high versatility in selecting the size, geometry, dimensionality, and customization methods [ [18] , [19] , [20] , [21] , [22] ]. The use of screen-printed electrodes (SPEs) could be of the highest interest to develop new methods and research.…”

Section: Introductionmentioning

confidence: 99%

Nano-engineered screen-printed electrodes: A dynamic tool for detection of viruses

Sher

Faheem

Asghar

et al. 2021

TrAC Trends in Analytical Chemistry

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There is a growing interest in the development of portable, cost-effective, and easy-to-use biosensors for the rapid detection of diseases caused by infectious viruses: COVID-19 pandemic has highlighted the central role of diagnostics in response to global outbreaks. Among all the existing technologies, screen-printed electrodes (SPEs) represent a valuable technology for the detection of various viral pathogens. During the last five years, various nanomaterials have been utilized to modify SPEs to achieve convincing effects on the analytical performances of portable SPE-based diagnostics. Herein we would like to provide the readers a comprehensive investigation about the recent combination between SPEs and various nanomaterials for detecting viral pathogens. Manufacturing methods and features advances are critically discussed in the context of early-stage detection of diseases caused by HIV-1, HBV, HCV, Zika, Dengue, and Sars-CoV-2. A detailed table is reported to easily guide readers toward the “right” choice depending on the virus of interest.

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Screen-Printed Electrodes (SPE) for In Vitro Diagnostic Purpose

Cited by 58 publications

References 67 publications

Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Nano-engineered screen-printed electrodes: A dynamic tool for detection of viruses

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