Screen-Printed Electrodes Modified with Metal Nanoparticles for Small Molecule Sensing

Antuña-Jiménez, Daniel; González‐García, María Begoña; Hernández‐Santos, David; Fanjul‐Bolado, Pablo

doi:10.3390/bios10020009

Cited by 107 publications

(60 citation statements)

References 98 publications

(198 reference statements)

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“…screen-printed electrodes (SPEs), have attracted a huge attention for the miniaturization of both the electrochemical sensor as well as the reader ( Caratelli et al, 2020 ), the sensitivity, the capability to work in complex matrices, and the easiness to use ( Arduini et al, 2016 ). In addition, SPEs are easily modified with nanomaterials namely gold nanoparticles, graphene, and carbon black during the mass-production to improve the electroanalytical performances and increasing the sensitivity of biosensors ( Antuña-Jiménez et al, 2020 ; Arduini et al, 2020 ; Cinti and Arduini, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

Fabiani

Saroglia

Galatà³

et al. 2021

Biosensors and Bioelectronics

353

316

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The diffusion of novel SARS-CoV-2 coronavirus over the world generated COVID-19 pandemic event as reported by World Health Organization on March 2020. The huge issue is the high infectivity and the absence of vaccine and customised drugs allowing for hard management of this outbreak, thus a rapid and on site analysis is a need to contain the spread of COVID-19. Herein, we developed an electrochemical immunoassay for rapid and smart detection of SARS-CoV-2 coronavirus in saliva. The electrochemical assay was conceived for Spike (S) protein or Nucleocapsid (N) protein detection using magnetic beads as support of immunological chain and secondary antibody with alkaline phosphatase as immunological label. The enzymatic by-product 1-naphtol was detected using screen-printed electrodes modified with carbon black nanomaterial. The analytical features of the electrochemical immunoassay were evaluated using the standard solution of S and N protein in buffer solution and untreated saliva with a detection limit equal to 19 ng/mL and 8 ng/mL in untreated saliva, respectively for S and N protein. Its effectiveness was assessed using cultured virus in biosafety level 3 and in saliva clinical samples comparing the data using the nasopharyngeal swab specimens tested with Real-Time PCR. The agreement of the data, the low detection limit achieved, the rapid analysis (30 min), the miniaturization, and portability of the instrument combined with the easiness to use and no-invasive sampling, confer to this analytical tool high potentiality for market entry as the first highly sensitive electrochemical immunoassay for SARS-CoV-2 detection in untreated saliva.

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Section: Introductionmentioning

confidence: 99%

Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

Fabiani

Saroglia

Galatà³

et al. 2021

Biosensors and Bioelectronics

353

316

View full text Add to dashboard Cite

show abstract

“…Currently, screen-printed electrodes (SPEs) seem to be the preferred option to develop fast and cost-effective in vitro diagnosis methods with a large variety of applications [ 69 ], due to the fact they can be mass-produced from a variety of materials and in a customable way (different geometries, multiplexed formats, nanoparticulated and with suitable tailor-made functionalities) [ 70 ]. In addition, their small dimensions allow the use of small sample volumes and their flat shape facilitates the incorporation of magnetic bioconjugates on their surface in a stable and reproducible way by simple magnetic attraction.…”

Section: Bioelectroanalytical Methods For the Determination Of Infmentioning

confidence: 99%

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

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“…The simplest procedure to modify SPEs is by deposition of the modifying agent onto the WE; this procedure is facilitated because of the planar nature of the SPE, so it can be performed through an automatic dispenser in a mass-producible way. However, the WE of an SPE can also be modified by adding the modifier to the ink before printing, by chemical adsorption or by electrochemical deposition (a good example is the in-situ generation of metallic nanoparticles) [ 32 , 33 , 34 ].…”

Section: Screen-printed Electrodes As Transducersmentioning

confidence: 99%

Screen-Printed Electrode-Based Sensors for Food Spoilage Control: Bacteria and Biogenic Amines Detection

et al. 2020

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Food spoilage is caused by the development of microorganisms, biogenic amines, and other harmful substances, which, when consumed, can lead to different health problems. Foodborne diseases can be avoided by assessing the safety and freshness of food along the production and supply chains. The routine methods for food analysis usually involve long analysis times and complex instrumentation and are performed in centralized laboratories. In this context, sensors based on screen-printed electrodes (SPEs) have gained increasing importance because of their advantageous characteristics, such as ease of use and portability, which allow fast analysis in point-of-need scenarios. This review provides a comprehensive overview of SPE-based sensors for the evaluation of food safety and freshness, focusing on the determination of bacteria and biogenic amines. After discussing the characteristics of SPEs as transducers, the main bacteria, and biogenic amines responsible for important and common foodborne diseases are described. Then, SPE-based sensors for the analysis of these bacteria and biogenic amines in food samples are discussed, comparing several parameters, such as limit of detection, analysis time, and sample type.

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Screen-Printed Electrodes Modified with Metal Nanoparticles for Small Molecule Sensing

Cited by 107 publications

References 98 publications

Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Screen-Printed Electrode-Based Sensors for Food Spoilage Control: Bacteria and Biogenic Amines Detection

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