Screen-printed carbon electrode/natural silica-ceria nanocomposite for electrochemical aptasensor application

Abstract

A nanocomposite of natural silica and ceria was synthesized to modify a screen-printed carbon electrode (SPCE) to develop an aptasensor to detect epithelial sodium channel (ENaC) protein in urine as a biomarker of hypertension. The method steps were the synthesis of natural silica-ceria nanocomposite using the hydrothermal method, obtaining of natural silica nanoparticles from the extraction of alkaline silica sand and ceria nanoparticles from cerium nitrate, modification of SPCE/natural silica-ceria, immobilization of aptamer through streptavidin-biotin, and detection of ENaC protein conc­entration. Box-Behnken’s design was employed to determine the optimal con­ditions of aptamer concentration (0.5 μg mL-1), streptavidin incubation time (30 min), and aptamer incubation time (1 hour), respectively. Differential pulse voltam­metry (DPV) characterization of the developed electrochemical aptasensor revealed that the [Fe(CN)6]3-/4- redox peak current increased from 3.190 to 9.073 μA, with detection and quantification limits of 0.113 and 0.343 ng mL-1, respectively. The method is proven as a simple and rapid method to monitor ENaC levels in urine samples.

Green Synthesis of Ceria Nanoparticles from Cassava Tubers for Electrochemical Aptasensor Detection of SARS-CoV-2 on a Screen-Printed Carbon Electrode

“…Nanoceria or ceria nanoparticle-based materials are widely used in many fields including catalyst, fuel cell, antioxidant, sensor, and biosensor applications. − In particular, nanoceria has been reported to improve sensitivity, selectivity, stability and maintain good long-term bioactivity in electrochemical biosensor techniques. ,, The modified nanoceria electrode can also drive biomolecules such as STV on the electrode surface. STV then is utilized to bind biotinylated bioreceptors such as antibodies, DNA, RNA, or aptamers to recognize the target biomolecules specifically. , This feature was utilized to modify the bioreceptor on the electrode surface in electrochemical biosensor applications, including the detection of glucose, diclofenac, and ENaC protein …”

“…30−32 In particular, nanoceria has been reported to improve sensitivity, selectivity, stability and maintain good long-term bioactivity in electrochemical biosensor techniques. 12,33,34 The modified nanoceria electrode can also drive biomolecules such as STV on the electrode surface. STV then is utilized to bind biotinylated bioreceptors such as antibodies, DNA, RNA, or aptamers to recognize the target biomolecules specifically.…”

CeO2@NH2 functionalized electrodes for the rapid detection of SARS-CoV-2 spike receptor binding domain

“…The published aptamers by Song et al have drawn the interest of researchers working on constructing electrochemical aptasensor. [27][28][29][30][31] An electrochemical aptamer-based sensor created by Idili et al was tested directly on saliva samples. Additionally, Zakashansky et al developed an aptasensor for saliva samples with a detection limit of 1 ng mL −1 .…”