Screen-printed carbon electrode-based electrochemical immunosensor for rapid detection of microalbuminuria

Tsai, Jang-Zern; Chen, Ching Jung; Settu, Kalpana; Lin, Yu; Chen, Chien Lung; Liu, Jen Tsai

doi:10.1016/j.bios.2015.11.002

Cited by 59 publications

(24 citation statements)

References 33 publications

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“…Unfortunately, these immunosensors are not commercially available. 13 Several limitations should be noted. First, the CU microalbumin was designed to detect microalbuminuria qualitatively for cost reduction in mass screening.…”

Section: Discussionmentioning

confidence: 99%

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Development and validation of point‐of‐care testing of albuminuria for early screening of chronic kidney disease

Vutthikraivit

Kiatamornrak

Boonkrai

et al. 2021

Clinical Laboratory Analysis

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Introduction: Chronic kidney disease (CKD) is a significant global health issue. As the prevalence of renal replacement therapy (RRT) in Thailand is increasing, early detection and management of CKD is the most important step to prevent CKD progression and the need for RRT. Current diagnostic tests for CKD are non-specific and expensive. We aimed to develop and validate antibody-based-albumin point-of-care testing (POCT) to detect patients with impaired kidney function at early stage. Methods: The prototype strip test was developed under the concept of competitive lateral flow immunochromatography assay, or strip test. Monoclonal antibodies (MAbs) to human serum albumin (HSA) were harvested from the hybridomas of spleen cells from immunized mice and mouse myeloma cells. Presence of MAbs was detected by enzyme-linked immunosorbent assay (ELISA). Spot urine was obtained from patients with kidney disease, type I, or type II Diabetes Mellitus upon their visit at King Chulalongkorn Memorial Hospital during 2018-2019. All samples were analyzedThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Discussionmentioning

confidence: 99%

“…The sensor could detect HSA in urine in a wide linear range from 10 to 300 ug/ml. Unfortunately, these immunosensors are not commercially available 13 …”

Section: Discussionmentioning

confidence: 99%

Development and validation of point‐of‐care testing of albuminuria for early screening of chronic kidney disease

Vutthikraivit

Kiatamornrak

Boonkrai

et al. 2021

Clinical Laboratory Analysis

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“…19), which shows that it has the highest sensitivity because of being strongest catalytic amplification, so it was selected to measure mAlb. Compared with the reported mAlb analysis method (Table S3), [31][32][33][34][35][37][38][39] this method is highly sensitive and easy to operate, and it is one of the best methods for detecting mAlb.…”

Section: Working Curvementioning

confidence: 99%

A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

Wen

Liang

et al. 2018

IJN

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BackgroundMicroalbuminuria (mAlb) detection is essential for the diagnosis and prognosis of nephrotic patients and hypoproteinemia. In this article, we develop a new surface-enhanced Raman scattering (SERS) quantitative analysis method to detect mAlb in urine.MethodsCombined the mAlb immunoreaction with gold nanoreaction of graphene oxide nanoribbons (GONR)-HAuCl4-H2O2, and used Victoria blue B (VBB) as molecular probe with a SERS peak at 1,615 cm−1, a new SERS strategy for quantitative analysis of trace mAlb in urine was established.ResultsThe linear range of SERS quantitative analysis method is from 0.065 to 2.62 ng/mL, with a detection limit of 0.02 ng/mL. The SERS method was applied to analysis of mAlb in urine with good accuracy and reliability, the relative standard deviation is 0.49%–2.28% and the recovery is 96.9%–109.8%.ConclusionThis study demonstrated that the new SERS quantitative analysis method is of high sensitivity, good selectivity and simplicity. It has been applied to analysis of mAlb in urine, with satisfactory results.

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“…To date, immunoassay is one of the most widely accepted methods for detection of trace cancer biomarkers due to that antibody has high affinity towards the corresponding antigen (Zhang et al 2008); (Mazzu-Nascimento et al 2017). Various immunoassay technologies have been developed based on antibody-antigen interaction, including but not limited to radioimmunoassay (RIA) (Bast Jr et al 1983), chemiluminescence immunoassay (CLIA) (Ge et al 2012;Zong et al 2012), fluorescence immunoassay (FIA) (Wacker and Niemeyer 2004), electrochemiluminescence (ECL) (Babamiri et al 2018), enzyme-linked immunosorbent assay (ELISA) (Gordon and Cross 1990) and electrochemical immunoassay (EIA) (Lai et al 2009;Tsai et al 2016). Of all these above-mentioned methods, the label-free electrochemical method, for its outstanding advantages of high selectivity and sensitivity, has been recognized as a powerful tool for multiplexed detection of cancer biomarkers (Sun and Ma 2013;Wei et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

Wang

Luo

Liu

et al. 2019

Biosensors and Bioelectronics

206

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Screen-printed carbon electrode-based electrochemical immunosensor for rapid detection of microalbuminuria

Cited by 59 publications

References 33 publications

Development and validation of point‐of‐care testing of albuminuria for early screening of chronic kidney disease

Development and validation of point‐of‐care testing of albuminuria for early screening of chronic kidney disease

A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

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