Screen of small fragment mutations within the sheep thyroid stimulating hormone receptor gene associated with litter size

Li, Ming; Zhou, Qian; Pan, Yejun; Lan, Xianyong; Zhang, Qingfeng; Pan, Chuanying; Mao, Chenchen

doi:10.1080/10495398.2021.1992415

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…Furthermore, 29 bp nucleotide sequence variation within the TSHR was significantly associated with litter size in sheep. In our experiments, the TSHR was significantly up-regulated in SP-LP, suggesting a role in the photoperiod, in agreement with previous findings ( 50 ). It is noteworthy that PPP3CC was different and significantly up-regulated in three periods, the expression of PPP3CC in the testis is critical for sperm viability and male fertility ( 51 ).…”

Section: Discussionsupporting

confidence: 94%

Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Wang

et al. 2022

Front. Endocrinol.

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Circular RNAs (circRNAs) are non-coding RNAs newly identified and play important roles in RNA regulation. However, little is known regarding photoperiods induced circRNAs in the thyroid gland. In this study, we performed a comprehensive analysis of circRNA profiles in the thyroid gland of OVX+E2 ewes at different photoperiods by whole transcriptome sequencing. A total of 37,470 novel circRNAs were detected in different photoperiods (42 days of short photoperiod treatment, SP42; 42 days of long photoperiod treatment, LP42; SP42 transfer to LP42, SPLP42), with a total of 817 circRNAs for SP42-LP42 (down: 132; up: 114), LP42-SPLP42 (down: 136; up: 112) and SP42-SPLP42 (down: 182; up: 141) having differentially expressed. Functional enrichment annotation analysis of DE-circRNAs for GO and KEGG by R package, features that influence photoperiod response in Sunite ewes through the Inositol phosphate metabolism, cGMP-PKG signaling pathway, Calcium signaling pathway, MAPK signaling pathway, and Oocyte meiosis. In addition, competitive endogenous RNA (ceRNA) network analysis revealed target binding sites for identified miRNAs in DE-cirRNAs such as oar-miR-10b, oar-miR-200c, oar-miR-21, oar-miR-370-3p, oar-miR-377-3p, oar-miR-181a, oar-miR-432, and oar-miR-495-3p. These results of this study will provide some new information for understanding circRNA function as well as the changes in the sheep thyroid gland under different photoperiods.

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Section: Discussionsupporting

confidence: 94%

Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Wang

et al. 2022

Front. Endocrinol.

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“…TSHB is an important component of thyroid stimulating hormone (TSH), deciding the biological specificity and synthesis rate of TSH, and is closely related to the photoperiod of seasonal reproduction in mammals (Huang et al, 2013). TSHR is a thyroid-stimulating hormone receptor that plays an important role in seasonal estrus and reproduction in goats (Yurchenko et al, 2019;Li et al, 2021a). PTGS2, ESR2, and SERPINB11 are relevant to estrogen and follicular growth (Xu et al, 2018).…”

Section: Candidate Genes Associated With Reproduction Traitsmentioning

confidence: 99%

Whole-genome resequencing reveals genetic diversity and selection characteristics of dairy goat

Xiong

Bao

et al. 2023

Front. Genet.

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The dairy goat is one of the earliest dairy livestock species, which plays an important role in the economic development, especially for developing countries. With the development of agricultural civilization, dairy goats have been widely distributed across the world. However, few studies have been conducted on the specific characteristics of dairy goat. In this study, we collected the whole-genome data of 89 goat individuals by sequencing 48 goats and employing 41 publicly available goats, including five dairy goat breeds (Saanen, Nubian, Alpine, Toggenburg, and Guanzhong dairy goat; n = 24, 15, 11, 6, 6), and three goat breeds (Guishan goat, Longlin goat, Yunshang Black goat; n = 6, 15, 6). Through compared the genomes of dairy goat and non-dairy goat to analyze genetic diversity and selection characteristics of dairy goat. The results show that the eight goats could be divided into three subgroups of European, African, and Chinese indigenous goat populations, and we also found that Australian Nubian, Toggenburg, and Australian Alpine had the highest linkage disequilibrium, the lowest level of nucleotide diversity, and a higher inbreeding coefficient, indicating that they were strongly artificially selected. In addition, we identified several candidate genes related to the specificity of dairy goat, particularly genes associated with milk production traits (GHR, DGAT2, ELF5, GLYCAM1, ACSBG2, ACSS2), reproduction traits (TSHR, TSHB, PTGS2, ESR2), immunity traits (JAK1, POU2F2, LRRC66). Our results provide not only insights into the evolutionary history and breed characteristics of dairy goat, but also valuable information for the implementation and improvement of dairy goat cross breeding program.

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“…2 A, Table S2 ). As previously reported, one of the functions of TSHR was to regulate the development of the thyroid gland and the secretion of thyroid hormone, which in turn plays an important role in the seasonal reproduction of mammals [ 48 , 49 ]. TSHR could catalyze cAMP synthesis, as a secondary messenger, cAMP was a major contributor to the process of releasing follicle-stimulating hormone and luteinizing hormone.…”

Section: Discussionmentioning

confidence: 99%

Comparative whole-genome resequencing to uncover selection signatures linked to litter size in Hu Sheep and five other breeds

Zhong,

Hou,

Zhao

et al. 2024

BMC Genomics

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Hu sheep (HS), a breed of sheep carrying the FecB mutation gene, is known for its “year-round estrus and multiple births” and is an ideal model for studying the high fecundity mechanisms of livestock. Through analyzing and comparing the genomic selection features of Hu sheep and other sheep breeds, we identified a series of candidate genes that may play a role in Hu sheep’s high fecundity mechanisms. In this study, we conducted whole-genome resequencing on six breeds and screened key mutations significantly correlated with high reproductive traits in sheep. Notably, the CC2D1B gene was selected by the fixation index (FST) and the cross-population composite likelihood ratio (XP-CLR) methods in HS and other five breeds. It was worth noting that the CC2D1B gene in HS was different from that in other sheep breeds, and seven missense mutations have been identified. Furthermore, the linkage disequilibrium (LD) analysis revealed a strong linkage disequilibrium in this specific gene region. Subsequently, by performing different grouping based on FecB genotypes in Hu sheep, genome-wide selective signal analysis screened several genes related to reproduction, such as BMPR1B and PPM1K. Besides, FST analysis identified functional genes related to reproductive traits, including RHEB, HSPA2, PPP1CC, HVCN1, and CCDC63. Additionally, a missense mutation was found in the CCDC63 gene and the haplotype was different between the high reproduction (HR) group and low reproduction (LR) group in HS. In summary, we discovered genetic differentiation among six distinct breeding sheep breeds at the whole genome level. Additionally, we identified a set of genes which were associated with reproductive performance in Hu sheep and visualized how these genes differed in different breeds. These findings laid a theoretical foundation for understanding genetic mechanisms behind high prolific traits in sheep.

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Screen of small fragment mutations within the sheep thyroid stimulating hormone receptor gene associated with litter size

Cited by 5 publications

References 32 publications

Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Whole-genome resequencing reveals genetic diversity and selection characteristics of dairy goat

Comparative whole-genome resequencing to uncover selection signatures linked to litter size in Hu Sheep and five other breeds

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