Screen anticancer drug in vitro using resonance light scattering technique

Chen, Zhanguang; Liu, Guoliang; Chen, Meizhen; Xu, Benjie; Peng, Yue; Chen, Maohuai; Wu, Mingyao

doi:10.1016/j.talanta.2008.09.016

Cited by 32 publications

(12 citation statements)

References 32 publications

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“…The advantages of the RLS method include its simplicity, sensitivity and quickness, and it is thus widely applied to investigate proteins . The investigation of proteins using the RLS technique is mainly dependent upon enhancement of the RLS intensity, which is associated with the formation of complexes between RLS probes and proteins . Moreover, RLS is an important technique for administering molecular assemblies and for describing the extended aggregates of chromophores .…”

Section: Resultsmentioning

confidence: 99%

Comparison of the binding behavior of FCCP with HSA and HTF as determined by spectroscopic and molecular modeling techniques

et al. 2013

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The interaction of carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP) with human serum albumin (HSA) and human transferrin (HTF) was investigated using multiple spectroscopy, molecular modeling, zeta-potential and conductometry measurements of aqueous solutions at pH 7.4. The fluorescence, UV/vis and polarization fluorescence spectroscopy data disclosed that the drug-protein complex formation occurred through a remarkable static quenching. Based on the fluorescence quenching, two sets of binding sites with distinct affinities for FCCP existed in the two proteins. Steady-state and polarization fluorescence analysis showed that there were more affinities between FCCP and HSA than HTF. Far UV-CD and synchronous fluorescence studies indicated that FCCP induced more structural changes on HSA. The resonance light scattering (RLS) and zeta-potential measurements suggested that HTF had a greater resistance to drug aggregation, whereas conductometry measurements expressed the presence of free ions improving the resistance of HSA to aggregation. Thermodynamic measurements implied that a combination of electrostatic and hydrophobic forces was involved in the interaction between FCCP with both proteins. The phase diagram plots indicated that the presence of second binding site on HSA and HTF was due to the existence of intermediate structures. Site marker competitive experiments demonstrated that FCCP had two distinct binding sites in HSA which were located in sub-domains IIA and IIIA and one binding site in the C-lobe of HTF as confirmed by molecular modeling. The obtained results suggested that both proteins could act as drug carriers, but that the HSA potentially had a higher capacity for delivering FCCP to cancerous tissues.

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Section: Resultsmentioning

confidence: 99%

Comparison of the binding behavior of FCCP with HSA and HTF as determined by spectroscopic and molecular modeling techniques

et al. 2013

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“…The inhibition rate (IR) was calculated as the formula: IR = (OD control group − OD experimental group )/OD control group × 100%262728. IR ≥ 50% was defined as highly sensitive; IR 30% ~ 50% as moderately sensitive; and IR ≤ 30% as resistant.…”

Section: Methodsmentioning

confidence: 99%

ABCC3 as a marker for multidrug resistance in non-small cell lung cancer

Zhao

et al. 2013

Sci Rep

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Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 was one of the most up-regulated genes in the resistant group. ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS. ABCC3 expression may serve as a marker for MDR and predictor for poor clinical outcome of NSCLC.

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“…However, Rayleigh scattering will deviate from the law and the intensity of some wavelengths will rapidly increase if the wavelength of the incident beam is close to that of the absorption band of the aggregated molecular particles. Recently, RLS has become a useful technique for the determination of traces of inorganic ions [10,11], nucleic acids [12,13], surfactants [14,15], drugs [16][17][18] and proteins [19,20], as well as for the studies of interactions of biopolymers with their probes [21].…”

Section: Introductionmentioning

confidence: 99%

Determination of lead at nanogram level in water samples by resonance light scattering technique using tetrabutyl ammonium bromide as a molecular probe

Cui¹,

Yun²,

Hui³

et al. 2012

Bull. Chem. Soc. Eth.

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ABSTRACT. A novel method of chemistry applicable to the determination of trace lead in water samples based on the resonance light scattering (RLS) technique has been developed. In dilute phosphoric acid medium, in the presence of a large excess of I -, Pb(II) can form [PbI4] 2-, which further reacts with tetrabutyl ammonium bromide (TBAB) to form an ion-association compound. This results in significant enhancement of RLS intensity and the appearance of the corresponding RLS spectral characteristics. The maximum scattering peak of the system exists at 402 nm. Under optimum conditions, there is a linear relationship between the relative intensity of RLS and concentration of Pb(II) in the range of 0.04-1.8 µg/mL for the system with a low detection limit of 0.74 ng/mL for Pb(II). Based on this fact, a simple, rapid, and sensitive method has been developed for the determination of Pb(II) at nanogram level by RLS technique using a common spectrofluorimeter. This analytical system was successfully applied to determining trace amounts of Pb(II) in water samples that agree well with the results by atomic absorbance spectrometry (AAS).

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Screen anticancer drug in vitro using resonance light scattering technique

Cited by 32 publications

References 32 publications

Comparison of the binding behavior of FCCP with HSA and HTF as determined by spectroscopic and molecular modeling techniques

Comparison of the binding behavior of FCCP with HSA and HTF as determined by spectroscopic and molecular modeling techniques

ABCC3 as a marker for multidrug resistance in non-small cell lung cancer

Determination of lead at nanogram level in water samples by resonance light scattering technique using tetrabutyl ammonium bromide as a molecular probe

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