Scratching the niche that controls Caenorhabditis elegans germline stem cells

Byrd, Dana T.; Kimble, Judith

doi:10.1016/j.semcdb.2009.09.005

Cited by 71 publications

(69 citation statements)

References 80 publications

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“…For example, in C. elegans a group of ~220 mitotic germ cells are maintained throughout life at the distal end of each gonad ('mitotic zone', Fig. 1A) (Byrd and Kimble, 2009;Hubbard, 2007). The progeny of these cells differentiate in a distal-to-proximal gradient along the length of the gonad.…”

Section: Introductionmentioning

confidence: 99%

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The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells

Merritt

Seydoux

2010

Development

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SUMMARYFBF-1 and FBF-2 (collectively FBF) are two nearly identical Puf-domain RNA-binding proteins that regulate the switch from mitosis to meiosis in the C. elegans germline. In germline stem cells, FBF prevents premature meiotic entry by inhibiting the expression of meiotic regulators, such as the RNA-binding protein GLD-1. Here, we demonstrate that FBF also directly inhibits the expression of structural components of meiotic chromosomes. HIM-3, HTP-1, HTP-2, SYP-2 and SYP-3 are components of the synaptonemal complex (SC) that forms between homologous chromosomes during meiotic prophase. In wild-type germlines, the five SC proteins are expressed shortly before meiotic entry. This pattern depends on FBF binding sites in the 3Ј UTRs of the SC mRNAs. In the absence of FBF or the FBF binding sites, SC proteins are expressed precociously in germline stem cells and their precursors. SC proteins aggregate and SC formation fails at meiotic entry. Precocious SC protein expression is observed even when meiotic entry is delayed in fbf mutants by reducing GLD-1. We propose that parallel regulation by FBF ensures that in wild-type gonads, meiotic entry is coordinated with just-in-time synthesis of synaptonemal proteins.

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Section: Introductionmentioning

confidence: 99%

“…Meiotic entry is regulated by a complex network of RNA-binding proteins (Byrd and Kimble, 2009). Central to the network are FBF-1 and FBF-2, two highly similar Puf-domain RNA-binding proteins known collectively as FBF (Crittenden et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells

Merritt

Seydoux

2010

Development

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“…For example, bone morphogenetic protein (BMP) in the Drosophila female GSC niche is necessary and sufficient for GSC self-renewal (Xie, 2012). This is also true for Notch in the C. elegans GSC niche (Byrd and Kimble, 2009;Kimble and Crittenden, 2007). However, for most stem cell types, the simultaneous activation of several pathways is needed for continuous stem cell self-renewal.…”

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confidence: 99%

Adhesion in the stem cell niche: biological roles and regulation

2013

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Stem cell self-renewal is tightly controlled by the concerted action of stem cell-intrinsic factors and signals within the niche. Niche signals often function within a short range, allowing cells in the niche to self-renew while their daughters outside the niche differentiate. Thus, in order for stem cells to continuously self-renew, they are often anchored in the niche via adhesion molecules. In addition to niche anchoring, however, recent studies have revealed other important roles for adhesion molecules in the regulation of stem cell function, and it is clear that stem cell-niche adhesion is crucial for stem cell self-renewal and is dynamically regulated. Here, we highlight recent progress in understanding adhesion between stem cells and their niche and how this adhesion is regulated.

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“…The lag-2 is one of C. elegans Notch ligands and expressed in the DTC (Henderson et al, 1994;. Normally, wild-type hermaphrodites have two DTCs (Byrd and Kimble, 2009) (Fig. 2D).…”

Section: Elegans Canonical Wnt/β-catenin Signalingmentioning

confidence: 99%