Scrapie e seu diagnóstico diferencial em ovinos no Mato Grosso do Sul

Martins, H; Carvalho, Nilton M.; Ribas, Nickolly L.K.S.; Driemeier, David; Lemos, Ricardo A.A.; Guimarães, Euripedes Batista

doi:10.1590/s0100-736x2012001200004

Cited by 2 publications

(3 citation statements)

References 48 publications

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“…As described in other outbreaks in Brazil and the world, the disease occurred without an apparent macroscopic lesion. Loss of wool and weight can be related but are not characteristic of the disease (MARTINS et al, 2012;ESTEVES et al, 2021). Histological lesions in classic scrapie are at the brain stem (obex), characteristic of vacuolization of neurons and neuropil without inflammatory reaction, as described in this study.…”

Section: Discussionsupporting

confidence: 48%

“…Importantly, only one animal in this outbreak, had characteristic lesions in the obex. This intensity is uncommon, as usually the animals have very characteristic and evident neuronal or neuropil vacuolization in the obex in outbreaks, as observed in some studies in Brazil (MARTINS et al, 2012;ANDRADE et al, 2015). Fourteen sheep were positive by the IHC in the lymphoid tissues, however, only two animals that were positive for IHC in the obex.…”

Section: Discussionmentioning

confidence: 80%

“…Histological lesions in classic scrapie are at the brain stem (obex), characteristic of vacuolization of neurons and neuropil without inflammatory reaction, as described in this study. However, these lesions have also been described to a lesser extent in the midbrain, pons, medulla oblongata, and lateral and ventral horns of the spinal cord (MARTINS et al, 2012). There is no established pattern in the distribution of atypical scrapie lesions, but lesions in the cortex and cerebellum without changes in the obex have been described (GREENLEE, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Use of immunohistochemistry and prion protein gene genotyping for detection of Scrapie in sheep in the state of Santa Catarina, Brazil

Quevedo

Andrade

Hemckmeier³

et al. 2024

Cienc. Rural

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Scrapie is a contagious disease of sheep and goats caused by prions (PrPSc). This study described an outbreak of Scrapie in sheep in the state of Santa Catarina, Brazil. An 1-year and 3-month-old sheep developed clinical signs characterized by motor incoordination of the pelvic limbs, pruritus and alopecia for three days. The 38 sheep from the flock that were over 1 year of age underwent biopsies of the third eyelid and rectal mucosa, in addition to anti-PrPsc immunohistochemistry (IHC). Blood containing EDTA was collected for PRNP gene genotyping from these sheep. Of the 38, 16 (42.10%) had immunostaining againstPrPSc. IHC-positive animals were euthanized and necropsied, as well as lambs from positive mothers. Different organs of the 19 necropsied animals were collected in 10% buffered formalin for histopathological examination and anti-PrPSc IHC of the obex. The histopathology of the obex of the female with neurological signs presented discrete multifocal vacuolization of the cytoplasm of neurons and neuropil. The anti-PrPSc IHC showed that two out of the 19 obex samples had cytoplasmic immunostaining in neurons. The genotypes reported were ARQ/ARQ in 47.36%, ARR/ARQ in 36.84%, ARQ/VRQ in 10.52% and ARQ/VRR in 5.28%. The genotyping helps to identify susceptible animals and select animals more resistant to the development of Scrapie. The anti-PrPSc IHC from lymphoid biopsies, and genotyping demonstrated the high number of positive sheep classified in susceptible group.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Use of immunohistochemistry and prion protein gene genotyping for detection of Scrapie in sheep in the state of Santa Catarina, Brazil

Quevedo

Andrade

Hemckmeier³

et al. 2024

Cienc. Rural

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Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie

Gonçalves

Soares

Sanches

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.Index terms: genotyping, PRNP gene, single nucleotide polymorphisms, transmissible spongiform encephalopathies. Perfil genotípico de ovinos Crioulos pantaneiros quanto à suscetibilidade ou resistência à scrapieResumo -O objetivo deste trabalho foi avaliar o perfil genotípico específico para scrapie, nos códons 136, 154 e 171, no gene PRNP de ovinos Crioulos pantaneiros. O DNA genômico foi extraído de amostras de sangue de 66 ovinos, e as regiões de interesse da fita de DNA foram amplificadas por PCR. Foram identificados

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Scrapie e seu diagnóstico diferencial em ovinos no Mato Grosso do Sul

Cited by 2 publications

References 48 publications

Use of immunohistochemistry and prion protein gene genotyping for detection of Scrapie in sheep in the state of Santa Catarina, Brazil

Use of immunohistochemistry and prion protein gene genotyping for detection of Scrapie in sheep in the state of Santa Catarina, Brazil

Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie

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