1987
DOI: 10.1016/0014-4827(87)90014-0
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Scrape-loading and dye transfer

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Cited by 625 publications
(135 citation statements)
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“…It does not diffuse through intact plasma membranes, and its low molecular weight (M r 457.2) permits its transmission from one cell to another presumably through patent gap junctions. Rhodamine dextran is a high molecular weight polymer (M r 10,000), and it cannot diffuse through intact plasma membranes nor cross the gap junctions; therefore, it is used as a control to differentiate dye transfer from diffusion because of cell damage (30). Cells were then fixed with 2.5% (w/v) paraformaldehyde, and the numbers of cells transferring dye are counted using a standard fluorescent or laser-scanning confocal microscope.…”
Section: Methodsmentioning
confidence: 99%
“…It does not diffuse through intact plasma membranes, and its low molecular weight (M r 457.2) permits its transmission from one cell to another presumably through patent gap junctions. Rhodamine dextran is a high molecular weight polymer (M r 10,000), and it cannot diffuse through intact plasma membranes nor cross the gap junctions; therefore, it is used as a control to differentiate dye transfer from diffusion because of cell damage (30). Cells were then fixed with 2.5% (w/v) paraformaldehyde, and the numbers of cells transferring dye are counted using a standard fluorescent or laser-scanning confocal microscope.…”
Section: Methodsmentioning
confidence: 99%
“…The scrape-loading dye transfer assay was performed based on the modified procedure described by El-Fouly et al (49). In this method, cells were scratched in the presence of two types of fluorescence dyes: Lucifer Yellow (LY) (457 Da), which can penetrate through gap junction channels, and rhodamine dextran (RD) (10 kDa), which is too large to pass through the channels, thus serving as a tracer dye for the cells originally receiving the dye.…”
Section: Sds-page and Westernmentioning
confidence: 99%
“…GJIC was assessed in WB cells by scrape-loading/dye transfer (SL/DT) [11] [12]. Cells were cultured to confluence in six well dishes then fluorescent Lucifer Yellow CH was added from stock solution (5% w/v in PBS) to a final concentration of 0.05%.…”
Section: Assessment Of Gjicmentioning
confidence: 99%