α‐Mangostin induces oxidative damage, mitochondrial dysfunction, and apoptosis in a triple‐negative breast cancer model

Abstract

Triple-negative breast cancer (TNBC) does not express estrogen receptor, progesterone receptor, and human epidermal growth factor receptor; therefore, TNBC lacks targeted therapy, and chemotherapy is the only available treatment for this illness but causes side effects. A putative strategy for the treatment of TNBC could be the use of the polyphenols such as α-Mangostin (α-M), which has shown anticancerogenic effects in different cancer models and can modulate the inflammatory and prooxidant state in several pathological models. The redox state, oxidative stress (OS), and oxidative damage are highly related to cancer development and its treatment. Thus, this study aimed to evaluate the effects of α-M on redox state, mitochondrial metabolism, and apoptosis in 4T1 mammary carcinoma cells. We found that α-M decreases both protein levels and enzymatic activity of catalase, and increases reactive oxygen species, oxidized proteins and glutathione disulfide, which demonstrates that α-M induces oxidative damage. We also found that α-M promotes mitochondrial dysfunction by abating basal respiration, the respiration ligated to oxidative phosphorylation (OXPHOS), and the rate control of whole 4T1 cells. Additionally, α-M also decreases the levels of OXPHOS subunits of mitochondrial complexes I, II, III, and adenosine triphosphate synthase, the activity of mitochondrial complex I as well as the levels of peroxisome proliferator-activated receptor-gamma co-activator 1α, showing a mitochondrial mass reduction. Then, oxidative damage and mitochondrial dysfunction induced by α-M induce apoptosis of 4T1 cells, which is evidenced by B cell lymphoma 2 decrease and caspase 3 cleavage. Taken together, our results suggest that α-M induces OS and mitochondrial dysfunction, resulting in 4T1 cell death through apoptotic mechanisms.

Stimulation of Hemolysis and Eryptosis by α-Mangostin through Rac1 GTPase and Oxidative Injury in Human Red Blood Cells

“…Although α-MG is reported to exert antioxidant and anti-inflammatory effects [ 36 , 37 ], our results demonstrate that it can also promote ROS accumulation ( Figure 6 A–C) as previously seen in other cell types [ 8 , 38 ]. Oxidative stress primes RBCs for eryptosis, especially given the vulnerability of these cells to oxygen damage.…”

Different Modes of Mechanism of Gamma-Mangostin and Alpha-Mangostin to Inhibit Cell Migration of Triple-Negative Breast Cancer Cells Concerning CXCR4 Downregulation and ROS Generation

“…In the present study, γ-mangostin and α-mangostin increased ROS levels, and NAC addition nearly eliminated the ROS levels induced by these compounds. Other studies have reported a similar effect of α-mangostin on inducing cellular ROS production ( 41 , 42 ). Alpha-mangostin inhibited catalase activity in TNBC 4T1 cells but not antioxidant activity, resulting in the pro-oxidant effect on cancer cells ( 42 ).…”

“…In the present study, γ-mangostin and α-mangostin increased ROS levels, and NAC addition nearly eliminated the ROS levels induced by these compounds. Other studies have reported a similar effect of α-mangostin on inducing cellular ROS production (41,42).…”

“…Alpha-mangostin inhibited catalase activity in TNBC 4T1 cells but not antioxidant activity, resulting in the pro-oxidant effect on cancer cells (42). Moreover, α-mangostin-induced ROS overproduction might activate the PI3K/AKT signaling pathway, inhibiting cancer cell proliferation, migration, and induction of mitochondrial dysfunction that mediates apoptosis in MDA-MB-231 cells (23).…”