<scp>VII</scp>. Yeast sequencing reports. Cloning and characterization of a sulphite‐resistance gene of <i>Saccharomyces cerevisiae</i>

Casalone, Enrico; Colella, C.; Daly, Simona; Fontana, Silvia; Torricelli, Ilaria; Polsinelli, Mario

doi:10.1002/yea.320100812

Cited by 24 publications

(22 citation statements)

References 28 publications

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“…Another gene involved in sulphite resistance in yeast is FZF1 (Casalone et al, 1992(Casalone et al, , 1994, a positive regulator of SSU1 transcription (Avram & Bakalinsky, 1996;Avram et al, 1999), which interacts with a DNA region immediately upstream from SSU1 (Avram et al, 1999). Sulphite resistance has been attributed, in particular, to a dominant FZF1 allele (FZF1-4) and to the overexpression of wildtype FZF1 (Avram & Bakalinsky, 1996;Avram et al, 1999;Casalone et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast

2010

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Sulphite is widely used as a preservative in foods and beverages for its antimicrobial and antioxidant activities, particularly in winemaking where SO 2 is frequently added. Thus, sulphite resistance mechanisms have been extensively studied in the fermenting yeast Saccharomyces cerevisiae. Sulphite detoxification, involving a plasma membrane protein encoded by the SSU1 gene, is the most efficient resistance mechanism in S. cerevisiae. In this study, we characterized the unusual expression pattern of SSU1 in the wine strain 71B. We provide, for the first time, evidence of SSU1 induction by sulphite. The study of SSU1 expression during fermentation and in different growth conditions showed that sulphite is the main regulator of SSU1 expression, explaining its specific pattern. Combining analyses of gene expression and growth behaviour in response to sulphite, we found that 71B displayed unique behavioural patterns in response to sulphite pre-adaptation that may be explained by changes in SSU1 expression. Examination of the genomic organization of the SSU1 locus and sequencing of the region revealed three different alleles in 71B, two of which corresponded to translocated VIII-XVI forms. The lack of differences between promoter regions suggests that this inducible SSU1 expression pattern is due to modification of regulatory/signalling pathways.

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Section: Introductionmentioning

confidence: 99%

“…Sulphite resistance has been attributed, in particular, to a dominant FZF1 allele (FZF1-4) and to the overexpression of wildtype FZF1 (Avram & Bakalinsky, 1996;Avram et al, 1999;Casalone et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast

2010

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“…The effective use of sulfite as a bacterial inhibitor in wine is due to the relative resistance of the wine yeast Saccharomyces cerevisiae to sulfite. Resistance of S. cerevisiae to either sulfite or RNS is mediated by transcriptional responses that require the Five Zinc Finger transcription factor FZF1 (Casalone et al 1994;Avram et al 1999;Park and Bakalinsky 2000;Sarver and DeRisi 2005;Engle and Fay 2012). Although the mechanism of sulfite resistance in S. cerevisiae has been investigated, responses to sulfite by other physiological relevant yeasts, such as the opportunistic fungal pathogen C. albicans, are less clearly understood (Nadai et al 2016).…”

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confidence: 99%

Adaptation of Candida albicans to Reactive Sulfur Species

Chebaro

Lorenz²,

et al. 2017

Genetics

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Candida albicans is an opportunistic fungal pathogen that is highly resistant to different oxidative stresses. How reactive sulfur species (RSS) such as sulfite regulate gene expression and the role of the transcription factor Zcf2 and the sulfite exporter Ssu1 in such responses are not known. Here, we show that C. albicans specifically adapts to sulfite stress and that Zcf2 is required for that response as well as induction of genes predicted to remove sulfite from cells and to increase the intracellular amount of a subset of nitrogen metabolites. Analysis of mutants in the sulfate assimilation pathway show that sulfite conversion to sulfide accounts for part of sulfite toxicity and that Zcf2-dependent expression of the SSU1 sulfite exporter is induced by both sulfite and sulfide. Mutations in the SSU1 promoter that selectively inhibit induction by the reactive nitrogen species (RNS) nitrite, a previously reported activator of SSU1, support a model for C. albicans in which Cta4-dependent RNS induction and Zcf2-dependent RSS induction are mediated by parallel pathways, different from S. cerevisiae in which the transcription factor Fzf1 mediates responses to both RNS and RSS. Lastly, we found that endogenous sulfite production leads to an increase in resistance to exogenously added sulfite. These results demonstrate that C. albicans has a unique response to sulfite that differs from the general oxidative stress response, and that adaptation to internal and external sulfite is largely mediated by one transcription factor and one effector gene.

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“…We also showed that FZF1 is a multicopy suppressor of the sulfite sensitivity of a grr1 mutant. FZF1 encodes a five-zinc-finger putative transcription factor (5), and a particular allele was independently found to confer dominant resistance to sulfite (6). Here we show that the product of the SSU1 gene, also implicated in sulfite sensitivity, is a plasma membrane protein, and we propose potential relationships among SSU1 and three other genes involved in protection against sulfite, FZF1, SSU3, and GRR1.…”

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confidence: 63%

SSU1 encodes a plasma membrane protein with a central role in a network of proteins conferring sulfite tolerance in Saccharomyces cerevisiae

Avram

Bakalinsky

1997

J Bacteriol

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The Saccharomyces cerevisiae SSU1 gene was isolated based on its ability to complement a mutation causing sensitivity to sulfite, a methionine intermediate. SSU1 encodes a deduced protein of 458 amino acids containing 9 or 10 membrane-spanning domains but has no significant similarity to other proteins in public databases. An Ssu1p-GFP fusion protein was localized to the plasma membrane. Multicopy suppression analysis, undertaken to explore relationships among genes previously implicated in sulfite metabolism, suggests a regulatory pathway in which SSU1 acts downstream of FZF1 and SSU3, which in turn act downstream of GRR1.

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VII. Yeast sequencing reports. Cloning and characterization of a sulphite‐resistance gene of Saccharomyces cerevisiae

Cited by 24 publications

References 28 publications

A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast

A sulphite-inducible form of the sulphite efflux gene SSU1 in a Saccharomyces cerevisiae wine yeast

Adaptation of Candida albicans to Reactive Sulfur Species

SSU1 encodes a plasma membrane protein with a central role in a network of proteins conferring sulfite tolerance in Saccharomyces cerevisiae

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