SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma

Abstract

Background: Lymph node metastasis (LNM) is an important cause leading to recurrence and development of head and neck carcinoma (HNC), with the precise mechanisms unclear. Thus, we aimed to identify the key genes involved in LNM and further evaluate their expressions and roles.Methods: A cohort of HNC in the TCGA was analyzed. The study involved three phases (one screening and two validation phases). First, the differentially expressed genes regarding LNM were screened, from which a key gene was identified by a series of data mining approaches. Then, the expressions and roles of the key gene were validated in HNC through bioinformatics. Afterward, the expression of the key gene was detected by qPCR, western blot, and Immunohistochemistry based on a cell model and a tissue microarray. Further, colony formation and transwell migration and invasion assays were used to evaluate the roles of the key gene.Results: SPP1 was overexpressed in HNC tissues and was identified as the key gene.Overexpression of SPP1 in HNC was correlated with advanced pathological stages and T-stage, as well as the presence of LNM, which predicted poor prognosis. The expression of SPP1 was closely associated with the infiltration of immune cells in HNC, especially M2 macrophages. Lab experiments confirmed that SPP1 silence in HNC cells resulted in weakened invasive and metastatic abilities. Conclusion:This study reveals that SPP1 may be a key gene associated with LNM in HNC, raising the possibility of SPP1 as a target for HNC prevention and treatment.

<i>SERPINE1</i> as an Independent Prognostic Marker and Therapeutic Target for Nicotine-Related Oral Carcinoma

“…The RNA interference technique was utilized. Stable SERPINE1 -knockdown cell lines were established according to the methods described recently [ 14 ]. Two cancer cell lines, CAL27 and SAS, were chosen for further exploration.…”

“…A tissue microarray of oral cancer patients (HOraC060PG01) was purchased from Shanghai Outdo Biotech Company. The characteristic of the patients was described A c c e p t e d A r t i c l e previously [14]. SERPINE1 protein expression was measured by immunohistochemistry (IHC) assay.…”

Risk prediction for Dermatomyositis-associated hepatocellular carcinoma

“…And in pancreatic adenocarcinoma, SPP1 expression levels were associated with patient prognosis and immune regulation (Chen et al, 2022), and in lung adenocarcinoma, SPP1 was found to be associated with drug resistance (Matsubara et al, 2022). In a recent study, researchers further investigated the level of serum SPP1 autoantibodies in patients with esophageal squamous cell carcinoma (ESCC) and showed that its expression was signi cantly upregulated compared to the normal group, suggesting that anti-SPP1 autoantibodies are a novel biomarker for the detection of ESCC , and SPP1 is important in prostate cancer (PCa), head and neck cancer (HNC), cervical squamous cell carcinoma (SCC) and other cancers (Zhang et al, 2022, Feng et al, 2022.…”

MYO1B as a prognostic biomarker and a therapeutic target in Arecoline‐associated oral carcinoma

“…Stable MYO1B-knockdown cell lines were established according to the methods described in our previous study. 38 DOK-trans and SAS were selected for further study. The cells with stable MYO1B-silence were named sh-MYO1B-DOK/trans and sh-MYO1B-SAS, while the relevant controls were named sh-NC-DOK/ trans and sh-NC-SAS, respectively.…”