SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities

Abstract

The flagellar beating of a spermatozoa's axoneme is caused by the varying activation and inactivation of dynein molecules. Dynein, axonemal, light chain 4 (DNAL4) is a functional candidate gene for sperm motility as it encodes a small subunit of the dyneins. We resequenced the porcine DNAL4 using three artificial insemination (AI) boars each with high (>68%) or low (<60%) motility, and detected 23 SNP. These were then genotyped for 82 AI boars. Using spermatological records, significantly negative genetic correlations between ejaculate volume (VOL) and the further spermatological parameters concentration (CONC) (r = -.43), motility of undiluted semen (MOTUD) (r = -.09), motility after 24 h (MOT1) (r = -.17) and after 48 hr (MOT2) (r = -.23) were estimated. Significantly positive correlations existed between CONC and MOT1 (r = .07) as well as MOT2 (r = .10), between MOTUD and MOT1 (r = .33), between MOTUD and MOT2 (r = .36), and finally between MOT1 and MOT2 (r = .70). Significantly negatively correlated were all motility traits with the parameters abnormal acrosome (AA) (MOTUD r = -.06; MOT1 r = -.08, and MOT2 r = -.1) and presence of cytoplasmic droplet (CD) (MOTUD r = -.07; MOT1 r = -.08; MOT2 r = -.07). Association analyses (single marker regression model; SMR) propose that SNP g.1007A>G, located in the second intron, reduces motility significantly (MOTUD -4.59%; MOT1 -10.33%; MOT2 -19.37%). According to the dominant-recessive model (DRM), genotype AA is always superior compared to genotypes AG and GG (i.e. MOTUD 67.67%, 64.16% and 53.91%; MOT1 54.17%, 43.75% and 28.44%; MOT2 44.12%, 24.91% and 4.97%). The average effect of gene substitution (g.1007A>G) on abnormal midpiece (AM) was 0.71%, the genotypic values-as expressed by LSmeans-were 0.1 (AA) and 0.81 (AG).

Sperm Characteristics in Microminipigs

“…The sperm of microminipigs had a weakly alkaline pH, similar to common pig breeds (33) and other strains of mini pigs (34). Quality characteristics such as sperm motility, morphological normality, and acrosome normality varied among individuals but were similar to those for other pig breeds (35)(36)(37)(38) and other mini pig strains (33). Ejaculate volume and total number of spermatozoa from microminipigs were smaller than those of standard pig breeds.…”

Identification and Functional Annotation of Genes Related to Bone Stability in Laying Hens Using Random Forests

“…The genotypic effect was analysed for those SNPs located in intragenic or in flanking genomic regions of candidate genes, which have previously been shown to be significantly associated with a bone trait (see Table 1 ). SNP effects for each locus were analysed as described by Wiedemann et al [ 47 ]. For this purpose, the actual SNP genotypes were coded as ‘0’ (AA), ‘1’ (AB), or ‘2’ (BB), with the B allele representing the minor allele.…”

Molekulargenetische Analysen fortpflanzungsrelevanter Fragestellungen beim landwirtschaftlichen Nutztier

“…Eine ausschließlich in den Spermien exprimierte Variante von GAPDH, GAPDS, scheint hauptverantwortlich für die Motilität der Spermien zu sein (Miki et al, 2004). Sollte nicht ausreichend Energie aus der Glykolyse bereit gestellt werden, wird die gesamte ATP-Versorgung des Spermiums aus den Mitochondrien bezogen (Cardullo & Baltz, 1991) Spermienmotilität und das Vorhandensein von Plasmatropfen gibt es Hinweise, dass das porzine Chromosom 5 mit männlicher Fruchtbarkeit assoziiert ist (Wiedemann et al, 2018a).…”

“…Es sind jedoch nur wenige Informationen über die Kandidatengene verfügbar, die die Reproduktionsmerkmale von Ebern beeinflussen (Liu et al, 2017). Die Ergebnisse der vorliegenden Studie zeigen, dass das porzine DNAL4-Gen als Kandidatengen für die Spermaqualität angenommen werden kann (Wiedemann et al, 2018a). Es liegt auf SSC5p15 und kodiert für eine leichte Kette axonemaler Dyneine .…”

“…Es liegt auf SSC5p15 und kodiert für eine leichte Kette axonemaler Dyneine . Ein SNP (g.1007A>G) im zweiten Intron wurde , deutet darauf hin, dass SSC5 möglicherweise mit männlicher Fruchtbarkeit assoziiert ist (Wiedemann et al, 2018a).…”