<scp>RrmA</scp>regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress

Krol, K.; Morozov, Igor Y.; Jones, Meriel G.; Wyszomirski, Tomasz; Węgleński, Piotr; Dzikowska, Agnieszka; Caddick, Mark X.

doi:10.1111/mmi.12324

Cited by 8 publications

(4 citation statements)

References 58 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, in A. nidulans AreA activity is in part modulated in response to the intracellular levels of glutamine via the regulated degradation of areA mRNA (Morozov et al ., 2000; 2001; Caddick et al ., ). Recently, it has been shown that this process depends on the RNA‐binding protein RrmA (Król et al ., ). At the protein level, the function of AreA/Nit2 is negatively regulated by binding to the nitrogen metabolite repressor NmrA under nitrogen repressing conditions, and deletion of nmrA led to partial de‐repression of nitrogen‐regulated genes (Jarai and Marzluf, ; Andrianopoulos et al ., ; Lamb et al ., 2003; 2004; Kotaka et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Michielse¹,

Pfannmüller²,

Macios

et al. 2013

Molecular Microbiology

View full text Add to dashboard Cite

SummaryNitrogen metabolite repression (NMR) in filamentous fungi is controlled by the GATA transcription factors AreA and AreB. While AreA mainly acts as a positive regulator of NMR-sensitive genes, the role of AreB is not well understood. We report the characterization of AreB and its interplay with AreA in the gibberellinproducing fungus Fusarium fujikuroi. The areB locus produces three different transcripts that each code for functional proteins fully complementing the areB deletion mutant that influence growth and secondary metabolism. However, under nitrogen repression, the AreB isoforms differ in subcellular localization indicating distinct functions under these conditions. In addition, AreA and two isoforms of AreB colocalize in the nucleus under low nitrogen, but their nuclear localization disappears under conditions of high nitrogen. Using a bimolecular fluorescence complementation (BiFC) approach we showed for the first time that one of the AreB isoforms interacts with AreA when starved of nitrogen. Cross-species complementation revealed that some AreB functions are retained between F. fujikuroi and Aspergillus nidulans while others have diverged. By comparison to other fungi where AreB was postulated to function as a negative counterpart of AreA, AreB can act as both repressor and activator of transcription in F. fujikuroi.

show abstract

Section: Introductionmentioning

confidence: 99%

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Michielse¹,

Pfannmüller²,

Macios

et al. 2013

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…RNA-binding proteins, including SwoK, FabM, PbpA, and RrmA, have been characterized in A. nidulans [ 42 - 46 ]. These RNA-binding proteins regulate fungal development and primary and secondary metabolisms.…”

Section: Introductionmentioning

confidence: 99%

Functions of PUF Family RNA-Binding Proteins in Aspergillus nidulans

Son

Jang

Park

2021

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Aspergillus nidulans is a model filamentous fungus widely used in fungal development and metabolism studies [1,2]. A. nidulans propagates through both asexual and sexual spores, but asexual spores are their primary reproductive particle [3,4]. Spores germinate to form germ tubes and a web-like mass of fungal hyphae [5]. These hyphae acquire developmental competence and undergo asexual or sexual development, depending on the environmental conditions [6,7]. For example, light can induce the formation of asexual structures-the conidiophores-but sexual fruiting bodies-the cleistothecia-are formed in dark conditions [8,9]. These reproductive processes are regulated by a myriad of transcription factors and several signaling pathways [10,11]. In asexual development, BrlA, AbaA, and WetA are central transcription factors that control mRNA expression of development-related genes [6,12]. BrlA is a C 2 H 2 zinc-finger transcription factor that controls mRNA levels of abaA and other developmental genes [13]. AbaA induces mRNA expression of three key transcription factors: WetA, VosA, and VelB [14][15][16]. These transcription factors regulate asexual spore maturation, trehalose biosynthesis, and conidial stress tolerance [15,17]. Fungal species produce various primary and secondary metabolites during development [18]. Secondary metabolites that have detrimental effects on humans are called mycotoxins [19,20]. Sterigmatocystin produced by A. nidulans is a precursor aflatoxin and is considered as a group 2B carcinogen [21]. Sterigmatocystin biosynthesis depends on a gene cluster of which the AflR transcription factor is a key regulator [22][23][24]. Other important genes involved in sterigmatocystin production include MtfA, VeA, LaeA,.RNA-binding proteins play essential roles in the posttranscriptional regulation of gene expression in eukaryotes [29][30][31]. In fungi, RNA-binding proteins participate in various fundamental processes, including development, stress responses, filamentation, and pathogenesis [32,33]. The PUF (Pumilio/Fem-3 binding factor) family of canonical RNA-binding proteins is conserved among most eukaryotic systems [34]. The Pumilio in Drosophila melanogaster and Fem-3 binding factor (FBF) in Caenorhabditis elegans are the founding members of the PUF family proteins [35]. They contain several repeated PUF domains, which interact with sequencespecific RNA elements of their target. [35]. In Ascomycota fungi, PUF proteins recognize 8-to 10-nucleotide binding elements, reducing translational efficiency or by increasing its decay [36,37]. Most of the research on the RNA targets and the biological roles of the PUF proteins have been carried out in Saccharomyces cerevisiae [35,38,39]. S. cerevisiae contains six PUF proteins involved in posttranscriptional processes, mating type switching, lifespan, thermotolerance, cell wall integrity, and mitochondrial biogenesis [35,40]. For example, PUF6 targets RNA-binding proteins are involved in RNA metabolism and posttranscriptional regulation of various fundamental biolog...

show abstract

“…2001; Krol et al . 2013). The activity of AreA is also regulated at the protein level by a co-repressor, NmrA, and co-activator TamA.…”

Section: Introductionmentioning

confidence: 99%

The role of the GATA transcription factor AreB in regulation of nitrogen and carbon metabolism in Aspergillus nidulans

Chudzicka-Ormaniec

Macios

Koper

et al. 2019

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

In Aspergillus nidulans , nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression and the nutrient starvation response. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation. To identify mechanisms coordinating nitrogen and carbon metabolism, we tested the role of AreB, previously identified as a regulator of genes involved in nitrogen metabolism. Deletion of areB has significant phenotypic effects on the utilization of specific carbon sources, confirming its role in the regulation of carbon metabolism. AreB was shown to regulate the expression of areA , tamA, creA, xprG and cpcA regulatory genes suggesting areB has a range of indirect, regulatory effects. Different isoforms of AreB are produced as a result of differential splicing and use of two promoters which are differentially regulated by carbon and nitrogen conditions. These isoforms are likely to be functionally distinct and thus contributing to the modulation of AreB activity.

show abstract

RrmAregulates the stability of specific transcripts in response to both nitrogen source and oxidative stress

Cited by 8 publications

References 58 publications

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Functions of PUF Family RNA-Binding Proteins in Aspergillus nidulans

The role of the GATA transcription factor AreB in regulation of nitrogen and carbon metabolism in Aspergillus nidulans

Contact Info

Product

Resources

About