<scp>RNAi</scp> as a management tool for the western corn rootworm, <i>Diabrotica virgifera virgifera</i>

Fishilevich, Elane; Vélez, Ana M.; Storer, Nicholas P.; Li, Huarong; Bowling, Andrew J.; Rangasamy, Murugesan; Worden, Sarah E.; Narva, Kenneth E.; Siegfried, Blair D.

doi:10.1002/ps.4324

Cited by 84 publications

(89 citation statements)

References 125 publications

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“…Transgenic plants engineered to express insect dsRNAs emerged as a potential technology after two independent groups proved the concept of applying RNAi to control agricultural insect pests (Baum et al, 2007; Mao et al, 2007). This approach has been developed to control lepidopteran, coleopteran and hemipteran agricultural pests (Katoch et al, 2013; Li et al, 2011; Paim et al, 2012), including Helicoverpa armigera in cotton (Mao et al, 2011; Mao et al, 2015; Qi et al, 2015; Chikate et al, 2016) and tobacco (Zhu et al, 2012; Xiong et al, 2013; Tian et al, 2015; Mamta, Reddy & Rajam, 2015), Diabrotica virgifera virgifera in maize (Baum et al, 2007; Fishilevich et al, 2016), Nilaparvata lugens in rice (Zha et al, 2011; Li et al, 2011; Yu et al, 2014; Qiu et al, 2016), Myzus persicae (Mao et al, 2015; Tzin et al, 2015) in Nicotiana benthamiana (Khan et al, 2013; Pitino et al, 2011) and Arabidopsis thaliana (Coleman, Pitino & Hogenhout, 2014; Li et al, 2015) and Sitobion avenae in wheat (Xu et al, 2014). However, the availability of methods that allow the screening and evaluation of candidate RNAi targets is a critical requisite for developing specific and efficient RNAi-based pest control.…”

Section: Introductionmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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RNA interference (RNAi), a gene-silencing mechanism that involves providing double-stranded RNA molecules that match a specific target gene sequence, is now widely used in functional genetic studies. The potential application of RNAi-mediated control of agricultural insect pests has rapidly become evident. The production of transgenic plants expressing dsRNA molecules that target essential insect genes could provide a means of specific gene silencing in larvae that feed on these plants, resulting in larval phenotypes that range from loss of appetite to death. In this report, we show that the tomato leafminer (Tuta absoluta), a major threat to commercial tomato production, can be targeted by RNAi. We selected two target genes (Vacuolar ATPase-A and Arginine kinase) based on the RNAi response reported for these genes in other pest species. In view of the lack of an artificial diet for T. absoluta, we used two approaches to deliver dsRNA into tomato leaflets. The first approach was based on the uptake of dsRNA by leaflets and the second was based on “in planta-induced transient gene silencing” (PITGS), a well-established method for silencing plant genes, used here for the first time to deliver in planta-transcribed dsRNA to target insect genes. Tuta absoluta larvae that fed on leaves containing dsRNA of the target genes showed an ∼60% reduction in target gene transcript accumulation, an increase in larval mortality and less leaf damage. We then generated transgenic ‘Micro-Tom’ tomato plants that expressed hairpin sequences for both genes and observed a reduction in foliar damage by T. absoluta in these plants. Our results demonstrate the feasibility of RNAi as an alternative method for controlling this critical tomato pest.

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Section: Introductionmentioning

confidence: 99%

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Camargo

Barbosa

Possignolo

et al. 2016

PeerJ

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“…37 If so, future studies on natural uptake of diet-derived sRNA should focus on invertebrates for which successful orally administered RNAi has been shown. For example, studies of diet-derived RNAi in the control of the corn rootworm 93 for agricultural uses has provided one of the best examples of natural uptake of dietary sRNAs. 37 Research in this area should focus on species for which genomic tools exist for both the plant species of the diet and the ingesting organism.…”

Section: Discussionmentioning

confidence: 99%

“…For example, various toxins work by disrupting epithelial cells in the digestive tract, such as the pore forming peptide pesticides derived from Bacillus thuringiensis. 93 In addition, one group found that introduction of a cysteine protease that reduced the peritrophic matrix resulted in increased uptake of dsRNA delivered via transgenic plants to the bollworm, 94 indicating that non-cellular barriers are important for inhibiting sRNA uptake as well.…”

Section: Do Srnas Cross the Digestive Tract Barrier?mentioning

confidence: 99%

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

Chan

Snow

2016

RNA Biology

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The putative transfer and gene regulatory activities of diet-derived small RNAs (sRNAs) in ingesting animals are still debated. The existence of natural uptake of diet-derived sRNA by invertebrate species could have significant implication for our understanding of ecological relationships and could synergize with efforts to use RNA interference (RNAi) technology in agriculture. Here, we synthesize information gathered from studies in invertebrates using natural or artificial dietary delivery of sRNA and from studies of sRNA in vertebrate animals and plants to review our current understanding of uptake and impact of natural dietderived sRNA on invertebrates. Our understanding has been influenced and sometimes confounded by the diversity of invertebrates and ingested plants studied, our limited insights into how gene expression may be modulated by dietary sRNAs at the mechanistic level, and the paucity of studies focusing directly on natural uptake of sRNA. As such, we suggest 2 strategies to investigate this phenomenon more comprehensively and thus facilitate the realization of its potentially broad impact on ecology and agriculture in the future.

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“…In Brazil, for example, studies demonstrated a high efficiency of the Bt soybean strain DAS-81419-2 (expressing two proteins) in the reduction of damage caused by A. gemmatalis, C. includens, H. virescens, and S. cosmioides during the vegetative and reproductive stages (Marques et al 2016). Recent studies have suggested the use of RNA interference as a promising alternative or complementary strategy for pest control and management (Asokan et al 2014;Kim et al 2015;Fishilevich et al 2016;Yu et al 2016;Ni et al 2017). …”

Section: Management Of Resistancementioning

confidence: 99%

Genetically modified soybean expressing insecticidal protein (Cry1Ac): Management risk and perspectives

Martins-Salles

Machado

Massochin-Pinto

et al. 2017

FACETS

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A Bt soybean has been recently developed, thus, efficiently regulating the populations of major lepidopteran pests. However, in other cases, these benefits have been reduced or lost because of the rapid evolution of pest resistance to the Bt toxins in transgenic crops. When pest populations are exposed to Bt crops and to refuges (non-Bt plants), the evolution of resistance is governed by the fitness of resistant individuals relative to susceptible individuals for both the presence and absence of Bt toxins. One major ecological concern regarding the biosafety of Bt crops on the environment is their potential effects on non-target organisms, especially predators and parasitoids that play an important role in pest control. This information is important for supporting insect resistance management (IRM) programs and for improving agricultural practices in a crop production system with Bt plants. Before the use of Bt plants for insect pest control in Brazil is adopted, IRM programs should be established to ensure the sustainability of this technology for integrated pest management (IPM). This review presents data on Bt soybean and lepidopteran pests as well as on the importance of natural enemies as a form of biological control, and applications for IPM and IRM.

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RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera

Cited by 84 publications

References 125 publications

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

RNA interference as a gene silencing tool to controlTuta absolutain tomato (Solanum lycopersicum)

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

Genetically modified soybean expressing insecticidal protein (Cry1Ac): Management risk and perspectives

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