<scp>R</scp>aman Spectroscopy for the Study of Molecular Order, Thermodynamics, and Solid–Liquid Transitions in Triacylglycerols

Silva, Éric Da; Rousseau, Dérick

doi:10.1002/0470027320.s8947

Cited by 3 publications

(4 citation statements)

References 46 publications

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“…In Raman spectra, the ester carbonyl stretching region can provide an insight into the lipid phase organization (47,48). Triglycerides have three polymorphic forms in the solid-like ordered phase: a, b 0 , and b (49).…”

Section: C¼o Stretching Bandmentioning

confidence: 99%

Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy

Okotrub

Mokrousova

Amstislavsky

et al. 2018

Biophysical Journal

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Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid droplets. We apply Raman spectroscopy to investigate freezing of lipid droplets inside cumulus-oocyte complexes, mature oocytes, and early embryos of a domestic cat. Raman spectroscopy allows one to characterize the degree of lipid unsaturation, the lipid phase transition from the liquid-like disordered to solid-like ordered state, and the triglyceride polymorphic state. For all cells examined, the average degree of lipid unsaturation is estimated as $1.3 (with 520% deviation) double bonds per acyl chain. The onset of the lipid phase transition occurs in a temperature range from À10 to þ4 C and does not depend on the cell type. Lipid droplets in cumulus-oocyte complexes are found to undergo abrupt lipid crystallization shifted in temperature from the ordering of the lipid conformational state. In the case of mature oocytes and early embryos obtained in vitro, the lipid crystallization is broadened. In the frozen state, lipid droplets inside cumulus-oocyte complexes have a higher content of triglyceride polymorphic b and b 0 phases than estimated for mature oocytes and early embryos. For the first time, to our knowledge, the temperature evolution of the phase state of lipid droplets is examined. Raman spectroscopy is proved to be a promising tool for in situ monitoring of the lipid phase state in a single embryo/oocyte during its freezing.

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Section: C¼o Stretching Bandmentioning

confidence: 99%

Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy

Okotrub

Mokrousova

Amstislavsky

et al. 2018

Biophysical Journal

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“…However, spectral changes are observed that support the changes in the polarity of the local environment around the ester carbonyl group and the increased conformational order in the hydrocarbon chains. For example, the changes observed in the regions from 1000–1150 cm −1 and 1400–1500 cm −1 are diagnostic of improved alkyl chain packing arrangements, as they reflect concomitant increases in both hydrocarbon chain mobility and the relative content of trans/gauche conformers [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma

et al. 2022

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Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.

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“…In the Raman spectra, ester carbonyl stretching region can provide the insight into the lipid phase organization (27,28). Triglycerides have three polymorphic forms in solid-like ordered phase: α, β′, and β (55).…”

Section: Resultsmentioning

confidence: 99%

“…Freezing membranes and LDs undergo a lipid phase transition (LPT) into solid-like ordered phases (25,26). Triglycerides contained in the LDs can exist in several polymorphic solid-like states with different phase stability and melting temperatures (26-28). Two different order parameters can be used to characterize the lipid state during freezing.…”

Section: Introductionmentioning

confidence: 99%

Lipid droplet phase transition in freezing cat embryos and oocytes probed by Raman spectroscopy

Okotrub

Mokrousova

Amstislavsky

et al. 2018

Preprint

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Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One challenging limitation of this technology is the cell damage during freezing associated with the intracellular lipid droplets. We exploit a Raman spectroscopy to investigate the freezing of cumulus-oocyte complexes, mature oocytes and early embryos of a domestic cat. All these cells are rich in lipids. The degree of lipid unsaturation, lipid phase transition from liquid-like disordered to solid-like ordered state (main transition) and triglyceride polymorphic state are studied. For all cells examined, the average degree of lipid unsaturation is estimated about 1.3 (with ±20 % deviation) double bonds per acyl chain. The onset of the main lipid phase transition occurs in a temperature range from −10 to +4 °C and does not depend significantly on the cell type. It is found that lipid droplets in cumulus-oocyte complexes undergo an abrupt lipid crystallization, which not completely correlate with the ordering of lipid molecule acyl chains. In the case of mature oocytes and early embryos obtained in vitro from cumulus-oocyte complexes, the lipid phase transition is broadened. In frozen state lipid droplets inside the cumulus-oocyte complexes have higher content of triglyceride polymorphic β and β′ phases (∼66%) than it is estimated for the mature oocytes and the early embryos (∼50%). For the first time, to our knowledge, temperature evolution of lipid droplets phase state is examined. Raman spectroscopy is proved as a prospective tool for in situ monitoring of lipid phase state in single embryo/oocyte during freezing.

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Raman Spectroscopy for the Study of Molecular Order, Thermodynamics, and Solid–Liquid Transitions in Triacylglycerols

Cited by 3 publications

References 46 publications

Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy

Lipid Droplet Phase Transition in Freezing Cat Embryos and Oocytes Probed by Raman Spectroscopy

Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma

Lipid droplet phase transition in freezing cat embryos and oocytes probed by Raman spectroscopy

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