<scp>Poly‐L</scp>‐lactide scaffolds with super pores obtained by freeze‐extraction method

Budnicka, Monika; Kołbuk, Dorota; Ruśkowski, Paweł; Gadomska‐Gajadhur, Agnieszka

doi:10.1002/jbm.b.34642

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Introducing the porophor (POR samples) affects the scaffold’s morphology, producing pores of more regular shapes and increasing their interconnectivity and roughness of surfaces [ 26 , 27 ]. The pores in both systems are 100–400 µm and optimal for bone regeneration [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone

Kołakowska

Kołbuk

Chwojnowski

et al. 2023

JFB

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An innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancellous bone (pore size 100–400 µm, open porosity 94%). The high absorbability of the implants will enable their use as platelet-rich plasma (PRP) carriers in future medical devices. Surface modification by dipping enabled the deposition of the hydrophilic chitosan (CS) layer, maintaining good bone tissue properties and high absorbability (850% dry weight). Introducing CS increases surface roughness and causes local changes in surface free energy, promoting bone cell adhesion. Through this research, we have developed a new and original method of low-temperature modification of PLA substitutes with chitosan. This method uses non-toxic reagents that do not cause changes in the structure of the PLA matrix. The obtained bone substitutes are characterised by exceptionally high hydrophilicity and morphology similar to spongy bone. In vitro studies were performed to analyse the effect of morphology and chitosan on cellular viability. Substitutes with properties similar to those of cancellous bone and which promote bone cell growth were obtained.

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Section: Discussionmentioning

confidence: 99%

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone

Kołakowska

Kołbuk

Chwojnowski

et al. 2023

JFB

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“…Biological studies were performed using mouse fibroblasts. Tests were done using a previously developed procedure [21,22], which is briefly described below.…”

Section: Cellular Viabilitymentioning

confidence: 99%

Poly(Glycerol Succinate) as an Eco-Friendly Component of PLLA and PLCL Fibres towards Medical Applications

et al. 2020

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This study was conducted as a first step in obtaining eco-friendly fibres for medical applications using a synthesised oligomer poly(glycerol succinate) (PGSu) as an additive for synthetic poly(L-lactic acid) (PLLA) and poly (L-lactide-co-caprolactone) (PLCL). The effects of the oligomer on the structure formation, morphology, crystallisation behaviour, and mechanical properties of electrospun bicomponent fibres were investigated. Nonwovens were investigated by means of scanning electron microscopy (SEM), wide angle X-ray scattering (WAXS), differential scanning calorimetry (DSC), and mechanical testing. The molecular structure of PLLA fibres is influenced by the presence of PGSu mainly acting as an enhancer of molecular orientation. In the case of semicrystalline PLCL, chain mobility was enhanced by the presence of PGSu molecules, and the crystallinity of bicomponent fibres increased in relation to that of pure PLCL. The mechanical properties of bicomponent fibres were influenced by the level of PGSu present and the extent of crystal formation of the main component. An in vitro study conducted using L929 cells confirmed the biocompatible character of all bicomponent fibres.

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“…Lactide, caprolactone, and glycolide are the commonly used monomers to synthesise aliphatic polyesters and copolymers that are applied in tissue engineering [4]. Polymers can be manufactured using various techniques, e.g., electrospinning [5], 3D printing [6], and freeze-extraction [7]. Their bulk properties can be tailored via various methods, e.g., copolymerization [8], controlled crystallization [9], or the use of additives [10].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Three Approaches to Fibre’s Surface Functionalization

Dulnik

Jeznach

2022

JFB

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Polyester-based scaffolds are of research interest for the regeneration of a wide spectrum of tissues. However, there is a need to improve scaffold wettability and introduce bioactivity. Surface modification is a widely studied approach for improving scaffold performance and maintaining appropriate bulk properties. In this study, three methods to functionalize the surface of the poly(lactide-co-ε-caprolactone) PLCL fibres using gelatin immobilisation were compared. Hydrolysis, oxygen plasma treatment, and aminolysis were chosen as activation methods to introduce carboxyl (-COOH) and amino (-NH2) functional groups on the surface before gelatin immobilisation. To covalently attach the gelatin, carbodiimide coupling was chosen for hydrolysed and plasma-treated materials, and glutaraldehyde crosslinking was used in the case of the aminolysed samples. Materials after physical entrapment of gelatin and immobilisation using carbodiimide coupling without previous activation were prepared as controls. The difference in gelatin amount on the surface, impact on the fibres morphology, molecular weight, and mechanical properties were observed depending on the type of modification and applied parameters of activation. It was shown that hydrolysis influences the surface of the material the most, whereas plasma treatment and aminolysis have an effect on the whole volume of the material. Despite this difference, bulk mechanical properties were affected for all the approaches. All materials were completely hydrophilic after functionalization. Cytotoxicity was not recognized for any of the samples. Gelatin immobilisation resulted in improved L929 cell morphology with the best effect for samples activated with hydrolysis and plasma treatment. Our study indicates that the use of any surface activation method should be limited to the lowest concentration/reaction time that enables subsequent satisfactory functionalization and the decision should be based on a specific function that the final scaffold material has to perform.

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Poly‐L‐lactide scaffolds with super pores obtained by freeze‐extraction method

Cited by 5 publications

References 33 publications

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone

Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone

Poly(Glycerol Succinate) as an Eco-Friendly Component of PLLA and PLCL Fibres towards Medical Applications

A Comparative Study of Three Approaches to Fibre’s Surface Functionalization

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