<scp>PM</scp> 7/28 (2) <i>Synchytrium endobioticum</i>

doi:10.1111/epp.12441

Cited by 29 publications

(5 citation statements)

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“…Late blight was tested using the widely accepted laboratory and eld trials of arti cial infection by separate tubers and leaves of the Phytophthora infenstans (Mont) de Bary fungal inoculum, created by the Institute for Potato Study NAAS (Cherednychenko et al, 2021;EPPO Standard, 2017). The defeating degree was de ned using 9 points scale: 9 -very high resistance (symptoms of infected absent); 7 -relatively high resistance (infected tissue takes from 10% to 25 % surface and tubers cut); 5 -mid resistance (infected from 25% to 50%); 3 -low resistance (infected from 50% to 75%); 1 -very low resistance (infected more than 75%).…”

Section: Methodsmentioning

confidence: 99%

Cultivar resistance of wart causative agents and late blight pathogen in potato breeding in Lithuania

Asakaviciute,

Zelya,

Kacergius

et al. 2023

Preprint

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There were chosen 6 potato varieties of Lithuanian breeding: ‘VB Venta’, ‘Goda’, ‘VB Liepa’, ‘VB Meda’, ‘VB Rasа’ and ‘VB Aista’ by the research results during 2020-2021. Three potato varieties ‘VB Goda’, ‘VB Liepa’ and ‘VB Meda’ chose with high resistance degree to Phytophthora infenstans (Mont) de Bary. These potato varieties are recommended for use as parent forms for crossing and for implementation in potato disease sources. Microsatellite simple repetitive sequence (PPS) markers are commonly used in various breeding and genetic studies because of their abundance, repetition, and extensive genome coverage. According to the results, it can be stated that the Lithuanian potato varieties have sufficient genetic differences. This also leads to different agro-morphological characteristics; however, greater genetic variation should be sought to broaden the range of breed characteristics.

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Section: Methodsmentioning

confidence: 99%

Cultivar resistance of wart causative agents and late blight pathogen in potato breeding in Lithuania

Asakaviciute,

Zelya,

Kacergius

et al. 2023

Preprint

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“…Resting spores were isolated from 200 g compost, which serve as inoculum for bio-assays, from the NPPO-NL S. endobioticum collection with a zonal centrifuge as described previously [5]. For the Canadian material, resting spores were collected in sieves, washed, and centrifuged, as described previously [8][9][10].…”

Section: Selection Of Samplesmentioning

confidence: 99%

A target enrichment approach for enhanced recovery of Synchytrium endobioticum nuclear genome sequences

Nguyen,

Ponomareva,

Dadej

et al. 2024

PLoS ONE

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Potato wart disease is caused by the obligate fungal pathogen Synchytrium endobioticum. DNA extraction from compost, purified spores and crude wart tissue derived from tuber galls of infected potatoes often results in low S. endobioticum DNA concentration or highly contaminated with DNA coming from other microorganisms and the potato host. Therefore, Illumina sequencing of these samples generally results in suboptimal recovery of the nuclear genome sequences of S. endobioticum. A hybridization-based target enrichment protocol was developed to strongly enhance the recovery of S. endobioticum DNA while off-target organisms DNA remains uncaptured. The design strategy involved creating a set of 180,000 molecular baits targeting both gene and non-gene regions of S. endobioticum. The baits were applied to whole genome amplified DNA samples of various S. endobioticum pathotypes (races) in compost, from purified spores and crude wart tissue samples. This was followed by Illumina sequencing and bioinformatic analyses. Compared to non-enriched samples, target enriched samples: 1) showed a significant increase in the proportion of sequenced bases mapped to the S. endobioticum nuclear genome, especially for crude wart tissue samples; 2) yielded sequencing data with higher and better nuclear genome coverage; 3) biased genome assembly towards S. endobioticum sequences, yielding smaller assembly sizes but higher representation of putative S. endobioticum contigs; 4) showed an increase in the number of S. endobioticum genes detected in the genome assemblies. Our hybridization-based target enrichment protocol offers a valuable tool for enhancing genome sequencing and NGS-based molecular detection of S. endobioticum, especially in difficult samples.

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“…The potato plant is a host for a noxious obligate pathogen, namely the zoosporic chytrid fungus Synchytrium endobioticum (Schilbersky) Percival 1909, causing the so-called potato wart disease, which is subject to quarantine regulations in many countries of the world [3][4][5][6]. According to the data of the European and Mediterranean Plant Protection Organization (EPPO), as of January 2018 S. endobioticum is commonly found in 34 countries, including the Falkland Islands (Great Britain) and the Faroe Islands (Denmark) [7].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2018

Agric. sci. pract.

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Aim.To evaluate potato breeding material for resistance to pathotypes of Synchytrium endobioticum (Schilbersky) Percival (1909) known to be present in Ukraine (pathotypes 1(D 1 ), 11, 13, 18 and 22); to identify resistant registered and potential varieties for the usage in the national wart disease eradication programs and to recommend these selected (potential and registered) potato varieties for the breeding program targeted on the development of multiple resistance against pathotypes of S. endobioticum present in Ukraine. Methods. Evaluation of the potato breeding material and registered potato varieties for the resistance against common pathotype 1 (D 1 ) and four aggressive pathotypes of S. endobioticum (pathotypes 11, 13, 18 and 22) in climatic chamber and greenhouse tests of Ukrainian Scienti¿ c Research Plant Quarantine Station of Institute of Plant Protection NAAS (Boyany, Ukraine) following the Spieckermann and Glynne-Lemmerzahl methods (EPPO Standard PM7/28 (2)). Field trials on naturally infected soils were conducted according to standard methods adapted to national requirements in the area of Chernivtsi, Zakarpattia and Ivano-Frankivsk regions. Results. 3,736 samples of potato breeding material from six breeding institutions of Ukraine were tested for resistance against S. endobioticum during 2011-2017 in the western region of the country. Among all samples tested, 3,389 were identi¿ ed as resistant to the widely spread pathotype 1 in the preliminary climatic chamber and greenhouse tests, and 130 of them proved to be resistant under ¿ eld conditions. Five out of 41 Ukrainian registered potato varieties (Bazys, Hlazurna, Solokha, Bozhedar and Santarka) were found to be resistant to all 5 pathotypes tested (1 (D 1 ), 11, 13, 18 and 22). Conclusions. The 130 samples of potato breeding material (which were found to be resistant against the common pathotype 1 of S. endobioticum in the laboratory, greenhouse as well as in the ¿ eld trials) were recommended for the state variety registration and further usage in an eradication program to localize potato wart outbreaks of the western part of Ukraine. The screening tests revealed that the national breeding program targeted on resistance against S. endobioticum pathotype 11 was the most effective (49 % of samples tested proved to be resistant against this pathotype), whereas it was the least effective against pathotype 18, namely only 30 % of samples resistant. It was speculated that such a dissimilarity may be related to the differences in the genetic material used in the breeding process at various institutions, and which may be the subject of further analysis in order to improve the results of breeding programs. The already registered potato varieties Bazys, Hlazurna, Solokha, Bozhedar and Santarka which were found to have a multiple resistance to common pathotype 1 and four local aggressive pathotypes of S. endobioticum (11, 13, 18 and 22) were recommended for use in the breeding process as sources of resistance and also for the eradication programs in ...

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PM 7/28 (2) Synchytrium endobioticum

Abstract: Specific scope This Standard describes a diagnostic protocol for Synchytrium endobioticum. This Standard should be used in conjunction with PM 7/76 Use of EPPO diagnostic protocols. Specific approval and amendment Approved in 2003‐09. Revision approved in 2017‐06.

Cited by 29 publications

References 16 publications

Cultivar resistance of wart causative agents and late blight pathogen in potato breeding in Lithuania

Cultivar resistance of wart causative agents and late blight pathogen in potato breeding in Lithuania

A target enrichment approach for enhanced recovery of Synchytrium endobioticum nuclear genome sequences

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