<scp>pH</scp> and effects on <i><scp>S</scp>treptococcus mutans</i> growth of denture adhesives: an <i>in vitro</i> study

Chen, Fengying; Mao, Tiantian; Cheng, Xiang‐Rong

doi:10.1111/ger.12013

Cited by 3 publications

(6 citation statements)

References 22 publications

(36 reference statements)

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“…16 The results point to significant modifications on the pH of the culture media following the addition of the denture adhesives. Previous reports have described changes in pH after different periods of incubation, 5,16 with a reduction below 5.0 in one study. 17 However, de Gomes et al described different degrees of cytotoxicity without changes in the pH of the culture medium.…”

Section: Discussionmentioning

confidence: 81%

“…Moreover, the product is easily available at any pharmacy, with no need for a prescription and at an affordable price 4 . However, the effects that this type of product can have on oral mucosa cells have not been extensively tested, despite the fact that the substance is in contact with the oral mucosa many hours a day, or could even be ingested by the user 5,6 …”

mentioning

confidence: 99%

“…4 However, the effects that this type of product can have on oral mucosa cells have not been extensively tested, despite the fact that the substance is in contact with the oral mucosa many hours a day, or could even be ingested by the user. 5,6 Denture adhesives have three main components: those responsible for adhesion, those which act as antimicrobial agents and others that act as fillers. The adhesive substances include karaya gum, gelatin, pectin, methylcellulose, hydroxymethyl cellulose, and carboxymethyl cellulose sodium salt.…”

mentioning

confidence: 99%

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Are Denture Adhesives Safe for Oral Cells?

López‐García

Pecci-Lloret

García‐Bernal

et al. 2020

Journal of Prosthodontics

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Purpose To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Poligrip Flavour Free Fixative Cream, Fixodent Pro Duo Protection, Novafix cream, FittyDent, Polident Total Action, and Fixodent Pro Plus Duo Protection. Material and Methods Eluates of denture adhesives were brought into contact with human gingival cells and compared to untreated cells (w/o any dental adhesive elute). Cell toxicity was assessed by measuring cell viability (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) (MTT) assays), cell morphology (immunofluorescence assays), induction of apoptosis/necrosis and production of reactive oxygen species (ROS) (flow cytometry assays). In addition, the pH of each sample was determined. Data were analyzed using one‐way analysis of variance (ANOVA) followed by Dunnett's multiple comparisons test. Results All denture adhesives tested led to a reduction in pH, especially Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection. The cell viability assays showed that Fixodent Pro Duo Protection (1:1 72 hours, p = 3.04 × 10−6; 1:2 72 hours, p = 2.07 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) and Fixodent Pro Plus Duo Protection (1:1 72 hours, p = 2.01 × 10−6; 1:2 72 hours, p = 3.03 × 10−6; 1:4 72 hours, p = 2.02 × 10−6) significantly decreased cell viability at all dilutions. Compared to the control group and the rest of the adhesives, Poligrip Flavour Free Fixative Cream (PFF 1:1 72 hours, p = 2.24 × 10−6; 1:2 72 hours, p = 2.44 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) showed a significantly higher cell viability score at all dilutions. Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection, both adhesives containing zinc salts in their composition, were responsible for necrosis, and the number of cells was much reduced, with aberrant morphology and pyknotic nucleus. Finally, Fixodent (1:2, p = 2.04 × 10−6, 1:4, p = 0.00036; 1:2, p = 8.82 × 10−6, 1:4, p = 2.30 × 10−6) products significantly promoted ROS production in gingival cells. Conclusions The results suggest that denture adhesives containing zinc in their composition could be responsible of the decrease of cell viability, ROS production, aberrant cell morphology, and induction of apoptosis and cell death. However, other possible additional cytotoxic factors must be considered. Thus, more studies are necessary to confirm this hypothesis.

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Section: Discussionmentioning

confidence: 81%

mentioning

confidence: 99%

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confidence: 99%

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Are Denture Adhesives Safe for Oral Cells?

López‐García

Pecci-Lloret

García‐Bernal

et al. 2020

Journal of Prosthodontics

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“…Owing to these features concerted with its survivability, natural bioactive substances such as sugar-alcohol, antocyanine, and specific sugars have been focused as potent antimicrobial materials. It has been implicated that extracellular water-insoluble polysaccharide from dietary sugar produced by dextransucrase (DSase) and its adhesion to dental enamel are known to the most clinically important biological abilities of S. mutans (Chen et al 2012;Decker et al 2014). However, the mechanisms under which this cariogenic pathogen can survive or proliferate under in such environmental conditions are so far largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Significant persistence of S. mutans under the acidic environment encourages the proliferation of S. mutans including other acidogenic bacteria, resulting in their ability to survive at a low-pH condition. Upon changing to the low-pH condition, the initiation of the dental caries starts under the surface of teeth covered with biofilm (Chen et al 2012;Decker et al 2014). Therefore, the potential acidification of dental biofilms and later cavity formations can be decreased by eliminating the adherence of S. mutans to the surface of teeth or dental biofilms.…”

Section: Introductionmentioning

confidence: 99%

Comparison of inhibitory activity of bioactive molecules on the dextransucrase from Streptococcus mutans

Lee

Park

2015

Appl Microbiol Biotechnol

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The effect of chitosan with different molecular weights and other natural substances on dextransucrase (DSase) activity from a representative oral pathogen Streptococcus mutans was elucidated. Among other bioactive substances, amino-monosaccharides such as glucosamine, mannosamine, and galactosamine exerted the enzyme inhibitory activity over 95% of DSase. The specified hydrolysates derived from the hydrolysis of high molecular weight chitosan (HMWC) designated to CTSN, CTSN-P, CTSN-B, and CTSN-S with different molecular weights ranging from 3 to 8 kDa showed the similar inhibitory activity toward DSase. Also, the hyaluronic acid (MW 8.9 kDa), sulfated chitin, and amino-monosaccharides demonstrated the significant activity, CTSN, CTSN-P, CTSN-B, and CTSN-S are of potent bioactive substances that can be prepared in the cheapest way compared with other molecules tested available for antibacterial agent useful for human oral health.

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Changes in denture retention with denture adhesives and oral moisturizers for the oral cavity: an in vitro study

et al. 2021

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Background It is difficult to maintain complete dentures during meals in place. This in vitro study aimed to assess changes in denture retention between rest and function using denture adhesives and oral moisturizers in an oral cavity model. Methods The following test samples were applied between the palatal plate and the edentulous jaw ridge model: denture adhesive, denture adhesive for dry mouth, oral moisturizer, and denture moisturizer. The retentive force was measured under two conditions: at rest while immersed in water and during function with a 2.5-kg load applied. The plate was pulled perpendicular to the occlusal plane and the retentive force was measured using a digital force gauge. Results Under dry conditions, denture adhesive for dry mouth and oral moisturizer had a significantly higher retentive force than denture adhesive and denture moisturizer. After 30 min of immersion in water, the retentive force of the denture adhesive increased while that of the oral moisturizer decreased. After 30 min of function, the retentive force of the denture adhesive and denture adhesive for dry mouth remained high, while that of the oral moisturizer and denture moisturizer significantly decreased. Between rest and function, the retentive force of the denture adhesive and denture adhesive for dry mouth was high, and that of the oral moisturizer was low. Conclusions Immediately after use, denture adhesive for dry mouth exhibited high retentive force, but retention gradually decreased due to its water content.

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pH and effects on Streptococcus mutans growth of denture adhesives: an in vitro study

Abstract: Some adhesives produce a pH below the critical pH of hydroxyapatite and may not be suitable for patients with natural teeth. None of the tested adhesives significantly affect S. mutans growth.

Cited by 3 publications

References 22 publications

Are Denture Adhesives Safe for Oral Cells?

Are Denture Adhesives Safe for Oral Cells?

Comparison of inhibitory activity of bioactive molecules on the dextransucrase from Streptococcus mutans

Changes in denture retention with denture adhesives and oral moisturizers for the oral cavity: an in vitro study

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