<scp>PCR</scp> for antigen receptor rearrangement (<scp>PARR</scp>) in equine veterinary medicine

Hollis, A. R.

doi:10.1111/eve.12857

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…The accurate distinction between reactive and neoplastic lymphoid proliferations and the differentiation of T- and B-lymphocytes can be challenging, all the more so since in horses, the most frequent cutaneous malignant lymphomas are T-cell-rich B-cell lymphomas [ 9 , 10 , 20 ]. Molecular clonality assays, assessing clonality of T- or B-cells through PARR can effectively complete clinical, morphologic, and immunophenotypic assessments [ 21 , 22 ]. Clonality assays are species-specific and in veterinary medicine, are currently only available for dogs, cats, and horses [ 21 – 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Molecular clonality assays, assessing clonality of T- or B-cells through PARR can effectively complete clinical, morphologic, and immunophenotypic assessments [ 21 , 22 ]. Clonality assays are species-specific and in veterinary medicine, are currently only available for dogs, cats, and horses [ 21 – 23 ]. In the present case, the test was performed using primer sets for equine PARR on formalin-fixed paraffin-embedded tissue.…”

Section: Discussionmentioning

confidence: 99%

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Cutaneous epitheliotropic T-cell lymphoma in a donkey – a case report

Kondratjeva

Julien²,

Coutelier

et al. 2022

BMC Vet Res

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Background Cutaneous epitheliotropic T-cell lymphoma is a malignant tumour of the skin already reported in humans, dogs, cats, horses, and other species, but not previously in donkeys. The standard diagnosis is based on clinical, morphological and immunophenotypic data. Differentiation of malignant versus benign proliferation of lymphocytes is crucial; in ambiguous cases T-cell receptor gamma (TRG) molecular clonality should be tested. In the present paper, we report a case of mycosis fungoides diagnosed in a donkey whose diagnosis was based on clinical, histological and immunohistochemical aspects and a positive TRG clonality test. Case presentation A twenty-five-year-old donkey gelding was referred with a mildly pruritic, generalised and severe exfoliative dermatosis. Otherwise, the animal was clinically healthy, though mildly underweight. Dermatological examination revealed severe generalised alopecic and exfoliative dermatitis, occasionally eroded, with high number of large, thin, greyish scales. All mucocutaneous junctions except the hoofs were affected. Ectoparasites and dermatophytes were ruled out. The complete blood count and blood smear evaluation revealed mild normocytic normochromic anemia. The biochemistry panel showed mild hyperproteinemia with albumin within the normal range. Protein electrophoresis showed moderate polyclonal hypergammaglobulinemia. Histological findings were characterised by interface dermatitis with massive exocytosis in the epidermis of a homogenous population of lymphoid cells showing atypia. Clusters of neoplastic cells were present within the epidermis forming Pautrier “microabscesses”. These findings are consistent with cutaneous epitheliotropic lymphoma. Immunohistochemical staining revealed uniform labelling of the neoplastic cells for CD3, and lack of expression of CD20 (a B cell lineage associated marker). Molecular clonality PCR (PARR) was performed using equine TRG primers; this revealed a clonal rearrangement in a heavy polyclonal background. Transmission electronic microscopy showed multiple lymphocytes with convoluted or cerebriform nuclei. Conclusions This case report provides the first evidence of clinical, histopathological, immunophenotypic features, electron microscopy findings and molecular analysis of a cutaneous epitheliotropic T-cell lymphoma (mycosis fungoides) in a donkey. Our observations suggest that cutaneous T-cell lymphoma should be included in the differential diagnoses of exfoliative dermatitis, even those progressing in a chronic pattern and/or with few or no pruritus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cutaneous epitheliotropic T-cell lymphoma in a donkey – a case report

Kondratjeva

Julien²,

Coutelier

et al. 2022

BMC Vet Res

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“…Sie sollte nicht für sich allein, sondern als zusätzliches Puzzleteil genutzt werden. In der Humanmedizin dient die Untersuchung primär zum Staging und Monitoring [18].…”

Section: Anamneseunclassified

Der klinische Fall: Differenzialdiagnosen von Angesichtsschwellungen beim Pferd

Geiersberger,

Rohwerder

2023

pferde spiegel

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PCR for antigen receptor rearrangement (PARR) in equine veterinary medicine

Cited by 2 publications

References 10 publications

Cutaneous epitheliotropic T-cell lymphoma in a donkey – a case report

Cutaneous epitheliotropic T-cell lymphoma in a donkey – a case report

Der klinische Fall: Differenzialdiagnosen von Angesichtsschwellungen beim Pferd

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