PAMAM Dendrimer Conjugated with Cell‐penetrating Peptide‐derived Oligopeptides for Enhanced Cell Uptake and Gene Delivery

Superior gene transfection efficiency in triple negative breast cancer by RAFT-mediated amino acid-based cationic diblock copolymers

“…17,18 To account for these discrepancies, analogs of cationic polymers with natural moieties, such as amino acids, fatty acids, and carbohydrates, are prepared, which are reported to increase polyplex stability and, therefore, facilitate cellular uptake. 19,20 Owing to advancements in the field of polymer chemistry, a library of polymers with controlled molecular weights, dimensions and versatile molecular architectures can be developed via RAFT polymerization. RAFT polymerization is a flexible and expedient means for preparing welldefined telechelic polymers while tolerating various solvents, reaction conditions and functionalities.…”

Theoretical–experimental prediction of the selectivity between polyamidoamine dendrimers and bioactive peptides derived from amaranth seeds

“…Based on the synthesized molecules, the available amino acids and the theoretical dendrimer–amino acid interaction, the best energy interaction between methionine, tyrosine, valine and arginine was calculated quantitatively, based on previously reported experimental studies. 57,58 We can see that the dendrimer shows some selectivity toward amino acids that ionize and form hydrogen bonds with higher interaction strength. These studies allowed us to predict the type of peptides that can be selectively separated from a complex mixture resulting from the hydrolysis of proteins derived from amaranth seeds or oligopeptides of a different nature.…”

“…This example provides a graphic, precise, and specific view of the interaction site and the coupling energy of the dendrimer-peptide complex. 57,68…”

Functionalized PAMAM constructed nanosystems for biomacromolecule delivery

“…The transfection efficiencies of PAMAM G4-KFF and PAMAM G4-FFK were improved significantly when compared with the gold-standard transfection reagent polyethyleneimine (PEI 25 kDa). 148 Although the delivery mechanisms guided by CPPs are still not clear, 149 the applications of CPPs for biomacromolecule delivery have been extensively investigated. Interestingly, the sequences of CPPs usually contain positively charged amino acid residues such as arginine and lysine.…”