P‐selectin, E‐selectin, and CD40L over time in chronic hemodialysis patients

Abstract

The aim of this study was to measure P-selectin, E-selectin, and CD-4L levels over time in chronic hemodialysis (HD) patients. Thirty stable patients with end-stage renal failure undergoing chronic HD were included in the study. Blood samples were obtained before HD for measurement of P-selectin, E-selectin, and CD-40L. Measurements were performed at month 0 (T0), 3 (T2), 8 (T3), and 13 (T4). The levels of P-selectin, E-selectin, and CD40L were also analyzed according to the occurrence of cardiovascular disease (CVD) and to CVD-related mortality. The levels of CD40L and P-selectin changed significantly over time, decreasing at month 3 and 6 and returning at the T0 levels at month 13. Conversely, E-selectin levels did not. The levels of CD40L, P-selectin and E-selectin over time did not differ significantly between patients with age ≤ 65 or > 65 years, between patients with or without CVD, or between patients who died or who survived during the follow-up. In end-stage renal failure patients undergoing chronic HD, CD40L and P-selectin, but not E-selectin, showed a transient decrease over time, and the serum levels of these molecules were not associated with CVD or with CVD-related mortality.

Efficacy of different hemodialysis methods on dendritic cell marker CD40 and CD80 and platelet activation marker CD62P and P10 in patients with chronic renal failure

“…CRF is a chronic condition characterized by inflammation, and hypercoagulability, meaning patients will have increased levels of procoagulant and inflammatory markers . In CRF patients that had undergone HD, a transient decrease in CD40L and CD62P was detected . CD62P, as an indicator of platelet degranulation when expressed on platelet surface, plays a mediate role in the interactions of platelet and leukocyte cells, which can stimulate the production of chemoattractants and growth factors .…”

“…38 In CRF patients that had undergone HD, a transient decrease in CD40L and CD62P was detected. 39 CD62P, as an indicator of platelet degranulation when expressed on platelet surface, plays a mediate role in the interactions of platelet and leukocyte cells, which can stimulate the production of chemoattractants and growth factors. 16 Targeting CD40 enhances the delivery of tumor antigen and simultaneously stimulates the activation of antigen-presenting cells with CD40-CD40L interaction, 40 which induces the maturation and activation of dendritic cells (DC), thereby promoting immune response.…”

Soluble adhesion molecules as markers of native arteriovenous fistula thrombosis in children on uremia

“…Serum levels of the adhesion molecule-1 sVCAM-1, sICAM-1, sE-selectin and sP-selectin were assayed using commercially available ELISA Kit, which was performed in the laboratories of clinical and chemical pathology unit, of NRC in Egypt. The serum concentration of sP-selectin was measured with ELISA kit (Booster Biological Technology Co, Fremont, California, USA) with sensitivity less than 5 pg/ml [28] and serum sVCAM-1 was performed (RayBiotech, Inc, Norcross, Georgia, USA) with sensitivity less than 0.3 ng/ ml [29]. Human serum sE-selectin and sICAM-1 was performed by Booster Biological Technology Co, Inc. (Lot EK0501) [30,31].…”

Effect of a Virtual Reality Exercise on Patients Undergoing Haemodialysis: A Randomised Controlled Clinical Trial Research Protocol

“…In CKD, the most widely studied adhesion molecules associated with inflammation, cardiovascular risk, and mortality are intercellular adhesion molecules-1 (ICAM-1) and vascular adhesion molecules-1 (VCAM-1). Increased levels of these markers predict overall mortality and cardiovascular mortality in patients with CKD not undergoing HD and in patients undergoing HD [10].…”