NMR: Proteins

Abstract

Solution NMR methods for determining three‐dimensional, atomic resolution structures and dynamic properties of biologic macromolecules are well established. Currently, an ever‐increasing number of experimentally solved protein NMR structures is available and contributes to our understanding of biology. The development of novel NMR approaches, labeling techniques, and calculational algorithms allows the study of larger and more complex systems. Here I summarize the basic NMR approaches, discuss labeling and assignment strategies, and provide examples for their uses.