<scp>MdVQ10</scp> promotes wound‐triggered leaf senescence in association with <scp>MdWRKY75</scp> and undergoes antagonistic modulation of <scp>MdCML15</scp> and <scp>MdJAZs</scp> in apple

Zhang, Xiaowei; Xu, Rui; Liu, Yankai; You, Chun‐Xiang; An, Jianping

doi:10.1111/tpj.16341

Cited by 15 publications

(4 citation statements)

References 76 publications

(123 reference statements)

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“…Overexpression of ZmVQ52 accelerated leaf senescence in Arabidopsis [ 23 ]. MdVQ10 accelerates wound-induced leaf senescence by cooperating with MdWRKY75, which is also affected by MdCML15 and MdJAZs in apples [ 46 ]. In wheat, there are 113 putative VQs in total; however, little is known about how these VQs impact leaf senescence [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway

Meng,

Lu,

Xia

et al. 2023

IJMS

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Leaf senescence is an important factor affecting the functional transition from nutrient assimilation to nutrient remobilization in crops. The senescence of wheat leaves is of great significance for its yield and quality. In the leaf senescence process, transcriptional regulation is a committed step in integrating various senescence-related signals. Although the plant-specific transcriptional regulation factor valine-glutamine (VQ) gene family is known to participate in different physiological processes, its role in leaf senescence is poorly understood. We isolated TaVQ25-A and studied its function in leaf senescence regulation. TaVQ25-A was mainly expressed in the roots and leaves of wheat. The TaVQ25-A-GFP fusion protein was localized in the nuclei and cytoplasm of wheat protoplasts. A delayed senescence phenotype was observed after dark and abscisic acid (ABA) treatment in TaVQ25-A-silenced wheat plants. Conversely, overexpression of TaVQ25-A accelerated leaf senescence and led to hypersensitivity in ABA-induced leaf senescence in Arabidopsis. A WRKY type transcription factor, TaWRKY133, which is tightly related to the ABA pathway and affects the expression of some ABA-related genes, was found to interact with TaVQ25-A both in vitro and in vivo. Results of this study indicate that TaVQ25-A is a positive regulator of ABA-related leaf senescence and can be used as a candidate gene for wheat molecular breeding.

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Section: Discussionmentioning

confidence: 99%

Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway

Meng,

Lu,

Xia

et al. 2023

IJMS

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“…The degree of coloration during fruit ripening is one of the important factors determining the marketability of fruit [6]. In general, red apples are favored more by consumers, who are largely reluctant to buy green apples.…”

Section: Introductionmentioning

confidence: 99%

MdSGR2 Negatively Regulates Chlorophyll Degradation in Apple

Xue,

Yao,

Kang

et al. 2024

Horticulturae

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The color of an apple is an important index of its appearance quality, which affects the fruit’s marketability and value. The green variety “Granny Smith” remains green when ripe, and the pigment in the peel is mainly chlorophyll. The chlorophyll in the peel of the yellow variety “Golden Delicious” degrades as the fruit ripens. To explore the different mechanisms of chlorophyll degradation in the “Golden Delicious” and “Granny Smith” varieties, transcriptomic analysis was performed on the mature peels of both cultivars. High-quality sequences totaling 48,206,940 and 48,796,646 raw bases, as well as 9632 differentially expressed genes (DEGs), were identified. A total of 1206 DEGs were classified in 118 KEGG metabolic pathways. Additionally, 3258 transcription factors from 428 gene families were predicted, and DEGs associated with chlorophyll degradation, including MdSGR2, MdNYC1 and MdHCAR, were identified. Subsequently, the MdSGR2 gene was isolated from the mature peel of “Granny Smith” apples. Subsequent subcellular localization analysis confirmed that MdSGR2 specifically targets chloroplasts. The transient overexpression of MdSGR2 was found to negatively regulate chlorophyll degradation, leading to the inhibition or deceleration of chlorophyll breakdown, thereby maintaining green coloration in the leaves and peel. Conversely, silencing MdSGR2 positively regulated chlorophyll degradation, resulting in chlorosis in the leaves and peel. These findings offer valuable insights into the regulatory mechanism governing apple fruit coloration and lays a solid foundation for further investigation in this field.

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“…SlVQ7 interacted with SlWRKY37 to mediate JA‐ and dark‐induced leaf senescence in tomato (Wang et al, 2022). The MdVQ10‐MdWRKY75 module promoted wound‐triggered leaf senescence by cooperating with a calmodulin‐like protein MdCML15 and the JA pathway in apple (Zhang et al, 2023). To date, although clues on how VQs participate in the regulation of leaf senescence are extremely limited, these studies prompted us to investigate the senescence‐associated roles of VQs.…”

Section: Introductionmentioning

confidence: 99%

AtVQ25 promotes salicylic acid‐related leaf senescence by fine‐tuning the self‐repression of AtWRKY53

Tan,

Zhao,

Gao

et al. 2024

JIPB

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Most mechanistic details of chronologically ordered regulation of leaf senescence are unknown. Regulatory networks centered on AtWRKY53 are crucial for orchestrating and integrating various senescence‐related signals. Notably, AtWRKY53 binds to its own promoter and represses transcription of AtWRKY53, but the biological significance and mechanism underlying this self‐repression remain unclear. In this study, we identified the VQ motif‐containing protein AtVQ25 as a cooperator of AtWRKY53. The expression level of AtVQ25 peaked at mature stage and was specifically repressed after the onset of leaf senescence. AtVQ25‐overexpressing plants and atvq25 mutants displayed precocious and delayed leaf senescence, respectively. Importantly, we identified AtWRKY53 as an interacting partner of AtVQ25. We determined that interaction between AtVQ25 and AtWRKY53 prevented AtWRKY53 from binding to W‐box elements on the AtWRKY53 promoter and thus counteracted the self‐repression of AtWRKY53. In addition, our RNA‐sequencing data revealed that the AtVQ25‐AtWRKY53 module is related to the salicylic acid (SA) pathway. Precocious leaf senescence and SA‐induced leaf senescence in AtVQ25‐overexpressing lines were inhibited by an SA pathway mutant, atsid2, and NahG transgenic plants; AtVQ25‐overexpressing/atwrky53 plants were also insensitive to SA‐induced leaf senescence. Collectively, we demonstrated that AtVQ25 directly attenuates the self‐repression of AtWRKY53 during the onset of leaf senescence, which is substantially helpful for understanding the timing of leaf senescence onset modulated by AtWRKY53.

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MdVQ10 promotes wound‐triggered leaf senescence in association with MdWRKY75 and undergoes antagonistic modulation of MdCML15 and MdJAZs in apple

Cited by 15 publications

References 76 publications

Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway

Wheat VQ Motif-Containing Protein VQ25-A Facilitates Leaf Senescence via the Abscisic Acid Pathway

MdSGR2 Negatively Regulates Chlorophyll Degradation in Apple

AtVQ25 promotes salicylic acid‐related leaf senescence by fine‐tuning the self‐repression of AtWRKY53

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