MAP4 regulates Tctex‐1 and promotes the migration of epidermal cells in hypoxia

Abstract

After acute wound formation, the oxygen supply is reduced, which results in the formation of an acute hypoxic microenvironment; whether this hypoxic microenvironment enhances epidermal cell migration and the underlying regulatory molecular mechanism of this effect are unclear. In this study, HaCaT cells were maintained under hypoxic (1% oxygen) or normoxic conditions. Methods including immunofluorescence staining, wound scratch assays, transwell assays, Western blotting and high- and low-expression lentiviral vector transfection were utilized to observe the changes in cell migration, microtubule dynamics and the expression levels of microtubule-associated protein (MAP) 4 and the light chain protein DYNLT1 (Tctex-1). The possible mechanisms were studied and discussed. The results showed that epidermal cell migration was enhanced during early hypoxia. Further experiments revealed that MAP4 regulates microtubule dynamics and promotes epidermal cell migration through Tctex-1. MAP4 and Tctex-1 play important roles in regulating the migration of epidermal cells under hypoxia. This evidence will provide a basis for further revealing the cellular and molecular mechanisms of local wound hypoxia and for promoting wound healing.

Identification of DYNLT1 associated with proliferation, relapse, and metastasis in breast cancer

“…DYNLT1 is responsible for the intracellular retrograde motility of vesicles and organelles along microtubules, binding to transport cargo, and is involved in apical cargo transport. It is reported that DYNLT1 plays an important role in many biological functions and diseases, such as Huntington's disease ( 8 ), fertilizing potential of human spermatozoa ( 9 ), migration of epidermal cells in hypoxia ( 10 ), autophagy lysosomal degradation ( 11 ), and several types of cancer. DYNLT1 has been reported to promote glioblastoma progression and is associated with tumor-node-metastasis (TNM) grade ( 12 ).…”

Research on the pathological mechanism of rectal adenocarcinoma based on DNA methylation

“…[ 23 ] Chen et al found that MAP4 affected the migration of epidermal cells by regulating the expression of Tctex-1. [ 24 ] In the occurrence and development of tumors, Xia et al found knockdown MAP4 could effectively inhibit the migration and invasion of lung adenocarcinoma cells, and the expression of MAP4 could be used as an independent indicator of prognostic survival of lung adenocarcinoma. [ 25 ] MAP4 is hypermethylated in prostate cancer.…”

MAP4 acts as an oncogene and prognostic marker and affects radioresistance by mediating epithelial–mesenchymal transition in lung adenocarcinoma

“…Lung cancer is one of the most common cancers and accounts for the highest number of cancer-related deaths worldwide (Césaire et al 2022 ). Lung adenocarcinoma, the most common subtype of lung cancer, is highly aggressive and generally has a poor prognosis (Chen et al 2018 ), with a low 5-year survival rate (Chen et al 2014 ; Cucchiarelli et al 2008 ). Radiation therapy is essential for patients with thoracic malignancies (Bacco et al 2011 ).…”