<scp>M</scp>ycobacterium wolinskyi Infection Confirmed by <i>rpo<scp>B</scp></i> Gene Sequencing

Jeong, Ji Hun; Seo, Yiel-Hea; Kim, Kyung Hee; Ahn, Jeong Yeal; Park, Pil-Hwan; Park, Young-Kil

doi:10.1002/jcla.21526

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…Contrary to several reported cases of SSTI caused by other NTM species [ 6 - 10 ], previously reported cases of M. wonlinskyi -related SSTI mostly described incidences following surgical procedures including hip prosthesis, peritoneal dialysis, transplantation, and heart surgery and posttraumatic events [ 3 , 11 - 13 ]. Infections have also been observed in immunocompromised patients [ 14 , 15 ] and in some cases of tissue necrosis involving subjacent bone [ 1 ]. However, M. wolinskyi infection complicating facial cosmetic procedure has not hitherto been reported, although there are increasing reports of SSTI caused by other RGM species involving cosmetic therapy such as M. fortuitum, M. abscessus, M. jacuzzi and M .…”

Section: Discussionmentioning

confidence: 99%

“…Culture and laboratory identification of NTM can be a time consuming process because the process relies on growth rate, pigmentation, and several biochemical tests [ 1 ]. Molecular analysis using 16S rRNA and rpoB gene sequencing and TB/NTM PCR screening has made the identification process more rapid and accurate [ 4 , 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among more than 140 species of nontuberculous mycobacteria (NTM), the rapidly growing mycobacteria (RGM), which are widely distributed in soil and water, is a clinically significant pathogen that causes various human diseases [ 1 ]. RGM, a type IV mycobacteria under the Runyon classification system, includes the Mycobacterium fortuitum group , Mycobacterium perginum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium immunogenum, and Mycobacterium smegmatis group [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Facial skin and soft tissue infection caused by Mycobacterium wolinskyi associated with cosmetic procedures

et al. 2013

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BackgroundMycobacteirum wolinskyi is a member of the Mycobacterium smegmatis group, which is less frequently found in clinical settings than other nontuberculous mycobacterium (NTM) species. However, its clinical significance has recently increased in opportunistic infections. This case is the first report of facial skin and soft tissue infection by M. wolinskyi complicating cosmetic procedures.Case presentationA 56-year-old Asian female patient with a history of receiving multiple facial cosmetic procedures over the preceding 2 years was admitted to our institution with swelling, local pain, and erythema on the right cheek. Mycobacteirum fortuitum complex isolated from a pus culture was identified as M. wolinskyi by rpoB sequencing. Metallic foreign bodies and abscess were detected by radiologic imaging. The pus was incised and drained. Treatment comprised clarithromycin (500 mg every 12 h), amikacin (200 mg every 8 h), and ciprofloxacin (400 mg every 6 h).ConclusionWe report the first case of facial skin and soft tissue infection with M. wolinskyi after multiple cosmetic procedures of filler injection and laser lipolysis. Increased occurrence of NTM infection in nosocomial settings suggests the importance of appropriate treatment including culturing and rpoB gene sequencing when patients who have undergone cosmetic procedures display symptoms and signs of soft tissue infection indicative of NTM infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Facial skin and soft tissue infection caused by Mycobacterium wolinskyi associated with cosmetic procedures

et al. 2013

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“…Mycobacterium wolinskyi is an uncommon, rapid growing NTM previously belonging to the Mycobacterium smegmatis group ( 2 ). Although fewer than 30 clinical cases have been described worldwide, M. wolinskyi is associated with bacteremia ( 3 , 4 ), peritonitis ( 5 ), infections associated with implants/prostheses ( 6 – 8 ), and postsurgical skin and soft tissue infections ( 9 – 11 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Mycobacterium wolinskyi , a Rapid-Growing Species of Nontuberculous Mycobacteria

et al. 2016

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“…Similarly, Mycobacterium goodie could not be differentiated from Mycobacterium wolinskyi due to identity between their 342bp rpoB fragment sequences. However, a different region in the complete rpoB gene sequence, or use of the hsp65 fragment, does allow the differentiation of M. goodie from M. wolinskyi [1,[44][45][46].…”

Section: Generation and Labeling Of Rpob Fragment Restriction Sites Fmentioning

confidence: 99%

A New Paradigm for Genus Mycobacterium Population Structure

Freidlin¹

2013

J Bacteriol Parasitol

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The genus Mycobacterium contains the pathogenic species tuberculosis and leprae, and additionally at least 148 sometimes opportunistic pathogenic species that occupy environmental niches. The genus Mycobacterium is in the order Actinomycetes along with other acid-fast genera with mycolic acids in their cell walls, such as Corynebacterium, Nocardia, and Rhodococcus. The DNA-dependent RNA polymerase subunit b gene rpoB, is relatively conserved among these bacteria. Sequence polymorphism in a 342bp fragment of rpoB is sufficient to differentiate among most species of Mycobacterium. However, construction of a robust and testable phylogeny or population structure based on this polymorphism, or polymorphism in other genomic regions, has remained elusive. This study presents a new paradigm for the resolution of genus Mycobacterium population structure. A minimum spanning tree (MST) was constructed on restriction enzyme site polymorphisms detected in silico for 5 enzymes, on the 342bp rpoB fragment obtained from GenBank data for 47 Mycobacterium species, and one species each from Corynebacterium, Nocardia and Rhodococcus. The MST was empirically divided into 3 regions. All the rapidly growing mycobacteria (RGM), with a halo of slowly growing mycobacteria (SGM), were found in MST-region 1. The correctness of the MST-regions model of genus Mycobacterium population structure was validated by statistically confirmed linkage disequilibrium of certain restriction site alleles. For certain alleles, SGM in MST-region 1 resembled the RGM of MST-region 1 more than the SGM of MST-region 3. The model provided a framework consistent with published genotypic and phenotypic observations, including expectations from comparative genomics and ribosomal RNA (rRNA) gene properties' distribution among species, and reported cladistic groupings of species. Corynebacterium diphtheriae, Nocardia nova and Rhodococcus equi belonged to MSTregions 2, 3, and 1 respectively. In conclusion, the MST-regions model of genus Mycobacterium population structure was robust, unambiguous, transparent for alleles, consistent with genotypes and phenotypes, and statistically testable.

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Mycobacterium wolinskyi Infection Confirmed by rpoB Gene Sequencing

Cited by 9 publications

References 12 publications

Facial skin and soft tissue infection caused by Mycobacterium wolinskyi associated with cosmetic procedures

Facial skin and soft tissue infection caused by Mycobacterium wolinskyi associated with cosmetic procedures

Draft Genome Sequence of Mycobacterium wolinskyi , a Rapid-Growing Species of Nontuberculous Mycobacteria

A New Paradigm for Genus Mycobacterium Population Structure

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