lncRNA RHPN1‐AS1 promotes the progression of endometrial cancer through the activation of ERK/MAPK pathway

Abstract

Aim This study aimed to investigate the function of long noncoding RNA RHPN1 antisense RNA 1 (lncRNA RHPN1‐AS1) in the progression of endometrial cancer (EC) and its underlying molecular mechanisms. Methods The RHPN1‐AS1 expression was measured by quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) in EC tissues and cells. The cell clones, proliferation, cell cycle, apoptosis, migration and invasion in Ishikawa and HEC‐1A cells were respectively measured by colony formation assay, cell counting kit‐8 assay (CCK‐8) assay, flow cytometry, wound healing assay and transwell assay. In addition, the protein expressions in Ishikawa and HEC‐1A cells were measured using western blot and Immunofluorescence assay. Results Our data showed the RHPN1‐AS1 expression was significantly upregulated in both EC tissues and cells. The expression of RHPN1‐AS1 was significantly correlated with FIGO stage, histological grade, and lymph node metastasis. Additionally, silencing RHPN1‐AS1 could inhibit proliferation, cell cycle progression, migration and invasion, and also promote apoptosis in Ishikawa and HEC‐1A cells. Moreover, silencing RHPN1‐AS1 could markedly elevate the expressions of caspase‐3 and Bax, but reduce the Bcl‐2 expression in Ishikawa and HEC‐1A cells. We also found that silencing RHPN1‐AS1 could significantly inhibit the phosphorylation of MEK and ERK in Ishikawa and HEC‐1A cells. After U0126 pretreatment, the inhibition effect of silencing RHPN1‐AS1 on the phosphorylation of MEK and ERK was strengthened. Conclusion Our study demonstrated that RHPN1‐AS1 could facilitate cell proliferation, migration and invasion, as well as inhibit apoptosis via activating ERK/MAPK pathway in EC.

Interference of long non‑coding RNA HAGLROS inhibits the proliferation and promotes the apoptosis of ovarian cancer cells by targeting miR‑26b‑5p

“…lncRNAs, which can serve as critical tumor promoters or suppressors, can affect the occurrence and progression of multiple types of diseases (23,24). HAGLROS is abnormally expressed in the serum of patients with pneumonia and it has been found to decrease apoptosis and autophagy in lipopolysaccharide-induced WI-38 cells (25).…”

S100A7 promotes endometrial carcinoma progression by activating the MAPK signaling pathway

“…In our study, we found that phosphorylation level of ERK, JNK and p38 was elevated by S100A7 overexpression and reduced by S100A7 silence. In EC, studies have demonstrated that the abnormally high expression of MAPK pathway-related proteins was clearly involved in the malignant progression and chemoresistance of EC [35,36]. Our finding suggested that S100A7 may activate ERK, JNK and p38 MAPK to accelerate EC progression.…”

Rhophilin rho GTPase binding protein 1-antisense RNA 1 (RHPN1-AS1) promotes ovarian carcinogenesis by sponging microRNA-485-5p and releasing DNA topoisomerase II alpha (TOP2A)

“…The lncRNA RHPN1-AS1 was previously reported to be upregulated in various cancers and is frequently associated with cancer pathophysiology [ 6 , 10 , 47 ]. In OC, robust lncRNA RHPN1-AS1 expression was detected in cancer tissues; it serves as an unfavorable prognosticator and promotes tumor cell proliferation and metastasis [ 14 ] Our study showed that RHPN1-AS1 expression was enhanced in OC tissues and cells.…”

“…Thus, targeting lncRNAs may interfere with cancer progression. Currently, RHPN1-AS1 is known to exhibit ceRNA activity against several antitumor miRNAs, such as miRNA-129, miR-625, miR-7-5p, and miR-485-5p, contributing to cancer tumorigenesis and progression [ 14 , 48–50 ]. Based on our bioinformatics prediction, we found that miR-485-5p is a novel target of RHPN1-AS.…”

“…Long non-coding RNAs (lncRNAs) > 200 nucleotides in length, play a crucial role in biological processes by regulating gene expression through sponge effects, even without protein-coding ability [ 5 ]. Multiple studies have reported that lncRNAs sponging microRNAs (miRNAs) regulate development, immune response, and tumorigenesis in various cancers [ 6–8 ]. Growing evidence has indicated that lncRNA RHPN1-AS1 plays important biological roles in different cancers, such as endometrial, colorectal, and gastric cancer [ 9–11 ].…”