Lnc‐RNA LINC01279 induces endometriosis via targeting of HOXA10

Abstract

Aim To explore the regulatory role and molecular mechanism of lncRNA‐LINC01279 in endometriosis (EMs). Methods Between September 2018 and July 2019, 20 EMs patients and 20 healthy subjects were recruited to detect the expression of lncRNA‐LINC01279 in EMs and in normal endometrium via qRT‐PCR. Autograft was used to establish EMs models on Spraque–Dawley (SD) rats, which was followed by taking volume measurements of EMs endometrium and observing pathological changes in the morphology of EMs via hematoxylin and eosin (H&E) staining. The qRT‐PCR technique was further carried out to determine mRNA expression of lncRNA‐LINC01279 and HOXA10 in the serum of EMs rats and LINC01279 shRNA‐transfected rats, while the protein expression of HOXA10 was determined using a Western blot. Results EMs patients presented with upregulation of lncRNA‐LINC01279 and downregulation of HOXA10 (p < 0.01 or 0.001). Online predictions further revealed that lncRNA‐LINC01279 regulated the expression of HOXA10 via miRNA‐135b. In EMs models, it was observed that there were a significantly enlarged endometrium and poor pathological morphology, significant upregulation of lncRNA‐LINC01279, and downregulation of miR‐135b and HOXA10 in serum (p < 0.05, 0.01 or 0.001). In the lncRNA‐LINC01279 shRNA group, EMs rats, following treatment, had a sharp decrease in the volume of EMs endometrium, and an improvement in pathological morphology, while lncRNA‐LINC01279 was downregulated, with upregulation of miR‐135b and HOXA10 (p < 0.05, 0.01 or 0.001). Conclusion LncRNA‐LINC01279, by the mechanism of targeting miR‐135b, has the potential to downregulate the expression of HOXA10, and therefore, can promote the development and progression of EMs.

Suppression of the long non-coding RNA LINC01279 triggers autophagy and apoptosis in lung cancer by regulating FAK and SIN3A

“…We found that either high or low expression of LINC01279 is correlated with a reduced OS rate in patients with LUAD. There is also evidence that upregulation of LINC01279 may be related to tumor invasion in gastric cancer [ 10 ]. Thus, it may represent a potential therapeutic target to prevent tumor growth.…”

“…Subsequent works suggested that its function could be associated with the pathogenesis of endometriosis [ 8 , 9 ]. Interestingly, more recent studies indicated a correlation between LINC01279 expression and progression of gastric cancer [ 10 ], suggesting a possible function of this IncRNA in regulating tumor growth. However, whether it plays a role in other cancers is unknown.…”

LncRNA HAGLR silencing inhibits IL-1β-induced chondrocytes inflammatory injury via miR-130a-3p/JAK1 axis

“…Non coding RNAs have been reported to play key roles in musculoskeletal conditions [6][7][8]. LncRNAs are a noncoding RNA with a length of more than 200 nucleotides [9]. The abnormal expression or function of lncRNA is closely related to the occurrence of human diseases, including breast cancer [10], liver diseases [11] and many other major diseases.…”

A critical appraisal of the circulating levels of differentially expressed microRNA in endometriosis

“…Many biomarkers have been proposed in the literature; however, none have been found, either alone or combined in a panel of markers, to yield sensitivity and specificity equivalent to laparoscopy [ 13–15 ]. In recent years, exosomes and their cargo [ 16 ], tissue concentrations of circular RNA [ 17 ], and long noncoding RNA [ 18 ] as well as circulating concentrations of microRNAs (miRNA) [ 19–21 ] have been documented in women with endometriosis compared with control populations and thus are thought to have potential value as candidate diagnostic markers of endometriosis [ 22 , 23 ]. Of these biomarkers, miRNA have received the greatest attention in the literature.…”