<scp>LINC</scp>00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21

Li, Gang; Wang, Changming; Wang, Yongbing; Xu, Bin; Zhang, Wenzhong

doi:10.1111/jcmm.13830

Cited by 32 publications

(27 citation statements)

References 27 publications

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“…lncRNA 00312 (Gene ID: 29931) attracted our attention based on its highly conserved characteristics and biological function. Previous studies have confirmed that lncRNA 00312 was significantly downregulated in both colorectal and lung cancers [20,21]. Overexpression of lncRNA 00312 can significantly inhibit the invasion and proliferation of lung cancer and colorectal cancer cells.…”

Section: Introductionmentioning

confidence: 77%

“…Numerous studies have confirmed that lncRNAs play an important role in many biological processes, such as development, cell growth, and tumorigenesis [4,25]. lncRNA 00312 is one of them; former studies have proved that it had the potential to be the biomarker and new drug for liver cancer [16], lung cancer [20], and colorectal cancer [21].…”

Section: Discussionmentioning

confidence: 99%

“…miR-197-3p is the target for lncRNA 00312 inhibiting the invasive and migrating ability of the bladder cancer cells [28] and thyroid cancer cells [29]. miR-21 is the target for lncRNA 00312 inhibiting proliferation and metastasis of colorectal cancer cells [21]. We would study the relationship between lncRNA 00312 and other miRNAs later.…”

Section: Discussionmentioning

confidence: 99%

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lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Zeng

Yaodong

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Previous studies have demonstrated that lncRNAs play functional roles in regulating cancer cell proliferation, invasion, and apoptosis. Recent studies confirmed that lncRNA 00312 has important biological functions in lung and colorectal cancer. However, the role of lncRNA 00312 in renal cell carcinoma (RCC) remains unclear. Our aim was to explore the function of lncRNA 00312 in RCC and its potential molecular mechanism. Methods. RCC cell lines A498 and ACHN were used as in vitro models in this study. RT-PCR was performed to determine lncRNA 00312, miR-34a-5p, and ASS1 mRNA expression. Proliferation and invasion were examined by CCK-8 and Transwell assay to confirm the function role of lncRNA 00312. Western blot analysis was used to examine the expression of apoptotic proteins Bax and Bcl-2. Results. lncRNA was significantly downregulated in RCC cells such as A498 and ACHN; the expression of lncRNA 00312 in RCC tissues was significantly lower than that in adjacent normal tissues. Patients with low expression of lncRNA 00312 have worse prognosis regarding pathological grade, tumor size, and TNM stage. Overexpression of lncRNA 00312 suppressed A498 and ACHN cell proliferation and invasion, while promoting apoptosis. Our study found that miR-34a-5p had the potential binding site with lncRNA 00312 and revealed the role of miR-34a-5p in RCC. Furthermore, we confirmed that lncRNA 00312 played its role with the participation of ASS1 and miR-34a-5p. Conclusion. lncRNA 00312 can inhibit RCC proliferation and invasion and promote apoptosis in vitro by suppressing miR-34a-5p and overexpressing ASS1. Our study demonstrated that the lncRNA 00312/miR-34a-5p/ASS1 axis may play a functional role in the progression of RCC; lncRNA 00312 abundance is a prognostic factor candidate for RCC survival, which provides new insights for RCC clinical treatment.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

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lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Zeng

Yaodong

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…LINC00312 also has been shown to diminish the aggressiveness of thyroid cancer through suppression of the PI3K/Akt pathway [15]. In addition, LINC00312 was found to inhibit colorectal cancer cells by binding to miR-21, therefore, increasing PTEN [16]. Another study showed that LINC00312 could downregulate cyclin B1 and induce G2-M cell cycle arrest in hepatocellular carcinoma cells, which downregulated cell proliferation and tumor progression in vivo [17].…”

Section: Discussionmentioning

confidence: 99%

LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis

Chen

Fang

et al. 2020

IJMS

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Oral submucous fibrosis (OSF) has been recognized as a precancerous disorder in the oral cavity. Great effort has been made to inhibit the malignant progression of OSF over the past decades, but the cure of this fibrosis disease has not been discovered. In the present study, we found that a long noncoding RNA, LINC00312, was upregulated in OSF tissues, and positively associated with several fibrosis factors, such as α-SMA, type I collagen, and fibronectin. As such, we sought to investigate the role of LINC00312 in OSF progression and identify its interacting factor that mediated oral fibrogenesis. Our results showed that the inhibition of LINC00312 downregulated the myofibroblast activities, including collagen gel contractility, transwell migration, and wound healing, as well as the gene expression of myofibroblast markers. We verified that YBX1 was a downstream factor of LINC00312 and revealed that the downregulation of YBX1 repressed the gene expression of α-SMA and p-Smad2 along with the reduced myofibroblast phenotypes. Most importantly, we demonstrated that the LINC00312-induced myofibroblast activities were reverted by the knockdown of YBX1, suggesting that the LINC00312-mediated myofibroblast transdifferentiation was through YBX1. Collectively, our findings revealed that the LINC00312/ YBX1 axis may serve as a target for the development of therapies against OSF.

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“…Previous studies have indicated that lncRNAs are associated with CRC progression and prognosis. lncRNA BRAF non-coding RNA was demonstrated to promote CRC tumorigenesis and enhance adriamycin resistance, and LINC00312 was indicated to repress CRC cell proliferation and metastasis via miR-21 (18,19). However, the expression pattern and functional roles of a large number of lncRNAs remain undetermined.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of differentially expressed lncRNAs as diagnostic and prognostic markers for colorectal cancer

Zhang

Song

et al. 2019

Exp Ther Med

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Colorectal cancer (CRC) is the third most common type of cancer worldwide. Recent studies had revealed the important roles of long non-coding RNAs (lncRNAs) in a variety of human cancers, including CRC. However, the molecular mechanisms associated with CRC remain largely undetermined. In the current study, the GSE21510 dataset was analyzed to identify differentially expressed mRNAs and lncRNAs in CRC samples. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway bioinformatics analysis. Furthermore, protein-protein interaction networks were constructed to reveal interactions among differentially expressed proteins. Kaplan-Meier analysis was subsequently performed to determine the association between key lncRNA expression and the overall survival of patients with CRC. A total of 107 upregulated lncRNAs and 43 downregulated lncRNAs were identified in CRC. A lncRNA mediated co-expression network was also constructed in CRC. Bioinformatics analysis indicated that lncRNAs were associated with a series of biological processes, including 'xenobiotic glucuronidation', 'rRNA processing', 'sister chromatid cohesion', 'cell proliferation', 'mitotic nuclear division' and 'cell cycle regulation'. Furthermore, a higher expression of small nucleolar RNA host gene 17, tetratricopeptide repeat domain 2B-antisense RNA (AS) 1, erythrocyte membrane protein band 4.1 like 4A-AS2, deleted in lymphocytic leukemia 2, and a lower expression of muscle blind like splicing regulator 1-AS1 and LOC389332 were associated with shorter overall survival time in CRC samples. The present study provides useful information that can be used in the identification of novel biomarkers for CRC.

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LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21

Cited by 32 publications

References 27 publications

lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis

Comprehensive analysis of differentially expressed lncRNAs as diagnostic and prognostic markers for colorectal cancer

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