LC/MS analysis of mushrooms provided new insights into dietary management of diabetes mellitus in rats

Abstract

Mushrooms possess antihyperglycemic effect on diabetic individuals due to their nonfibrous and fibrous bioactive compounds. This study aimed to reveal the effect of different types of mushrooms on plasma glucose level and gut microbiota composition in diabetic individuals. The effects of five different mushroom species ( Ganoderma lucidum , GLM; Pleurotus ostreatus , POM; Pleurotus citrinopileatus , PCM; Lentinus edodes , LEM; or Hypsizigus marmoreus , HMM) on alloxan‐induced diabetic rats were investigated in this study. The results indicated that LEM and HMM treatments showed lower plasma glucose levels. For the microbiota composition, ACE , Chao1 , Shannon , and Simpson were significantly affected by PCM and LEM treatments ( p < .05), while ACE, Shannon, and Simpson indexes were affected by HMM treatment ( p < .01). Simpson index was affected in positive control (C+) and POM groups. All these four indices were lower in GLM treatment ( p < .05). Dietary supplementation of mushrooms reduced plasma glucose level directly through mushrooms' bioactive compounds (agmatine, sphingosine, pyridoxine, linolenic, and alanine) and indirectly through stachyose (oligosaccharide) and gut microbiota modulation. In conclusion, LEM and HMM can be used as food additives to improve plasma glucose level and gut microbiome composition in diabetic individuals.

Exploring the Effects of Different Drying Methods on Related Differential Metabolites of Pleurotus citrinopileatus Singer Based on Untargeted Metabolomics

“…Fungal metabolites have historically been categorized into primary, that is, metabolites essential for growth and reproduction, and secondary, which are considered to be rich sources of potential drug candidates and other useful substances [ 3 , 4 ]. According to the literature, the fruit body of PCS is rich in metabolites such as polysaccharides, phenols, flavonoids, terpenoids and alkaloids [ 5 , 6 ], with potential pharmacological activity against neoplastic [ 3 ], displays antioxidant activities [ 3 ], immune regulatory effects [ 7 ], and it has a key role in regulating blood sugar [ 8 ] and lowering blood pressure and cholesterol [ 9 ]. Meanwhile, the lectin extract from the PCS fruit body exhibits potent anti-inflammatory effects [ 10 ].…”

Ganoderma lucidum: Novel Insight into Hepatoprotective Potential with Mechanisms of Action

“…As a source of distinctive bioactive metabolites, which grants it a number of attributes, it has been demonstrated to be efficient in the treatment and management of a variety of diseases [4]. G. lucidum has demonstrated potent anticancer [5,6], anti-inflammatory [7], hepatoprotective [8], antidiabetic [9], cardioprotective [10], immunomodulatory [11], antioxidant [12], and antiaging [13] activities. Due to its distinct potential as a medicine, there is increased demand in the food sector for it to be used as a significant source for nutrient supplements [14].…”