<scp>LBD</scp>18 uses a dual mode of a positive feedback loop to regulate <scp>ARF</scp> expression and transcriptional activity in Arabidopsis

Pandey, Shashank K.; Lee, Han Woo; Kim, Min Jung; Cho, Chuloh; Oh, Eunkyoo; Kim, Jungmook

doi:10.1111/tpj.13945

Cited by 32 publications

(20 citation statements)

References 76 publications

(124 reference statements)

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“…It was shown that the auxin influx carriers AUXIN1 (AUX1) and LIKE-AUXIN3 (LAX3) are required for the auxin-induced expression of AtLBD16 and AtLBD18 to control LR development during various stages in Arabidopsis [98][99][100]. The ARF7/19-AtLBD16/18 transcriptional module was also identified as playing an important role in AR formation in Arabidopsis [101]. In Arabidopsis, initiation of LR formation starts from the founder cells' asymmetric division, and through subsequent cell proliferation and differentiation to form new primordia.…”

Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

“…LR initiation requires the sequential induction of transcription factor gene AtLBD16 and its target PUCHI [102]. AtLBD18 and ARFs form a double positive feedback loop, as AtLBD18 can not only bind to the ARF19 promoter directly but also interacts with ARF7 and ARF19 [101]. These feedback loops may contribute to the continued LR growth in response to auxin in Arabidopsis [101].…”

Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

“…AtLBD18 and ARFs form a double positive feedback loop, as AtLBD18 can not only bind to the ARF19 promoter directly but also interacts with ARF7 and ARF19 [101]. These feedback loops may contribute to the continued LR growth in response to auxin in Arabidopsis [101]. The coiled-coil motifs in AtLBD16 and AtLBD18 transcription factors determine their DNA-binding properties, including DNA-binding diversity, specificity and affinity, which functions in the transcriptional regulation of different cellular processes and biological pathways in Arabidopsis [103].…”

Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

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Phylogeny and Functions of LOB Domain Proteins in Plants

Zhang

et al. 2020

IJMS

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Lateral organ boundaries (LOB) domain (LBD) genes, a gene family encoding plant-specific transcription factors, play important roles in plant growth and development. At present, though there have been a number of genome-wide analyses on LBD gene families and functional studies on individual LBD proteins, the diverse functions of LBD family members still confuse researchers and an effective strategy is required to summarize their functional diversity. To further integrate and improve our understanding of the phylogenetic classification, functional characteristics and regulatory mechanisms of LBD proteins, we review and discuss the functional characteristics of LBD proteins according to their classifications under a phylogenetic framework. It is proved that this strategy is effective in the anatomy of diverse functions of LBD family members. Additionally, by phylogenetic analysis, one monocot-specific and one eudicot-specific subclade of LBD proteins were found and their biological significance in monocot and eudicot development were also discussed separately. The review will help us better understand the functional diversity of LBD proteins and facilitate further studies on this plant-specific transcription factor family.

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Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

Section: Functions Of Lbd Proteins In Class Ib Cladementioning

confidence: 99%

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Phylogeny and Functions of LOB Domain Proteins in Plants

Zhang

et al. 2020

IJMS

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“…This construct was subcloned into the destination vector pGWB516 (Nakagawa, Shimane University, Japan) by an LR recombination reaction, and was then transformed into the lbd16 mutant by Agrobacterium-mediated transformation, generating Pro LBD16 :LBD16:4xMyc / lbd16 Arabidopsis. Pro LBD18 :LBD18:3xHA / lbd18 Arabidopsis generated from a previous study was used [60]. Pro 35S :LBD16:GR / arf7 arf19 and Pro 35S :LBD18:GR / arf7 arf19 transgenic mutants were generated by crossing arf7–1 arf19–1 (female) with Pro 35S :LBD16:GR (male) or Pro 35S :LBD18:GR (male) [37].…”

Section: Methodsmentioning

confidence: 99%

LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis

et al. 2019

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BackgroundAdventitious root (AR) formation is a complex genetic trait, which is controlled by various endogenous and environmental cues. Auxin is known to play a central role in AR formation; however, the mechanisms underlying this role are not well understood.ResultsIn this study, we showed that a previously identified auxin signaling module, AUXIN RESPONSE FACTOR(ARF)7/ARF19-LATERAL ORGAN BOUNDARIES DOMAIN(LBD)16/LBD18 via AUXIN1(AUX1)/LIKE-AUXIN3 (LAX3) auxin influx carriers, which plays important roles in lateral root formation, is involved in AR formation in Arabidopsis. In aux1, lax3, arf7, arf19, lbd16 and lbd18 single mutants, we observed reduced numbers of ARs than in the wild type. Double and triple mutants exhibited an additional decrease in AR numbers compared with the corresponding single or double mutants, respectively, and the aux1 lax3 lbd16 lbd18 quadruple mutant was devoid of ARs. Expression of LBD16 or LBD18 under their own promoters in lbd16 or lbd18 mutants rescued the reduced number of ARs to wild-type levels. LBD16 or LBD18 fused to a dominant SRDX repressor suppressed promoter activity of the cell cycle gene, Cyclin-Dependent Kinase(CDK)A1;1, to some extent. Expression of LBD16 or LBD18 was significantly reduced in arf7 and arf19 mutants during AR formation in a light-dependent manner, but not in arf6 and arf8. GUS expression analysis of promoter-GUS reporter transgenic lines revealed overlapping expression patterns for LBD16, LBD18, ARF7, ARF19 and LAX3 in AR primordia.ConclusionThese results suggest that the ARF7/ARF19-LBD16/LBD18 transcriptional module via the AUX1/LAX3 auxin influx carriers plays an important role in AR formation in Arabidopsis.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1659-4) contains supplementary material, which is available to authorized users.

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“…ARFs have important functions in lateral root growth 34,35 , embryogenesis 36,37 , leaf expansion 38,39 and fruit development 40,41 , and they have also been studied in response to different auxin concentrations 42 . In an Arabidopsis transcriptomic study, Paponov et al 42 found that in response to different concentrations of auxins, from very low (0.1 μM) to very high (10 μM), ARFs 4, 16 and 19 were up-regulated in response to auxin.…”

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confidence: 99%

Auxin perception in Agave is dependent on the species’ Auxin Response Factors

Cancino-García

Ramírez-Prado

De‐la‐Peña

2020

Sci Rep

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Auxins are one of the most important and studied phytohormones in nature. Auxin signaling and perception take place in the cytosol, where the auxin is sensed. then, in the nucleus, the auxin response factors (ARf) promote the expression of early-response genes. it is well known that not all plants respond to the same amount and type of auxins and that the response can be very different even among plants of the same species, as we present here. Here we investigate the behavior of ARf in response to various auxins in Agave angustifolia Haw., A. fourcroydes Lem. and A. tequilana Weber var. Azul. By screening the available database of A. tequilana genes, we have identified 32 ARF genes with high sequence identity in the conserved domains, grouped into three main clades. A phylogenetic tree was inferred from alignments of the 32 Agave ARF protein sequences and the evolutionary relationship with other species was analyzed. AteqARF 4, 15, 21, and 29 were selected as a representative diverse sample coming from each of the different subclades that comprise the two main clades of the inferred phylogenetic reconstruction. these ARFs showed differential species-specific expression patterns in the presence of indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Interestingly, A. angustifolia showed different phenotypes in the presence and absence of auxins. In the absence of auxin, A. angustifolia produces roots, while shoots are developed in the presence of iAA. However, in the presence of 2,4-D, the plant meristem converts into callus. According to our results, it is likely that AteqARF15 participates in this outcome.

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LBD18 uses a dual mode of a positive feedback loop to regulate ARF expression and transcriptional activity in Arabidopsis

Cited by 32 publications

References 76 publications

Phylogeny and Functions of LOB Domain Proteins in Plants

Phylogeny and Functions of LOB Domain Proteins in Plants

LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis

Auxin perception in Agave is dependent on the species’ Auxin Response Factors

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