<scp>l</scp>‐Theanine inhibits proinflammatory PKC/ERK/ICAM‐1/IL‐33 signaling, apoptosis, and autophagy formation in substance P‐induced hyperactive bladder in rats

Tsai, Wen Hsin; Wu, Chung Hsin; Yu, Hong‐Jeng; Chien, Chiang Ting

doi:10.1002/nau.22965

Cited by 26 publications

(20 citation statements)

References 39 publications

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“…L-theanine, namely N-ethyl-γ-glutamine, is a unique non-protein amino acid in tea [ 8 ] that can be degraded by glutaminase to glutamate and ethylamine in vivo [ 9 ]. It has many physiological functions, such as anti-oxidative stress [ 10 ], regulating immune response [ 11 ], and relaxing neural tension [ 12 ]. L-theanine alone or in combination with L-cystine administration can restore reduced glutathione (GSH) synthesis in mammals [ 13 ], further enhancing the antioxidant capacity [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

Yan

Tang

et al. 2018

Med Sci Monit

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BackgroundL-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro.Material/MethodsThe certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 μM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed. Quantitative evaluation of apoptosis was performed by flow cytometric analysis. Nuclear morphology of the cells was monitored by 4′,6-diamidino-2-phenylindole staining. Expression of Caspase-3, poly ADP-ribose polymerase (PARP), c-Jun N-terminal kinase (JNK), and mitogen-activated protein kinase p38 was assayed by Western blot.ResultsCompared to the H2O2 treatment, all doses of L-theanine treatments increased the cell viability, glutathione level, and the activities of glutathione peroxidase and superoxide dismutase (P<0.001). The contents of reactive oxygen species, nitric oxide, and oxidized glutathione were decreased by L-theanine treatments (P<0.001). Meanwhile, L-theanine treatments decreased the apoptosis ratio of H2O2-induced H9C2 cells (P<0.001). Pro-Caspase-3 expression was upregulated and cleavaged-PARP expression was inhibited by L-theanine (P<0.001). However, the phosphorylation of JNK and p38 was not affected by L-theanine treatments (P>0.05).ConclusionsThese data indicate that L-theanine pretreatment prevents H2O2-induced apoptosis in H9C2 cells, probably via antioxidant capacity improvement. Therefore, it might be a promising potential drug candidate for prophylaxis of ischemia/reperfusion-induced heart diseases.

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Section: Introductionmentioning

confidence: 99%

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

Yan

Tang

et al. 2018

Med Sci Monit

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“…IL-33 as an alarmin was widely discussed about its functions in several different types of autoimmune diseases [ 19 , 20 ]. Some researches show that IL-33 was a proinflammatory factor [ 16 , 21 ], while others prove that it provokes a remedy mechanism [ 22 – 24 ]. In our study, we first explored how rIL-33 affects immune cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1^-/- Mouse through Promoting M2 Macrophage Polarization

You

Zhang

Wang

et al. 2020

Journal of Immunology Research

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This study was to identify functions of ILC2, a newly found innate lymphoid cell which mainly locates in mucosa organs like lungs and intestines, in IBD. We injected rIL-33 protein to C57/BL6 mouse to explore how IL-33 induces ILC2 proliferation. The results showed that ILC2 reached a proliferation peak at day 5 and expressed multiple surface markers like CD127, C-kit, CD69, CD44, ST2, CD27, DR3, MHCII, and CD90.2. ILC2 also expressed high quantity of IL-13 and IL-5 and few IL-17A which indicates a potentially immunological function in IBD development. Afterwards, we transferred sort purified ILC2 to Rag1-/- mouse given DSS to induce acute colitis in order to explore the innate function of ILC2. Data showed that ILC2 alleviates DSS-induced acute innate colitis by repairing epithelial barrier and restore body weight. Furthermore, we found that ILC2 can cause macrophages polarizing to M2 macrophages in the gut. Therefore, we concluded that ILC2 played a therapeutic role in mouse acute colitis.

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“…Furthermore, higher levels of oxidative stress and apoptosis were observed in the pelvic ischemia group, which showed detrusor hyperactivity [29]. Tsai et al [30] also showed increased levels of oxidative stress, proinflammatory PKC/ERK/NF-κB/ICAM-1/IL-33 signaling, and apoptosis in OAB rats induced by substance P.…”

Section: Injmentioning

confidence: 92%

The Role of Apoptosis in Detrusor Contractility

Kim

2020

Int Neurourol J

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Apoptosis has been found in bladder affected by various types of voiding dysfunction. In animal studies, higher levels of apoptosis were observed in conditions of both detrusor overactivity and underactivity than in normal bladders. However, it has been difficult to establish the exact underlying mechanism of apoptosis in these conditions and to find new therapeutic targets because the causes of voiding dysfunction are diverse and the coexistence of various types of voiding problems is common. Furthermore, the lack of studies of the human detrusor contributes to our incomplete understanding of these issues. Therefore, this revies discuss the role of apoptosis in detrusor contractility based on previous studies.

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l‐Theanine inhibits proinflammatory PKC/ERK/ICAM‐1/IL‐33 signaling, apoptosis, and autophagy formation in substance P‐induced hyperactive bladder in rats

Cited by 26 publications

References 39 publications

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1^-/- Mouse through Promoting M2 Macrophage Polarization

The Role of Apoptosis in Detrusor Contractility

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l‐Theanine inhibits proinflammatory PKC/ERK/ICAM‐1/IL‐33 signaling, apoptosis, and autophagy formation in substance P‐induced hyperactive bladder in rats

Cited by 26 publications

References 39 publications

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

L-Theanine Protects H9C2 Cells from Hydrogen Peroxide-Induced Apoptosis by Enhancing Antioxidant Capability

ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1-/- Mouse through Promoting M2 Macrophage Polarization

The Role of Apoptosis in Detrusor Contractility

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ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1^-/- Mouse through Promoting M2 Macrophage Polarization